Analysis of nucleosome assembly and histone exchange using antibodies specific for acetylated H4

Using antibodies that specifically recognize the acetylated forms of histone H4, we show that it is possible to immunoprecipitate newly assembled (acetylated) nucleosomes. Newly replicated HeLa cell chromatin was labeled for 5-30 min with [3H]thymidine in the presence of sodium butyrate (thus inhibi...

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Veröffentlicht in:	Biochemistry (Easton) 1993-12, Vol.32 (49), p.13605-13614
Hauptverfasser:	Perry, Carolyn A, Dadd, Christopher A, Allis, C. David, Annunziato, Anthony T
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylation Binding, Competitive Biological and medical sciences Butyrates - pharmacology Butyric Acid Chromatin - drug effects Chromatin - metabolism Chromatin. Chromosome DNA - biosynthesis DNA Replication Ethidium Fundamental and applied biological sciences. Psychology HeLa Cells Histones - immunology Histones - metabolism Humans Immunosorbent Techniques Molecular and cellular biology Molecular genetics Nucleosomes - metabolism Staining and Labeling
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