Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA

Biolistic (biological ballistic) and protoplast-mediated procedures were compared as methods for transforming strains of Gliocladium virens and Trichoderma harzianum. For biolistic transformation, conidia were bombarded using a helium-driven biolistic device to accelerate M5 tungsten particles coate...

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Veröffentlicht in:	Current genetics 1993-10, Vol.24 (4), p.349-356
Hauptverfasser:	LORITO, M, HAYES, C. K, DI PIETRO, A, HARMAN, G. E
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Blotting, Southern Cell Nucleus - metabolism Classical genetics, quantitative genetics, hybrids DNA, Fungal Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Genome, Fungal Gliocladium virens Mitosporic Fungi - genetics Plasmids Protoplasts - metabolism Thallophyta, bryophyta Transformation, Genetic Trichoderma - genetics Trichoderma harzianum Vegetals
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