Regulation of Proliferation-Specific and Differentiation-Specific Genes during Senescence of Human Epidermal Keratinocyte and Mammary Epithelial Cells
The expression of proliferation-associated genes, cdc2 and E2F-1 and squamous-specific genes transglutaminase type I and cornifin were examined in senescing human epidermal keratinocytes. Cultured keratinocytes underwent 34 population doublings before senescing. Senescence in keratinocytes was chara...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1993-11, Vol.197 (1), p.46-54 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Saunders, N.A. Smith, R.J. Jetten, A.M. |
| description | The expression of proliferation-associated genes, cdc2 and E2F-1 and squamous-specific genes transglutaminase type I and cornifin were examined in senescing human epidermal keratinocytes. Cultured keratinocytes underwent 34 population doublings before senescing. Senescence in keratinocytes was characterised by reduced thymidine incorporation, a change in morphology and the inability of cells to undergo mitosis. This was accompanied by downregulation of cdc2 and E2F-1 mRNA′s. In addition, senescing keratinocytes started to express genes such as cornifin, that are specific for squamous differentiation. These changes were similar to those observed in keratinocytes induced to differentiate with phorbol ester or by confluence. E2F-1, cdc2 and cornifin were similarly altered in senescing human mammary epithelial cells. Our data suggest that events regulating senescence may also be linked to squamous differentiation. |
| doi_str_mv | 10.1006/bbrc.1993.2439 |
| format | Article |
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Cultured keratinocytes underwent 34 population doublings before senescing. Senescence in keratinocytes was characterised by reduced thymidine incorporation, a change in morphology and the inability of cells to undergo mitosis. This was accompanied by downregulation of cdc2 and E2F-1 mRNA′s. In addition, senescing keratinocytes started to express genes such as cornifin, that are specific for squamous differentiation. These changes were similar to those observed in keratinocytes induced to differentiate with phorbol ester or by confluence. E2F-1, cdc2 and cornifin were similarly altered in senescing human mammary epithelial cells. Our data suggest that events regulating senescence may also be linked to squamous differentiation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.2439</identifier><identifier>PMID: 7902715</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Ageing, cell death ; Base Sequence ; Biological and medical sciences ; Biomarkers ; Breast - cytology ; Breast - physiology ; CDC2 Protein Kinase - biosynthesis ; CDC2 Protein Kinase - genetics ; Cell Differentiation - physiology ; Cell Division - genetics ; Cell physiology ; Cells, Cultured ; Cellular Senescence - physiology ; Cornified Envelope Proline-Rich Proteins ; Down-Regulation ; Epithelium - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Keratinocytes - physiology ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; Molecular and cellular biology ; Molecular Sequence Data ; Transglutaminases - biosynthesis ; Transglutaminases - genetics</subject><ispartof>Biochemical and biophysical research communications, 1993-11, Vol.197 (1), p.46-54</ispartof><rights>1993 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-f222d7a8d0e04d0084ab4260e861c36b3b4c13760dc762f61e55465394c069ad3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1993.2439$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3829722$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7902715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saunders, N.A.</creatorcontrib><creatorcontrib>Smith, R.J.</creatorcontrib><creatorcontrib>Jetten, A.M.</creatorcontrib><title>Regulation of Proliferation-Specific and Differentiation-Specific Genes during Senescence of Human Epidermal Keratinocyte and Mammary Epithelial Cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The expression of proliferation-associated genes, cdc2 and E2F-1 and squamous-specific genes transglutaminase type I and cornifin were examined in senescing human epidermal keratinocytes. Cultured keratinocytes underwent 34 population doublings before senescing. Senescence in keratinocytes was characterised by reduced thymidine incorporation, a change in morphology and the inability of cells to undergo mitosis. This was accompanied by downregulation of cdc2 and E2F-1 mRNA′s. In addition, senescing keratinocytes started to express genes such as cornifin, that are specific for squamous differentiation. These changes were similar to those observed in keratinocytes induced to differentiate with phorbol ester or by confluence. E2F-1, cdc2 and cornifin were similarly altered in senescing human mammary epithelial cells. Our data suggest that events regulating senescence may also be linked to squamous differentiation.</description><subject>Ageing, cell death</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Breast - cytology</subject><subject>Breast - physiology</subject><subject>CDC2 Protein Kinase - biosynthesis</subject><subject>CDC2 Protein Kinase - genetics</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Division - genetics</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Cellular Senescence - physiology</subject><subject>Cornified Envelope Proline-Rich Proteins</subject><subject>Down-Regulation</subject><subject>Epithelium - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Keratinocytes - physiology</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Transglutaminases - biosynthesis</subject><subject>Transglutaminases - genetics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcGL1DAYxYMo67h69Sb0IN46fknbtDnKuO6KK4qr4C2kyZc1kqZj0gr7j_j3mswMexA8heT98ni8R8hzClsKwF-PY9RbKkSzZW0jHpANBQE1o9A-JBvIRM0E_f6YPEnpJwClLRdn5KwXwHrabcifL3i7erW4OVSzrT7H2TuL8fBQ3-xRO-t0pYKp3jqbBQyL-0e8xICpMmt04ba6KReNQWOxu1onFaqLvTMYJ-WrDwfnMOu7BQ-mH9U0qXhXkOUHepeZHXqfnpJHVvmEz07nOfn27uLr7qq-_nT5fvfmutYNH5baMsZMrwYDCK0BGFo1towDDpxmYmzGVtOm52B0z5nlFLuu5V0jWg1cKNOck1dH332cf62YFjm5HN97FXBek-w5Bcqgy-D2COo4pxTRyn10JbqkIMsQsgwhyxCyDJE_vDg5r-OE5h4_NZ_1lyddJa28jSpol-6xZmCiZyxjwxHD3MJvh1Em7Uq9xkXUizSz-1-Cv5Wwph4</recordid><startdate>19931130</startdate><enddate>19931130</enddate><creator>Saunders, N.A.</creator><creator>Smith, R.J.</creator><creator>Jetten, A.M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931130</creationdate><title>Regulation of Proliferation-Specific and Differentiation-Specific Genes during Senescence of Human Epidermal Keratinocyte and Mammary Epithelial Cells</title><author>Saunders, N.A. ; Smith, R.J. ; Jetten, A.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-f222d7a8d0e04d0084ab4260e861c36b3b4c13760dc762f61e55465394c069ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Ageing, cell death</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Breast - cytology</topic><topic>Breast - physiology</topic><topic>CDC2 Protein Kinase - biosynthesis</topic><topic>CDC2 Protein Kinase - genetics</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Division - genetics</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Cellular Senescence - physiology</topic><topic>Cornified Envelope Proline-Rich Proteins</topic><topic>Down-Regulation</topic><topic>Epithelium - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Keratinocytes - physiology</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Transglutaminases - biosynthesis</topic><topic>Transglutaminases - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saunders, N.A.</creatorcontrib><creatorcontrib>Smith, R.J.</creatorcontrib><creatorcontrib>Jetten, A.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saunders, N.A.</au><au>Smith, R.J.</au><au>Jetten, A.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of Proliferation-Specific and Differentiation-Specific Genes during Senescence of Human Epidermal Keratinocyte and Mammary Epithelial Cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-11-30</date><risdate>1993</risdate><volume>197</volume><issue>1</issue><spage>46</spage><epage>54</epage><pages>46-54</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The expression of proliferation-associated genes, cdc2 and E2F-1 and squamous-specific genes transglutaminase type I and cornifin were examined in senescing human epidermal keratinocytes. Cultured keratinocytes underwent 34 population doublings before senescing. Senescence in keratinocytes was characterised by reduced thymidine incorporation, a change in morphology and the inability of cells to undergo mitosis. This was accompanied by downregulation of cdc2 and E2F-1 mRNA′s. In addition, senescing keratinocytes started to express genes such as cornifin, that are specific for squamous differentiation. These changes were similar to those observed in keratinocytes induced to differentiate with phorbol ester or by confluence. E2F-1, cdc2 and cornifin were similarly altered in senescing human mammary epithelial cells. Our data suggest that events regulating senescence may also be linked to squamous differentiation.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>7902715</pmid><doi>10.1006/bbrc.1993.2439</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1993-11, Vol.197 (1), p.46-54 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Ageing, cell death Base Sequence Biological and medical sciences Biomarkers Breast - cytology Breast - physiology CDC2 Protein Kinase - biosynthesis CDC2 Protein Kinase - genetics Cell Differentiation - physiology Cell Division - genetics Cell physiology Cells, Cultured Cellular Senescence - physiology Cornified Envelope Proline-Rich Proteins Down-Regulation Epithelium - physiology Female Fundamental and applied biological sciences. Psychology Humans Keratinocytes - physiology Membrane Proteins - biosynthesis Membrane Proteins - genetics Molecular and cellular biology Molecular Sequence Data Transglutaminases - biosynthesis Transglutaminases - genetics |
| title | Regulation of Proliferation-Specific and Differentiation-Specific Genes during Senescence of Human Epidermal Keratinocyte and Mammary Epithelial Cells |
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