A Vital Stain for the Golgi Apparatus
The Golgi complex, a membranous organelle with important functions in membrane traffic and macromolecular synthesis, has been stained in living cells with a fluorescent sphingolipid. Cells were first incubated with liposomes containing N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl sphingosin...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1985-05, Vol.228 (4700), p.745-747 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Lipsky, Naomi Geller Pagano, Richard E. |
| description | The Golgi complex, a membranous organelle with important functions in membrane traffic and macromolecular synthesis, has been stained in living cells with a fluorescent sphingolipid. Cells were first incubated with liposomes containing N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl sphingosine (C$_{6}$-NBD-ceramide), or with a bovine serum albumin complex of the fluorescent lipid, and then examined by fluorescence microscopy. An intensely fluorescent perinuclear structure was identified as the Golgi apparatus by its colocalization with known Golgi markers in fixed cells. C$_{6}$-NBD-ceramide was used to observe the morphology of the Golgi apparatus in living cells in the presence or absence of monensin or Colcemid, and during mitosis. In all cases, C$_{6}$-NBD-ceramide revealed a Golgi apparatus in the living cell that was identical to that obtained with conventional procedures that require fixation. |
| doi_str_mv | 10.1126/science.2581316 |
| format | Article |
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Cells were first incubated with liposomes containing N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl sphingosine (C$_{6}$-NBD-ceramide), or with a bovine serum albumin complex of the fluorescent lipid, and then examined by fluorescence microscopy. An intensely fluorescent perinuclear structure was identified as the Golgi apparatus by its colocalization with known Golgi markers in fixed cells. C$_{6}$-NBD-ceramide was used to observe the morphology of the Golgi apparatus in living cells in the presence or absence of monensin or Colcemid, and during mitosis. In all cases, C$_{6}$-NBD-ceramide revealed a Golgi apparatus in the living cell that was identical to that obtained with conventional procedures that require fixation.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.2581316</identifier><identifier>PMID: 2581316</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: The American Association for the Advancement of Science</publisher><subject>3T3 cells ; 4-Chloro-7-nitrobenzofurazan - analogs & derivatives ; Animals ; Biological and medical sciences ; Cell lines ; Cell research ; Ceramides ; Cricetinae ; Cricetulus ; Cultured cells ; Cytological research ; Diverse techniques ; Fibroblasts ; Fibroblasts - ultrastructure ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Golgi apparatus ; Golgi Apparatus - drug effects ; Golgi Apparatus - ultrastructure ; Hepatocytes ; Humans ; Lipids ; Liposomes ; Microscopy, Fluorescence ; Mitosis ; Molecular and cellular biology ; Serum Albumin, Bovine ; Staining and Labeling - methods</subject><ispartof>Science (American Association for the Advancement of Science), 1985-05, Vol.228 (4700), p.745-747</ispartof><rights>Copyright 1985 The American Association for the Advancement of Science</rights><rights>1985 INIST-CNRS</rights><rights>COPYRIGHT 1985 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1985 American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c675t-b3bb30149aa060d6a48ed3022011b3fc97624e020091b302a23ce67027ca0de3</citedby><cites>FETCH-LOGICAL-c675t-b3bb30149aa060d6a48ed3022011b3fc97624e020091b302a23ce67027ca0de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1694554$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1694554$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9114926$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2581316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lipsky, Naomi Geller</creatorcontrib><creatorcontrib>Pagano, Richard E.</creatorcontrib><title>A Vital Stain for the Golgi Apparatus</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The Golgi complex, a membranous organelle with important functions in membrane traffic and macromolecular synthesis, has been stained in living cells with a fluorescent sphingolipid. Cells were first incubated with liposomes containing N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl sphingosine (C$_{6}$-NBD-ceramide), or with a bovine serum albumin complex of the fluorescent lipid, and then examined by fluorescence microscopy. An intensely fluorescent perinuclear structure was identified as the Golgi apparatus by its colocalization with known Golgi markers in fixed cells. C$_{6}$-NBD-ceramide was used to observe the morphology of the Golgi apparatus in living cells in the presence or absence of monensin or Colcemid, and during mitosis. In all cases, C$_{6}$-NBD-ceramide revealed a Golgi apparatus in the living cell that was identical to that obtained with conventional procedures that require fixation.</description><subject>3T3 cells</subject><subject>4-Chloro-7-nitrobenzofurazan - analogs & derivatives</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell lines</subject><subject>Cell research</subject><subject>Ceramides</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Cultured cells</subject><subject>Cytological research</subject><subject>Diverse techniques</subject><subject>Fibroblasts</subject><subject>Fibroblasts - ultrastructure</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Golgi apparatus</subject><subject>Golgi Apparatus - drug effects</subject><subject>Golgi Apparatus - ultrastructure</subject><subject>Hepatocytes</subject><subject>Humans</subject><subject>Lipids</subject><subject>Liposomes</subject><subject>Microscopy, Fluorescence</subject><subject>Mitosis</subject><subject>Molecular and cellular biology</subject><subject>Serum Albumin, Bovine</subject><subject>Staining and Labeling - methods</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0kFv2yAUB3A0berSbuddNsmHdTu0bh9gY_uYRVtaKVoOrXpFGD97VMTOAEvttx9VrLaTcog4IHg_Hkj8CflE4YJSJi69NthrvGB5STkVb8iMQpWnFQP-lswAuEhLKPL35Nj7e4BYq_gROZr4jJzOkzsTlE1ugjJ90g4uCX8wWQ62M8l8u1VOhdF_IO9aZT1-nOYTcvvr5-3iKl2tl9eL-SrVoshDWvO65kCzSikQ0AiVldhwYAworXmrq0KwDIHFZ8Q1MMW4RlEAK7SCBvkJ-bZru3XD3xF9kBvjNVqrehxGLwtBIXbLIzzbwU5ZlKZvh-CU7rBHp-zQY2vi9pwXoszyMurzPTqOBjdG7-Hf_-NRBHwInRq9l9c3vw-V67tD5Y_lgbJcrl7Ls31SD9ZihzJ-zGL9Wl_utHaD9w5buXVmo9yjpCCfoiSnKMkpG_HEl-k3xnqDzbN_qX-d6sprZVunem38M6toDAJ7Yp937N6Hwb3cKqoszzP-DzUR1MU</recordid><startdate>19850510</startdate><enddate>19850510</enddate><creator>Lipsky, Naomi Geller</creator><creator>Pagano, Richard E.</creator><general>The American Association for the Advancement of Science</general><general>American Association for the Advancement of Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>7X8</scope></search><sort><creationdate>19850510</creationdate><title>A Vital Stain for the Golgi Apparatus</title><author>Lipsky, Naomi Geller ; Pagano, Richard E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c675t-b3bb30149aa060d6a48ed3022011b3fc97624e020091b302a23ce67027ca0de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>3T3 cells</topic><topic>4-Chloro-7-nitrobenzofurazan - analogs & derivatives</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell lines</topic><topic>Cell research</topic><topic>Ceramides</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Cultured cells</topic><topic>Cytological research</topic><topic>Diverse techniques</topic><topic>Fibroblasts</topic><topic>Fibroblasts - ultrastructure</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Golgi apparatus</topic><topic>Golgi Apparatus - drug effects</topic><topic>Golgi Apparatus - ultrastructure</topic><topic>Hepatocytes</topic><topic>Humans</topic><topic>Lipids</topic><topic>Liposomes</topic><topic>Microscopy, Fluorescence</topic><topic>Mitosis</topic><topic>Molecular and cellular biology</topic><topic>Serum Albumin, Bovine</topic><topic>Staining and Labeling - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lipsky, Naomi Geller</creatorcontrib><creatorcontrib>Pagano, Richard E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: High School</collection><collection>Gale In Context: Biography</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Canada</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lipsky, Naomi Geller</au><au>Pagano, Richard E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Vital Stain for the Golgi Apparatus</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1985-05-10</date><risdate>1985</risdate><volume>228</volume><issue>4700</issue><spage>745</spage><epage>747</epage><pages>745-747</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>The Golgi complex, a membranous organelle with important functions in membrane traffic and macromolecular synthesis, has been stained in living cells with a fluorescent sphingolipid. Cells were first incubated with liposomes containing N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl sphingosine (C$_{6}$-NBD-ceramide), or with a bovine serum albumin complex of the fluorescent lipid, and then examined by fluorescence microscopy. An intensely fluorescent perinuclear structure was identified as the Golgi apparatus by its colocalization with known Golgi markers in fixed cells. C$_{6}$-NBD-ceramide was used to observe the morphology of the Golgi apparatus in living cells in the presence or absence of monensin or Colcemid, and during mitosis. In all cases, C$_{6}$-NBD-ceramide revealed a Golgi apparatus in the living cell that was identical to that obtained with conventional procedures that require fixation.</abstract><cop>Washington, DC</cop><pub>The American Association for the Advancement of Science</pub><pmid>2581316</pmid><doi>10.1126/science.2581316</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1985-05, Vol.228 (4700), p.745-747 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76100225 |
| source | MEDLINE; Science Magazine; JSTOR Archive Collection A-Z Listing |
| subjects | 3T3 cells 4-Chloro-7-nitrobenzofurazan - analogs & derivatives Animals Biological and medical sciences Cell lines Cell research Ceramides Cricetinae Cricetulus Cultured cells Cytological research Diverse techniques Fibroblasts Fibroblasts - ultrastructure Fluorescence Fundamental and applied biological sciences. Psychology Golgi apparatus Golgi Apparatus - drug effects Golgi Apparatus - ultrastructure Hepatocytes Humans Lipids Liposomes Microscopy, Fluorescence Mitosis Molecular and cellular biology Serum Albumin, Bovine Staining and Labeling - methods |
| title | A Vital Stain for the Golgi Apparatus |
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