A second polymorphic lens crystallin (LEN-2) in the mouse: genetic and biochemical analysis of LEN-1 and LEN-2
Two electrophoretic polymorphisms affecting lens crystallins, designated LEN-1 and LEN-2, have been discovered among inbred strains of mice. Analysis by isoelectric focusing demonstrated that both crystallins are monomeric proteins with isoelectric points at or above pH 7. Both proteins eluted in th...
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| Veröffentlicht in: | Biochemical genetics 1985-02, Vol.23 (1/2), p.181-189 |
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| container_title | Biochemical genetics |
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| creator | Skow, L.C Donner, M.E Popp, R.A Bailiff, E.G |
| description | Two electrophoretic polymorphisms affecting lens crystallins, designated LEN-1 and LEN-2, have been discovered among inbred strains of mice. Analysis by isoelectric focusing demonstrated that both crystallins are monomeric proteins with isoelectric points at or above pH 7. Both proteins eluted in the low molecular weight (LM) fraction upon Sephadex G-200 gel filtration but LEN-2 was shown to be larger than LEN-1 by G75SF gel filtration and denaturing gel electrophoresis. Linkage analysis demonstrated that the genes encoding LEN-1 and LEN-2 assort independently. Amino acid analysis of the allelic products of the two genes revealed that genetic variants of each respective crystallin were very similar in amino acid compositions but that LEN-1 and LEN-2 were dissimilar crystallins. |
| doi_str_mv | 10.1007/BF00499122 |
| format | Article |
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Analysis by isoelectric focusing demonstrated that both crystallins are monomeric proteins with isoelectric points at or above pH 7. Both proteins eluted in the low molecular weight (LM) fraction upon Sephadex G-200 gel filtration but LEN-2 was shown to be larger than LEN-1 by G75SF gel filtration and denaturing gel electrophoresis. Linkage analysis demonstrated that the genes encoding LEN-1 and LEN-2 assort independently. Amino acid analysis of the allelic products of the two genes revealed that genetic variants of each respective crystallin were very similar in amino acid compositions but that LEN-1 and LEN-2 were dissimilar crystallins.</description><identifier>ISSN: 0006-2928</identifier><identifier>EISSN: 1573-4927</identifier><identifier>DOI: 10.1007/BF00499122</identifier><identifier>PMID: 3994658</identifier><identifier>CODEN: BIGEBA</identifier><language>eng</language><publisher>New York, NY: Plenum Press</publisher><subject>Alleles ; Amino Acid Sequence ; Animals ; Biological and medical sciences ; Chromatography, Gel ; Classical genetics, quantitative genetics, hybrids ; Crystallins - analysis ; Crystallins - genetics ; Electrophoresis, Polyacrylamide Gel ; EYES ; Fundamental and applied biological sciences. Psychology ; GENETICA ; GENETICS ; Genetics of eukaryotes. Biological and molecular evolution ; GENETIQUE ; Genotype ; Isoelectric Focusing ; Mice ; Mice, Inbred Strains ; Molecular Weight ; OEIL ; OJOS ; Phenotype ; POLIMORFISMO ; POLYMORPHISM ; Polymorphism, Genetic ; POLYMORPHISME ; Vertebrata</subject><ispartof>Biochemical genetics, 1985-02, Vol.23 (1/2), p.181-189</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-d7bb7219dc1636ef3b490bd374d057a5b7b022c1db983e7769b55a3164bea83a3</citedby><cites>FETCH-LOGICAL-c330t-d7bb7219dc1636ef3b490bd374d057a5b7b022c1db983e7769b55a3164bea83a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9110646$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3994658$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Skow, L.C</creatorcontrib><creatorcontrib>Donner, M.E</creatorcontrib><creatorcontrib>Popp, R.A</creatorcontrib><creatorcontrib>Bailiff, E.G</creatorcontrib><title>A second polymorphic lens crystallin (LEN-2) in the mouse: genetic and biochemical analysis of LEN-1 and LEN-2</title><title>Biochemical genetics</title><addtitle>Biochem Genet</addtitle><description>Two electrophoretic polymorphisms affecting lens crystallins, designated LEN-1 and LEN-2, have been discovered among inbred strains of mice. Analysis by isoelectric focusing demonstrated that both crystallins are monomeric proteins with isoelectric points at or above pH 7. Both proteins eluted in the low molecular weight (LM) fraction upon Sephadex G-200 gel filtration but LEN-2 was shown to be larger than LEN-1 by G75SF gel filtration and denaturing gel electrophoresis. Linkage analysis demonstrated that the genes encoding LEN-1 and LEN-2 assort independently. Amino acid analysis of the allelic products of the two genes revealed that genetic variants of each respective crystallin were very similar in amino acid compositions but that LEN-1 and LEN-2 were dissimilar crystallins.</description><subject>Alleles</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Gel</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Crystallins - analysis</subject><subject>Crystallins - genetics</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>EYES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENETICA</subject><subject>GENETICS</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>GENETIQUE</subject><subject>Genotype</subject><subject>Isoelectric Focusing</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Molecular Weight</subject><subject>OEIL</subject><subject>OJOS</subject><subject>Phenotype</subject><subject>POLIMORFISMO</subject><subject>POLYMORPHISM</subject><subject>Polymorphism, Genetic</subject><subject>POLYMORPHISME</subject><subject>Vertebrata</subject><issn>0006-2928</issn><issn>1573-4927</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0M9LwzAUB_Agis4fF4-CkIOICtX8atJ4m8OpMPSgO5ckTbdK2sykO-y_N25jnpLH-7xH8gXgHKN7jJB4eBojxKTEhOyBAc4FzZgkYh8MEEI8I5IUR-A4xu9USsTYITikUjKeFwPQDWG0xncVXHi3an1YzBsDne0iNGEVe-Vc08GbyfN7Rm5huvZzC1u_jPYRzmxn-6RVmtaNN3PbNka5VCu3ik2EvoZ_g3gt1itOwUGtXLRn2_METMfPX6PXbPLx8jYaTjJDKeqzSmgtCJaVwZxyW1PNJNIVFaxCuVC5FhoRYnClZUGtEFzqPFcUc6atKqiiJ-B6s3cR_M_Sxr5sm2isc6qz6fGl4EjynMoE7zbQBB9jsHW5CE2rwqrEqPwLt_wPN-HL7dalbm21o9s0U_9q21cxBVEH1Zkm7pjEGHHGE7vYsFr5Us1CItPPguNCpN_-AtkyhoU</recordid><startdate>198502</startdate><enddate>198502</enddate><creator>Skow, L.C</creator><creator>Donner, M.E</creator><creator>Popp, R.A</creator><creator>Bailiff, E.G</creator><general>Plenum Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198502</creationdate><title>A second polymorphic lens crystallin (LEN-2) in the mouse: genetic and biochemical analysis of LEN-1 and LEN-2</title><author>Skow, L.C ; Donner, M.E ; Popp, R.A ; Bailiff, E.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-d7bb7219dc1636ef3b490bd374d057a5b7b022c1db983e7769b55a3164bea83a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Alleles</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Gel</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Crystallins - analysis</topic><topic>Crystallins - genetics</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>EYES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENETICA</topic><topic>GENETICS</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>GENETIQUE</topic><topic>Genotype</topic><topic>Isoelectric Focusing</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Molecular Weight</topic><topic>OEIL</topic><topic>OJOS</topic><topic>Phenotype</topic><topic>POLIMORFISMO</topic><topic>POLYMORPHISM</topic><topic>Polymorphism, Genetic</topic><topic>POLYMORPHISME</topic><topic>Vertebrata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Skow, L.C</creatorcontrib><creatorcontrib>Donner, M.E</creatorcontrib><creatorcontrib>Popp, R.A</creatorcontrib><creatorcontrib>Bailiff, E.G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Skow, L.C</au><au>Donner, M.E</au><au>Popp, R.A</au><au>Bailiff, E.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A second polymorphic lens crystallin (LEN-2) in the mouse: genetic and biochemical analysis of LEN-1 and LEN-2</atitle><jtitle>Biochemical genetics</jtitle><addtitle>Biochem Genet</addtitle><date>1985-02</date><risdate>1985</risdate><volume>23</volume><issue>1/2</issue><spage>181</spage><epage>189</epage><pages>181-189</pages><issn>0006-2928</issn><eissn>1573-4927</eissn><coden>BIGEBA</coden><abstract>Two electrophoretic polymorphisms affecting lens crystallins, designated LEN-1 and LEN-2, have been discovered among inbred strains of mice. Analysis by isoelectric focusing demonstrated that both crystallins are monomeric proteins with isoelectric points at or above pH 7. Both proteins eluted in the low molecular weight (LM) fraction upon Sephadex G-200 gel filtration but LEN-2 was shown to be larger than LEN-1 by G75SF gel filtration and denaturing gel electrophoresis. Linkage analysis demonstrated that the genes encoding LEN-1 and LEN-2 assort independently. Amino acid analysis of the allelic products of the two genes revealed that genetic variants of each respective crystallin were very similar in amino acid compositions but that LEN-1 and LEN-2 were dissimilar crystallins.</abstract><cop>New York, NY</cop><pub>Plenum Press</pub><pmid>3994658</pmid><doi>10.1007/BF00499122</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biochemical genetics, 1985-02, Vol.23 (1/2), p.181-189 |
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| subjects | Alleles Amino Acid Sequence Animals Biological and medical sciences Chromatography, Gel Classical genetics, quantitative genetics, hybrids Crystallins - analysis Crystallins - genetics Electrophoresis, Polyacrylamide Gel EYES Fundamental and applied biological sciences. Psychology GENETICA GENETICS Genetics of eukaryotes. Biological and molecular evolution GENETIQUE Genotype Isoelectric Focusing Mice Mice, Inbred Strains Molecular Weight OEIL OJOS Phenotype POLIMORFISMO POLYMORPHISM Polymorphism, Genetic POLYMORPHISME Vertebrata |
| title | A second polymorphic lens crystallin (LEN-2) in the mouse: genetic and biochemical analysis of LEN-1 and LEN-2 |
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