Inhibition of Adenosine-Mediated Responses in Isolated Hepatocytes by Depolarizing Concentrations of K
Isolated rat hepatocytes, incubated in a high K+ medium which depolarizes their plasma membrane, were used to investigate the response to adenosine. High K+ concentration blocked both the adenosine mediated increase of calcium influx and the increase in the rate of urea synthesis. It is concluded th...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1993-11, Vol.197 (1), p.229-234 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Guinzberg, R. Diazcruz, A. Uribe, S. Pina, E. |
| description | Isolated rat hepatocytes, incubated in a high K+ medium which depolarizes their plasma membrane, were used to investigate the response to adenosine. High K+ concentration blocked both the adenosine mediated increase of calcium influx and the increase in the rate of urea synthesis. It is concluded that a) adenosine stimulates calcium influx in hepatocytes probably through receptor-operated Ca2+ channels which are closed by depolarization of the plasma membrane, b) the higher cytosolic calcium concentration triggers a regulatory step that fully stimulates the rate in urea synthesis. |
| doi_str_mv | 10.1006/bbrc.1993.2465 |
| format | Article |
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High K+ concentration blocked both the adenosine mediated increase of calcium influx and the increase in the rate of urea synthesis. It is concluded that a) adenosine stimulates calcium influx in hepatocytes probably through receptor-operated Ca2+ channels which are closed by depolarization of the plasma membrane, b) the higher cytosolic calcium concentration triggers a regulatory step that fully stimulates the rate in urea synthesis.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.2465</identifier><identifier>PMID: 8250929</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adenosine - pharmacology ; Animals ; Biological and medical sciences ; Calcium - metabolism ; Calcium Channels - drug effects ; Cytosol - metabolism ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. 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High K+ concentration blocked both the adenosine mediated increase of calcium influx and the increase in the rate of urea synthesis. It is concluded that a) adenosine stimulates calcium influx in hepatocytes probably through receptor-operated Ca2+ channels which are closed by depolarization of the plasma membrane, b) the higher cytosolic calcium concentration triggers a regulatory step that fully stimulates the rate in urea synthesis.</description><subject>Adenosine - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Calcium Channels - drug effects</subject><subject>Cytosol - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Liver. Bile. Biliary tracts</subject><subject>Male</subject><subject>Potassium - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Urea - metabolism</subject><subject>Vertebrates: digestive system</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM9LHDEUx0Op2FV77a0wh-Jt1mR-5h1ltbqoFESht5Bk3rQps8k0b1bY_vXNuIs3Tw_e95PvCx_Gvgi-FJw3F8ZEuxQA5bKomvoDWwgOPC8Erz6yBU9EXoD4-YmdEP3hXIiqgWN2LIuaQwEL1q_9b2fc5ILPQp9ddugDOY_5A3ZOT9hlj0hj8ISUOZ-tKQyv21sc9RTsbkp7s8uucExBdP-c_5Wtgrfop6jnVppr787YUa8Hws-Hecqev18_rW7z-x8369XlfW7LRk45YAMcwBptyrbHghvetJUGDSiMkH0pwdoaZGLQCC3bqtWi6jtZc4QKi_KUne97xxj-bpEmtXFkcRi0x7Al1TYcqrpoErjcgzYGooi9GqPb6LhTgqtZrJrFqlmsmsWmB18PzVuzwe4NP5hM-bdDrsnqoY_aW0dvWCnLGl7vyj2GycKLw6jIOky-OhfRTqoL7r0f_AchMJUL</recordid><startdate>19931130</startdate><enddate>19931130</enddate><creator>Guinzberg, R.</creator><creator>Diazcruz, A.</creator><creator>Uribe, S.</creator><creator>Pina, E.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931130</creationdate><title>Inhibition of Adenosine-Mediated Responses in Isolated Hepatocytes by Depolarizing Concentrations of K</title><author>Guinzberg, R. ; Diazcruz, A. ; Uribe, S. ; Pina, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-9e69099cbab37fe20b0674a9a9e1b18f389cc598099eb1a8747a14fd850e94e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenosine - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Calcium Channels - drug effects</topic><topic>Cytosol - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Liver. Bile. Biliary tracts</topic><topic>Male</topic><topic>Potassium - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Urea - metabolism</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guinzberg, R.</creatorcontrib><creatorcontrib>Diazcruz, A.</creatorcontrib><creatorcontrib>Uribe, S.</creatorcontrib><creatorcontrib>Pina, E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guinzberg, R.</au><au>Diazcruz, A.</au><au>Uribe, S.</au><au>Pina, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of Adenosine-Mediated Responses in Isolated Hepatocytes by Depolarizing Concentrations of K</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-11-30</date><risdate>1993</risdate><volume>197</volume><issue>1</issue><spage>229</spage><epage>234</epage><pages>229-234</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Isolated rat hepatocytes, incubated in a high K+ medium which depolarizes their plasma membrane, were used to investigate the response to adenosine. High K+ concentration blocked both the adenosine mediated increase of calcium influx and the increase in the rate of urea synthesis. It is concluded that a) adenosine stimulates calcium influx in hepatocytes probably through receptor-operated Ca2+ channels which are closed by depolarization of the plasma membrane, b) the higher cytosolic calcium concentration triggers a regulatory step that fully stimulates the rate in urea synthesis.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8250929</pmid><doi>10.1006/bbrc.1993.2465</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1993-11, Vol.197 (1), p.229-234 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adenosine - pharmacology Animals Biological and medical sciences Calcium - metabolism Calcium Channels - drug effects Cytosol - metabolism Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Liver - cytology Liver - metabolism Liver. Bile. Biliary tracts Male Potassium - pharmacology Rats Rats, Wistar Urea - metabolism Vertebrates: digestive system |
| title | Inhibition of Adenosine-Mediated Responses in Isolated Hepatocytes by Depolarizing Concentrations of K |
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