Single-step monoclonal antibody affinity purification of human urogastrone from urine
The use of a monoclonal antibody (MAb) affinity column to purify epidermal growth factor/urogastrone from human urine in a single-step process is described. The MAb was raised against purified recombinant human urogastrone derived from the expression of a cloned synthetic urogastrone gene. The MAb w...
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| Veröffentlicht in: | Journal of immunological methods 1985-04, Vol.78 (2), p.211-216 |
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| container_title | Journal of immunological methods |
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| creator | Hissey, P.H. Thompson, K.J. Bawden, L. |
| description | The use of a monoclonal antibody (MAb) affinity column to purify epidermal growth factor/urogastrone from human urine in a single-step process is described. The MAb was raised against purified recombinant human urogastrone derived from the expression of a cloned synthetic urogastrone gene. The MAb was characterised and shown to cross-react fully with recombinant and native human urogastrones. The material eluted from the column was shown to have retained biological activity. When the eluted material was run on SDS/PAGE under reducing conditions an apparently homogeneous species of 5400 Da apparent molecular weight was seen. When examined on acid PAGE, 2 molecular species corresponding to β and γ urogastrone were observed. |
| doi_str_mv | 10.1016/0022-1759(85)90078-X |
| format | Article |
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The MAb was raised against purified recombinant human urogastrone derived from the expression of a cloned synthetic urogastrone gene. The MAb was characterised and shown to cross-react fully with recombinant and native human urogastrones. The material eluted from the column was shown to have retained biological activity. When the eluted material was run on SDS/PAGE under reducing conditions an apparently homogeneous species of 5400 Da apparent molecular weight was seen. When examined on acid PAGE, 2 molecular species corresponding to β and γ urogastrone were observed.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(85)90078-X</identifier><identifier>PMID: 3886801</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>affinity chromatography ; Antibodies, Monoclonal ; Biological and medical sciences ; Cross Reactions ; Epidermal Growth Factor - immunology ; Epidermal Growth Factor - isolation & purification ; Epidermal Growth Factor - urine ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunosorbent Techniques ; Molecular immunology ; Molecular Weight ; monoclonal antibody ; Techniques ; urogastrone</subject><ispartof>Journal of immunological methods, 1985-04, Vol.78 (2), p.211-216</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-2c395c8bac5e8f96771f000df5f012ebb555e2ed10d21b1986a474ac6af61c8d3</citedby><cites>FETCH-LOGICAL-c386t-2c395c8bac5e8f96771f000df5f012ebb555e2ed10d21b1986a474ac6af61c8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/002217598590078X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8537760$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3886801$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hissey, P.H.</creatorcontrib><creatorcontrib>Thompson, K.J.</creatorcontrib><creatorcontrib>Bawden, L.</creatorcontrib><title>Single-step monoclonal antibody affinity purification of human urogastrone from urine</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>The use of a monoclonal antibody (MAb) affinity column to purify epidermal growth factor/urogastrone from human urine in a single-step process is described. The MAb was raised against purified recombinant human urogastrone derived from the expression of a cloned synthetic urogastrone gene. The MAb was characterised and shown to cross-react fully with recombinant and native human urogastrones. The material eluted from the column was shown to have retained biological activity. When the eluted material was run on SDS/PAGE under reducing conditions an apparently homogeneous species of 5400 Da apparent molecular weight was seen. When examined on acid PAGE, 2 molecular species corresponding to β and γ urogastrone were observed.</description><subject>affinity chromatography</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Cross Reactions</subject><subject>Epidermal Growth Factor - immunology</subject><subject>Epidermal Growth Factor - isolation & purification</subject><subject>Epidermal Growth Factor - urine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunosorbent Techniques</subject><subject>Molecular immunology</subject><subject>Molecular Weight</subject><subject>monoclonal antibody</subject><subject>Techniques</subject><subject>urogastrone</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78A4UeRPRQnbSbj14EEb9gwYMK3kKaTjTSJmvSCvvv7brLHj0NzDzv8PIQckzhkgLlVwBFkVPBqnPJLioAIfP3LTKhUhS5qIBtk8kG2SP7KX0BAAUOu2S3lJJLoBPy9uL8R4t56nGedcEH0wav20z73tWhWWTaWuddv8jmQ3TWGd274LNgs8-h0z4bYvjQqY_BY2Zj6MaF83hIdqxuEx6t5wF5u797vX3MZ88PT7c3s9yUkvd5YcqKGVlrw1DaigtB7dixscwCLbCuGWNYYEOhKWhNK8n1VEy14dpyamRTHpCz1d95DN8Dpl51LhlsW-0xDEkJDhUtCzqC0xVoYkgpolXz6DodF4qCWtpUS1VqqUpJpv5sqvcxdrL-P9QdNpvQWt94P13fdTK6tVF749IGk6wUY4URu15hOLr4cRhVMg69wcZFNL1qgvu_xy-fm5JG</recordid><startdate>19850422</startdate><enddate>19850422</enddate><creator>Hissey, P.H.</creator><creator>Thompson, K.J.</creator><creator>Bawden, L.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850422</creationdate><title>Single-step monoclonal antibody affinity purification of human urogastrone from urine</title><author>Hissey, P.H. ; Thompson, K.J. ; Bawden, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-2c395c8bac5e8f96771f000df5f012ebb555e2ed10d21b1986a474ac6af61c8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>affinity chromatography</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Cross Reactions</topic><topic>Epidermal Growth Factor - immunology</topic><topic>Epidermal Growth Factor - isolation & purification</topic><topic>Epidermal Growth Factor - urine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunosorbent Techniques</topic><topic>Molecular immunology</topic><topic>Molecular Weight</topic><topic>monoclonal antibody</topic><topic>Techniques</topic><topic>urogastrone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hissey, P.H.</creatorcontrib><creatorcontrib>Thompson, K.J.</creatorcontrib><creatorcontrib>Bawden, L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hissey, P.H.</au><au>Thompson, K.J.</au><au>Bawden, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-step monoclonal antibody affinity purification of human urogastrone from urine</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1985-04-22</date><risdate>1985</risdate><volume>78</volume><issue>2</issue><spage>211</spage><epage>216</epage><pages>211-216</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>The use of a monoclonal antibody (MAb) affinity column to purify epidermal growth factor/urogastrone from human urine in a single-step process is described. The MAb was raised against purified recombinant human urogastrone derived from the expression of a cloned synthetic urogastrone gene. The MAb was characterised and shown to cross-react fully with recombinant and native human urogastrones. The material eluted from the column was shown to have retained biological activity. When the eluted material was run on SDS/PAGE under reducing conditions an apparently homogeneous species of 5400 Da apparent molecular weight was seen. When examined on acid PAGE, 2 molecular species corresponding to β and γ urogastrone were observed.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3886801</pmid><doi>10.1016/0022-1759(85)90078-X</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1985-04, Vol.78 (2), p.211-216 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | affinity chromatography Antibodies, Monoclonal Biological and medical sciences Cross Reactions Epidermal Growth Factor - immunology Epidermal Growth Factor - isolation & purification Epidermal Growth Factor - urine Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunosorbent Techniques Molecular immunology Molecular Weight monoclonal antibody Techniques urogastrone |
| title | Single-step monoclonal antibody affinity purification of human urogastrone from urine |
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