Human group II phospholipase A2 expressed in Trichoplusia ni larvae--isolation and kinetic properties of the enzyme

Human secreted synovial fluid/platelet-type group II phospholipase A2 (sPLA2) was expressed in Trichoplusia ni (cabbage looper) larvae and cultured Sf9 insect cells by infection with a recombinant baculovirus. Active sPLA2, with correct N-terminal proteolytic processing, was not secreted by Sf9 cell...

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Veröffentlicht in:	Protein expression and purification 1993-10, Vol.4 (5), p.490-498
Hauptverfasser:	Tremblay, N M, Kennedy, B P, Street, I P, Kaupp, W J, Laliberté, F, Weech, P K
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Cell Line Chromatography, Affinity DNA, Complementary - genetics Gene Expression Genetic Vectors Humans Kinetics Larva Mice Mice, Inbred BALB C Molecular Sequence Data Moths - classification Moths - growth & development Moths - metabolism Nucleopolyhedrovirus - genetics Phospholipases A - biosynthesis Phospholipases A - genetics Phospholipases A - immunology Phospholipases A - isolation & purification Phospholipases A2 Protein Engineering Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Species Specificity
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