Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product

The Escherichia coli gene for folylpolyglutamate synthetase-dihydrofolate synthetase was localized to plasmids pLC22-45, 24-31, and 28-44 of the Clarke-Carbon E. coli colony bank (Clarke, L., and Carbon, J. (1976) Cell 9, 91-99) by screening the bank by replica mating with an E. coli folC mutant. Th...

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Veröffentlicht in:	The Journal of biological chemistry 1985-05, Vol.260 (9), p.5625-5630
Hauptverfasser:	Bognar, A L, Osborne, C, Shane, B, Singer, S C, Ferone, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Cloning, Molecular DNA Restriction Enzymes - metabolism DNA, Bacterial - analysis Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Escherichia coli Proteins Gene Expression Regulation Kinetics Molecular Weight Multienzyme Complexes Peptide Synthases - genetics Plasmids
Online-Zugang:	Volltext
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