The basolateral membrane of rat enterocyte: Its purification from brush border contamination

Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 m m CaCl 2 to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Anal. Biochem.; (United States) 1985-02, Vol.144 (2), p.577-583
Hauptverfasser:	Orsenigo, M.N., Tosco, M., Esposito, G., Faelli, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	550301 - Cytology- Tracer Techniques ALDEHYDES ALKALI METAL COMPOUNDS ALKALI METAL ISOTOPES ALKALINE EARTH METAL COMPOUNDS ANIMALS Applied sciences BASIC BIOLOGICAL SCIENCES basolateral membrane vesicles basolateral membranes BETA DECAY RADIOISOTOPES BETA-PLUS DECAY RADIOISOTOPES BODY brush border membrane CaCl 2-precipitation method Calcium CALCIUM CHLORIDES CALCIUM COMPOUNDS CALCIUM HALIDES CARBOHYDRATES CELL CONSTITUENTS Cell Membrane - analysis Cell Membrane - enzymology CELL MEMBRANES CENTRIFUGATION CHLORIDES CHLORINE COMPOUNDS CYTOLOGICAL TECHNIQUES DIGESTIVE SYSTEM enterocytes Exact sciences and technology GASTROINTESTINAL TRACT GLUCOSE Glucose - metabolism HALIDES HALOGEN COMPOUNDS HEXOSES INTESTINES ISOTOPE APPLICATIONS ISOTOPES l-glucose uptake LIGHT NUCLEI Male MAMMALS MEMBRANES Microvilli - analysis MONOSACCHARIDES NUCLEI ODD-ODD NUCLEI ORGANIC COMPOUNDS ORGANS Other techniques and industries Percoll-gradient centrifugation PERMEABILITY RADIOISOTOPES RATS Rats, Inbred Strains RODENTS SACCHARIDES SEPARATION PROCESSES Sodium - metabolism SODIUM 22 SODIUM COMPOUNDS SODIUM ISOTOPES Sodium Radioisotopes sodium uptake Sodium-Potassium-Exchanging ATPase - metabolism TRACER TECHNIQUES VERTEBRATES YEARS LIVING RADIOISOTOPES
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 m m CaCl 2 to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low l-glucose permeability across basolateral membrane vesicles together with ATP-dependent sodium uptake was observed.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(85)90156-3