Oxonol dyes as monitors of membrane potential: The effect of viruses and toxins on the plasma membrane potential of animal cells in monolayer culture and in suspension
Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potential either of K+ (in the presence of valinomycin) or of H+ (...
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| Veröffentlicht in: | Journal of cellular physiology 1985-06, Vol.123 (3), p.326-336 |
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| container_end_page | 336 |
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| container_issue | 3 |
| container_start_page | 326 |
| container_title | Journal of cellular physiology |
| container_volume | 123 |
| creator | Bashford, C. Lindsay Alder, Glenn M. Gray, Michael A. Micklem, Kingsley J. Taylor, C. Christopher Turek, Paul J. Pasternak, Charles A. |
| description | Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potential either of K+ (in the presence of valinomycin) or of H+ (in the presence of carbonyl cyanide p‐trifluoromethoxyphenylhydrazone; FCCP); both classes of dye gave similar values of plasma membrane potential, in the range −40 to −90 mV for different cell types. Addition of haemolytic Sendai virus or Staphylococcus aureus α‐toxin depolarizes cells and causes them to leak monovalent cations; these effects are antagonized by extracellular Ca2+. Cells infected with vesicular stomatitis or Semliki Forest virus become depolarized during an infectious cycle; infection with other viruses was without affect on plasma membrane potential. |
| doi_str_mv | 10.1002/jcp.1041230306 |
| format | Article |
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Lindsay ; Alder, Glenn M. ; Gray, Michael A. ; Micklem, Kingsley J. ; Taylor, C. Christopher ; Turek, Paul J. ; Pasternak, Charles A.</creator><creatorcontrib>Bashford, C. Lindsay ; Alder, Glenn M. ; Gray, Michael A. ; Micklem, Kingsley J. ; Taylor, C. Christopher ; Turek, Paul J. ; Pasternak, Charles A.</creatorcontrib><description>Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potential either of K+ (in the presence of valinomycin) or of H+ (in the presence of carbonyl cyanide p‐trifluoromethoxyphenylhydrazone; FCCP); both classes of dye gave similar values of plasma membrane potential, in the range −40 to −90 mV for different cell types. Addition of haemolytic Sendai virus or Staphylococcus aureus α‐toxin depolarizes cells and causes them to leak monovalent cations; these effects are antagonized by extracellular Ca2+. Cells infected with vesicular stomatitis or Semliki Forest virus become depolarized during an infectious cycle; infection with other viruses was without affect on plasma membrane potential.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.1041230306</identifier><identifier>PMID: 3988810</identifier><identifier>CODEN: JCLLAX</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>animal cells ; Animals ; Benzothiazoles ; Biological and medical sciences ; Carbocyanines ; Cell Membrane - drug effects ; Cell Membrane - physiology ; dyes ; Fundamental and applied biological sciences. Psychology ; Isoxazoles ; membrane potential ; Membrane Potentials - drug effects ; Mice ; Mice, Inbred Strains ; Microbiology ; Oxazoles ; plasma membranes ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; toxins ; Toxins, Biological - pharmacology ; Virology ; Virus Diseases - physiopathology ; viruses</subject><ispartof>Journal of cellular physiology, 1985-06, Vol.123 (3), p.326-336</ispartof><rights>Copyright © 1985 Wiley‐Liss, Inc.</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4386-2ed3fd49fc5341d32e8b3e9efdaad21d2f6afacf4d4c5bfdc695d58b77e94da13</citedby><cites>FETCH-LOGICAL-c4386-2ed3fd49fc5341d32e8b3e9efdaad21d2f6afacf4d4c5bfdc695d58b77e94da13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.1041230306$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.1041230306$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8461739$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3988810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bashford, C. Lindsay</creatorcontrib><creatorcontrib>Alder, Glenn M.</creatorcontrib><creatorcontrib>Gray, Michael A.</creatorcontrib><creatorcontrib>Micklem, Kingsley J.</creatorcontrib><creatorcontrib>Taylor, C. Christopher</creatorcontrib><creatorcontrib>Turek, Paul J.</creatorcontrib><creatorcontrib>Pasternak, Charles A.</creatorcontrib><title>Oxonol dyes as monitors of membrane potential: The effect of viruses and toxins on the plasma membrane potential of animal cells in monolayer culture and in suspension</title><title>Journal of cellular physiology</title><addtitle>J. Cell. Physiol</addtitle><description>Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potential either of K+ (in the presence of valinomycin) or of H+ (in the presence of carbonyl cyanide p‐trifluoromethoxyphenylhydrazone; FCCP); both classes of dye gave similar values of plasma membrane potential, in the range −40 to −90 mV for different cell types. Addition of haemolytic Sendai virus or Staphylococcus aureus α‐toxin depolarizes cells and causes them to leak monovalent cations; these effects are antagonized by extracellular Ca2+. Cells infected with vesicular stomatitis or Semliki Forest virus become depolarized during an infectious cycle; infection with other viruses was without affect on plasma membrane potential.</description><subject>animal cells</subject><subject>Animals</subject><subject>Benzothiazoles</subject><subject>Biological and medical sciences</subject><subject>Carbocyanines</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - physiology</subject><subject>dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isoxazoles</subject><subject>membrane potential</subject><subject>Membrane Potentials - drug effects</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Microbiology</subject><subject>Oxazoles</subject><subject>plasma membranes</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>toxins</subject><subject>Toxins, Biological - pharmacology</subject><subject>Virology</subject><subject>Virus Diseases - physiopathology</subject><subject>viruses</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxRtR1tnVqzchB_HWa9JJ_4k3GXRXWVzBlREvIZ1UMGs6aZNunflEfk3TzjAiCHtKQf3eq6q8onhC8DnBuHpxq8ZcMFJRTHFzr1gRzNuSNXV1v1hlgJS8ZuRhcZrSLcaYc0pPihPKu64jeFX8ut4GHxzSO0hIJjQEb6cQEwoGDTD0UXpAY5jAT1a6l-jmKyAwBtS0ED9snNMi9BpNYWt91nk0ZWZ0Mg3yPxaLTHo75EqBcwlZvwwNTu4gIjW7aY7wxzA30pxG8MkG_6h4YKRL8PjwnhWf3ry-WV-WV9cXb9evrkrFaNeUFWhqNONG1ZQRTSvoegocjJZSV0RXppFGKsM0U3VvtGp4reuub1vgTEtCz4rne98xhu8zpEkMNi2L5iPCnETb4K6pa3wnSBijVYsXx_M9qGJIKYIRY8znx50gWCwRihyh-BthFjw9OM_9APqIHzLL_WeHvkxKOpP_V9l0xDrWkJbyjPE99tM62N0xVLxbf_hnhXKvtWmC7VEr4zfRtLStxeb9hdiQqv68-fhFbOhvNJnInA</recordid><startdate>198506</startdate><enddate>198506</enddate><creator>Bashford, C. Lindsay</creator><creator>Alder, Glenn M.</creator><creator>Gray, Michael A.</creator><creator>Micklem, Kingsley J.</creator><creator>Taylor, C. Christopher</creator><creator>Turek, Paul J.</creator><creator>Pasternak, Charles A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198506</creationdate><title>Oxonol dyes as monitors of membrane potential: The effect of viruses and toxins on the plasma membrane potential of animal cells in monolayer culture and in suspension</title><author>Bashford, C. Lindsay ; Alder, Glenn M. ; Gray, Michael A. ; Micklem, Kingsley J. ; Taylor, C. 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The optical signals were calibrated by using diffusion potential either of K+ (in the presence of valinomycin) or of H+ (in the presence of carbonyl cyanide p‐trifluoromethoxyphenylhydrazone; FCCP); both classes of dye gave similar values of plasma membrane potential, in the range −40 to −90 mV for different cell types. Addition of haemolytic Sendai virus or Staphylococcus aureus α‐toxin depolarizes cells and causes them to leak monovalent cations; these effects are antagonized by extracellular Ca2+. Cells infected with vesicular stomatitis or Semliki Forest virus become depolarized during an infectious cycle; infection with other viruses was without affect on plasma membrane potential.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3988810</pmid><doi>10.1002/jcp.1041230306</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of cellular physiology, 1985-06, Vol.123 (3), p.326-336 |
| issn | 0021-9541 1097-4652 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | animal cells Animals Benzothiazoles Biological and medical sciences Carbocyanines Cell Membrane - drug effects Cell Membrane - physiology dyes Fundamental and applied biological sciences. Psychology Isoxazoles membrane potential Membrane Potentials - drug effects Mice Mice, Inbred Strains Microbiology Oxazoles plasma membranes Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains toxins Toxins, Biological - pharmacology Virology Virus Diseases - physiopathology viruses |
| title | Oxonol dyes as monitors of membrane potential: The effect of viruses and toxins on the plasma membrane potential of animal cells in monolayer culture and in suspension |
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