Analysis of Femtomole Concentrations of α-Ketoisocaproic Acid in Brain Tissue by Precolumn Fluorescence Derivatization with 4,5-Dimethoxy-1,2-diaminobenzene

An extremely sensitive fluorimetric HPLC method used to determine in vivo cerebral α-ketoisocaproic acid (KIC) concentration with less than 2 g of tissue (e.g., a single rat brain, ∼1.8 g) is reported. Removal of unwanted lipids and amino acids and isolation of α-ketoacids in an optimal derivatizati...

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Veröffentlicht in:	Analytical biochemistry 1993-08, Vol.213 (1), p.23-28
Hauptverfasser:	Keen, R.E., Nissenson, C.H., Barrio, J.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Brain Chemistry Carbon Radioisotopes Chromatography, High Pressure Liquid - methods Fluorescent Dyes Fluorometry Fundamental and applied biological sciences. Psychology Keto Acids - analysis Keto Acids - blood Keto Acids - isolation & purification Leucine - metabolism Male Microchemistry Phenylenediamines - chemistry Proteins Rats Rats, Sprague-Dawley Reproducibility of Results Sensitivity and Specificity Swine
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