Characterization of New Monoclonal Antibodies Identifying Avian T Lymphocyte Antigens
Six monoclonal antibodies (mAb) were produced to identify and characterize surface antigens of chicken T cells. Determination of their reactivity with different lymphatic cells using immunofluorescence analysis demonstrates that mAb KH8, NA6, PD4 and TH8 stained 32–43% blood lymphocytes, 72–77% thym...
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| Veröffentlicht in: | Immunobiology (1979) 1993-08, Vol.188 (4), p.415-429 |
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| description | Six monoclonal antibodies (mAb) were produced to identify and characterize surface antigens of chicken T cells. Determination of their reactivity with different lymphatic cells using immunofluorescence analysis demonstrates that mAb KH8, NA6, PD4 and TH8 stained 32–43% blood lymphocytes, 72–77% thymocytes and 19–27% spleen cells, mAb OC5 approximately 99% thymocytes and 55% blood and spleen lymphocytes each, and mAb OC2 36% blood lymphocytes, 79% thymocytes and 62% spleen cells. The KH8, NA6, PD4 and TH8 antibodies immunoprecipitated from lysates of surface-labeled chicken thymocytes a polypeptide of M
r 60,000 under non-reducing conditions and the OC5 antibody a glycoprotein of M
r 68,000 under reducing conditions. MAb OC2 precipitated a single polypeptide of M
r 40,000 under both conditions. The mAb KH8, NA6, PD4, TH8 and OC2 inhibited Con A-induced proliferative responses of blood T cells
in vitro. However, sepharose-bound or soluble OC5 antibody was able to increase DNA synthesis significantly.
These results indicate that (a) the mAb KH8, NA6, PD4 and TH8 identify the avian homologue of the mammalian CD4 molecule, (b) the mAb OC2 detects the avian CD2 antigen, and (c) the mAb OC5 recognizes the putative avian CD5 homologue. |
| doi_str_mv | 10.1016/S0171-2985(11)80224-X |
| format | Article |
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r 60,000 under non-reducing conditions and the OC5 antibody a glycoprotein of M
r 68,000 under reducing conditions. MAb OC2 precipitated a single polypeptide of M
r 40,000 under both conditions. The mAb KH8, NA6, PD4, TH8 and OC2 inhibited Con A-induced proliferative responses of blood T cells
in vitro. However, sepharose-bound or soluble OC5 antibody was able to increase DNA synthesis significantly.
These results indicate that (a) the mAb KH8, NA6, PD4 and TH8 identify the avian homologue of the mammalian CD4 molecule, (b) the mAb OC2 detects the avian CD2 antigen, and (c) the mAb OC5 recognizes the putative avian CD5 homologue.</description><identifier>ISSN: 0171-2985</identifier><identifier>EISSN: 1878-3279</identifier><identifier>DOI: 10.1016/S0171-2985(11)80224-X</identifier><identifier>PMID: 8244446</identifier><identifier>CODEN: IMMND4</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>ANALYTICAL METHODS ; Animals ; Antibodies, immunoglobulins ; Antibodies, Monoclonal - pharmacology ; Antibody Specificity ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; ANTIGEN ANTIBODY REACTIONS ; ANTIGENE ; ANTIGENOS ; ANTIGENS ; Antigens, Differentiation, T-Lymphocyte - chemistry ; Antigens, Differentiation, T-Lymphocyte - isolation & purification ; Antilymphocyte Serum ; Biological and medical sciences ; CELL DIFFERENTIATION ; Chickens - immunology ; DIFERENCIACION CELULAR ; DIFFERENCIATION CELLULAIRE ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; GALLINA PONEDORA ; GLICOPROTEINAS ; GLYCOPROTEINE ; GLYCOPROTEINS ; IDENTIFICACION ; IDENTIFICATION ; IMMUNITE ; IMMUNITY ; IMMUNOELECTROPHORESE ; IMMUNOELECTROPHORESIS ; IMMUNOFLUORESCENCE ; INMUNIDAD ; INMUNOELECTROFORESIS ; INMUNOFLUORESCENCIA ; LAYER CHICKENS ; LINFOCITOS ; LYMPHOCYTE ; Lymphocyte Activation ; LYMPHOCYTES ; Mice ; Mice, Inbred BALB C ; Molecular immunology ; MOLECULAR WEIGHT ; MONOCLONAL ANTIBODIES ; PEPTIDE ; PEPTIDES ; PEPTIDOS ; PESO MOLECULAR ; POIDS MOLECULAIRE ; POULE PONDEUSE ; REACCION ANTIGENO-ANTICUERPO ; REACTION ANTIGENE ANTICORPS ; Spleen - immunology ; T-Lymphocytes - immunology ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; Thymus Gland - immunology ; Tissue Distribution</subject><ispartof>Immunobiology (1979), 1993-08, Vol.188 (4), p.415-429</ispartof><rights>1993 Gustav Fischer Verlag · Stuttgart · New York</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-99446d566229e4e7589ba7cb34a5d6832ca84a5a61e5151697d9eef2a90dae3a3</citedby><cites>FETCH-LOGICAL-c408t-99446d566229e4e7589ba7cb34a5d6832ca84a5a61e5151697d9eef2a90dae3a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0171-2985(11)80224-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3759310$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8244446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Knabel, Michael</creatorcontrib><creatorcontrib>Cihak, Josef</creatorcontrib><creatorcontrib>Lösch, Uli</creatorcontrib><creatorcontrib>Muenchen Univ. (Germany). Inst. fuer Tierphysiologie</creatorcontrib><title>Characterization of New Monoclonal Antibodies Identifying Avian T Lymphocyte Antigens</title><title>Immunobiology (1979)</title><addtitle>Immunobiology</addtitle><description>Six monoclonal antibodies (mAb) were produced to identify and characterize surface antigens of chicken T cells. Determination of their reactivity with different lymphatic cells using immunofluorescence analysis demonstrates that mAb KH8, NA6, PD4 and TH8 stained 32–43% blood lymphocytes, 72–77% thymocytes and 19–27% spleen cells, mAb OC5 approximately 99% thymocytes and 55% blood and spleen lymphocytes each, and mAb OC2 36% blood lymphocytes, 79% thymocytes and 62% spleen cells. The KH8, NA6, PD4 and TH8 antibodies immunoprecipitated from lysates of surface-labeled chicken thymocytes a polypeptide of M
r 60,000 under non-reducing conditions and the OC5 antibody a glycoprotein of M
r 68,000 under reducing conditions. MAb OC2 precipitated a single polypeptide of M
r 40,000 under both conditions. The mAb KH8, NA6, PD4, TH8 and OC2 inhibited Con A-induced proliferative responses of blood T cells
in vitro. However, sepharose-bound or soluble OC5 antibody was able to increase DNA synthesis significantly.
These results indicate that (a) the mAb KH8, NA6, PD4 and TH8 identify the avian homologue of the mammalian CD4 molecule, (b) the mAb OC2 detects the avian CD2 antigen, and (c) the mAb OC5 recognizes the putative avian CD5 homologue.</description><subject>ANALYTICAL METHODS</subject><subject>Animals</subject><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Antibody Specificity</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>ANTIGEN ANTIBODY REACTIONS</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>ANTIGENS</subject><subject>Antigens, Differentiation, T-Lymphocyte - chemistry</subject><subject>Antigens, Differentiation, T-Lymphocyte - isolation & purification</subject><subject>Antilymphocyte Serum</subject><subject>Biological and medical sciences</subject><subject>CELL DIFFERENTIATION</subject><subject>Chickens - immunology</subject><subject>DIFERENCIACION CELULAR</subject><subject>DIFFERENCIATION CELLULAIRE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>GALLINA PONEDORA</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>GLYCOPROTEINS</subject><subject>IDENTIFICACION</subject><subject>IDENTIFICATION</subject><subject>IMMUNITE</subject><subject>IMMUNITY</subject><subject>IMMUNOELECTROPHORESE</subject><subject>IMMUNOELECTROPHORESIS</subject><subject>IMMUNOFLUORESCENCE</subject><subject>INMUNIDAD</subject><subject>INMUNOELECTROFORESIS</subject><subject>INMUNOFLUORESCENCIA</subject><subject>LAYER CHICKENS</subject><subject>LINFOCITOS</subject><subject>LYMPHOCYTE</subject><subject>Lymphocyte Activation</subject><subject>LYMPHOCYTES</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular immunology</subject><subject>MOLECULAR WEIGHT</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>PEPTIDE</subject><subject>PEPTIDES</subject><subject>PEPTIDOS</subject><subject>PESO MOLECULAR</subject><subject>POIDS MOLECULAIRE</subject><subject>POULE PONDEUSE</subject><subject>REACCION ANTIGENO-ANTICUERPO</subject><subject>REACTION ANTIGENE ANTICORPS</subject><subject>Spleen - immunology</subject><subject>T-Lymphocytes - immunology</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>Thymus Gland - immunology</subject><subject>Tissue Distribution</subject><issn>0171-2985</issn><issn>1878-3279</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9vFCEYh4nR1LX6BUyazMEYexgFZhjgZDZrrU1WPdgmvZF34Z0tZha2MFuzfnrZP9mrXID8nhd-eQi5YPQjo6z79IsyyWqulfjA2KWinLf1_TMyYUqquuFSPyeTE_KSvMr5N6VMc6nOyJnibVndhNzNHiCBHTH5vzD6GKrYVz_wT_U9hmiHGGCopmH0i-g85urGYbn0Wx-W1fTJQ6huq_l2tX6IdjvinlxiyK_Jix6GjG-O-zm5-3p1O_tWz39e38ym89q2VI211qWDE13HucYWpVB6AdIumhaE61TDLahyhI6hYIJ1WjqN2HPQ1AE20JyT94d31yk-bjCPZuWzxWGAgHGTjeyooqJlBRQH0KaYc8LerJNfQdoaRs1Op9nrNDtXhjGz12nuy9zF8YPNYoXuNHX0V_J3xxyyhaFPEKzPJ6yRQjeMFuztAeshGlimgny50m1Dqdh1-3wIsZh68phMth6DRecT2tG46P_T8h9BvJmu</recordid><startdate>19930801</startdate><enddate>19930801</enddate><creator>Knabel, Michael</creator><creator>Cihak, Josef</creator><creator>Lösch, Uli</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930801</creationdate><title>Characterization of New Monoclonal Antibodies Identifying Avian T Lymphocyte Antigens</title><author>Knabel, Michael ; Cihak, Josef ; Lösch, Uli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-99446d566229e4e7589ba7cb34a5d6832ca84a5a61e5151697d9eef2a90dae3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>ANALYTICAL METHODS</topic><topic>Animals</topic><topic>Antibodies, immunoglobulins</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>Antibody Specificity</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>ANTIGEN ANTIBODY REACTIONS</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>ANTIGENS</topic><topic>Antigens, Differentiation, T-Lymphocyte - chemistry</topic><topic>Antigens, Differentiation, T-Lymphocyte - isolation & purification</topic><topic>Antilymphocyte Serum</topic><topic>Biological and medical sciences</topic><topic>CELL DIFFERENTIATION</topic><topic>Chickens - immunology</topic><topic>DIFERENCIACION CELULAR</topic><topic>DIFFERENCIATION CELLULAIRE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>GALLINA PONEDORA</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>GLYCOPROTEINS</topic><topic>IDENTIFICACION</topic><topic>IDENTIFICATION</topic><topic>IMMUNITE</topic><topic>IMMUNITY</topic><topic>IMMUNOELECTROPHORESE</topic><topic>IMMUNOELECTROPHORESIS</topic><topic>IMMUNOFLUORESCENCE</topic><topic>INMUNIDAD</topic><topic>INMUNOELECTROFORESIS</topic><topic>INMUNOFLUORESCENCIA</topic><topic>LAYER CHICKENS</topic><topic>LINFOCITOS</topic><topic>LYMPHOCYTE</topic><topic>Lymphocyte Activation</topic><topic>LYMPHOCYTES</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular immunology</topic><topic>MOLECULAR WEIGHT</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>PEPTIDE</topic><topic>PEPTIDES</topic><topic>PEPTIDOS</topic><topic>PESO MOLECULAR</topic><topic>POIDS MOLECULAIRE</topic><topic>POULE PONDEUSE</topic><topic>REACCION ANTIGENO-ANTICUERPO</topic><topic>REACTION ANTIGENE ANTICORPS</topic><topic>Spleen - immunology</topic><topic>T-Lymphocytes - immunology</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>Thymus Gland - immunology</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Knabel, Michael</creatorcontrib><creatorcontrib>Cihak, Josef</creatorcontrib><creatorcontrib>Lösch, Uli</creatorcontrib><creatorcontrib>Muenchen Univ. (Germany). Inst. fuer Tierphysiologie</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Immunobiology (1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Knabel, Michael</au><au>Cihak, Josef</au><au>Lösch, Uli</au><aucorp>Muenchen Univ. (Germany). Inst. fuer Tierphysiologie</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of New Monoclonal Antibodies Identifying Avian T Lymphocyte Antigens</atitle><jtitle>Immunobiology (1979)</jtitle><addtitle>Immunobiology</addtitle><date>1993-08-01</date><risdate>1993</risdate><volume>188</volume><issue>4</issue><spage>415</spage><epage>429</epage><pages>415-429</pages><issn>0171-2985</issn><eissn>1878-3279</eissn><coden>IMMND4</coden><abstract>Six monoclonal antibodies (mAb) were produced to identify and characterize surface antigens of chicken T cells. Determination of their reactivity with different lymphatic cells using immunofluorescence analysis demonstrates that mAb KH8, NA6, PD4 and TH8 stained 32–43% blood lymphocytes, 72–77% thymocytes and 19–27% spleen cells, mAb OC5 approximately 99% thymocytes and 55% blood and spleen lymphocytes each, and mAb OC2 36% blood lymphocytes, 79% thymocytes and 62% spleen cells. The KH8, NA6, PD4 and TH8 antibodies immunoprecipitated from lysates of surface-labeled chicken thymocytes a polypeptide of M
r 60,000 under non-reducing conditions and the OC5 antibody a glycoprotein of M
r 68,000 under reducing conditions. MAb OC2 precipitated a single polypeptide of M
r 40,000 under both conditions. The mAb KH8, NA6, PD4, TH8 and OC2 inhibited Con A-induced proliferative responses of blood T cells
in vitro. However, sepharose-bound or soluble OC5 antibody was able to increase DNA synthesis significantly.
These results indicate that (a) the mAb KH8, NA6, PD4 and TH8 identify the avian homologue of the mammalian CD4 molecule, (b) the mAb OC2 detects the avian CD2 antigen, and (c) the mAb OC5 recognizes the putative avian CD5 homologue.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>8244446</pmid><doi>10.1016/S0171-2985(11)80224-X</doi><tpages>15</tpages></addata></record> |
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| ispartof | Immunobiology (1979), 1993-08, Vol.188 (4), p.415-429 |
| issn | 0171-2985 1878-3279 |
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| subjects | ANALYTICAL METHODS Animals Antibodies, immunoglobulins Antibodies, Monoclonal - pharmacology Antibody Specificity ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES ANTIGEN ANTIBODY REACTIONS ANTIGENE ANTIGENOS ANTIGENS Antigens, Differentiation, T-Lymphocyte - chemistry Antigens, Differentiation, T-Lymphocyte - isolation & purification Antilymphocyte Serum Biological and medical sciences CELL DIFFERENTIATION Chickens - immunology DIFERENCIACION CELULAR DIFFERENCIATION CELLULAIRE Fundamental and applied biological sciences. Psychology Fundamental immunology GALLINA PONEDORA GLICOPROTEINAS GLYCOPROTEINE GLYCOPROTEINS IDENTIFICACION IDENTIFICATION IMMUNITE IMMUNITY IMMUNOELECTROPHORESE IMMUNOELECTROPHORESIS IMMUNOFLUORESCENCE INMUNIDAD INMUNOELECTROFORESIS INMUNOFLUORESCENCIA LAYER CHICKENS LINFOCITOS LYMPHOCYTE Lymphocyte Activation LYMPHOCYTES Mice Mice, Inbred BALB C Molecular immunology MOLECULAR WEIGHT MONOCLONAL ANTIBODIES PEPTIDE PEPTIDES PEPTIDOS PESO MOLECULAR POIDS MOLECULAIRE POULE PONDEUSE REACCION ANTIGENO-ANTICUERPO REACTION ANTIGENE ANTICORPS Spleen - immunology T-Lymphocytes - immunology TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS Thymus Gland - immunology Tissue Distribution |
| title | Characterization of New Monoclonal Antibodies Identifying Avian T Lymphocyte Antigens |
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