Papillomavirus infection of the lower genital tract: Detection of viral DNA in gynecological swabs
A total of 311 smears from the lower genital tract were examined by the filter in situ hybridization method to identify human papillomavirus (HPV) DNA. Of these 311 smears, 229 came from clinically and cytologically negative patients and served as a control group. In this group HPV‐DNA was detected...
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| Veröffentlicht in: | International journal of cancer 1985-04, Vol.35 (4), p.443-448 |
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| creator | Schneider, Achim Kraus, Herbert Schuhmann, Roland Gissmann, Lutz |
| description | A total of 311 smears from the lower genital tract were examined by the filter in situ hybridization method to identify human papillomavirus (HPV) DNA.
Of these 311 smears, 229 came from clinically and cytologically negative patients and served as a control group. In this group HPV‐DNA was detected in 5 cases (2.2%).
Of 82 cytologically positive cases (25 confirmed by histology) 56 (68%) contained HPV‐DNA. A high prevalence of HPV 6/11 and absence of HPV 16/18 was found in cases with cytological signs of permissive HPV infection. In mild and moderate dysplasia all viruses occurred at almost the same frequency. In severe dysplasia/carcinoma in situ HPV 16/18 was found 5 times more frequently than HPV 6/11. HPV 16/18 was identified in all 4 invasive cancer cases.
Cervical irrigation of colposcopically suspect areas was performed in 15 cytologically and HPV‐DNA positive cases using the hydrodynamic filtration method. In 12 cases only the cells obtained from the colposcopically positive areas contained HPV‐DNA.
The sensitivity and reproducibility of the filter in situ hybridization was shown by: (1) comparing the results obtained by HPV‐DNA hybridization using Southern blot analysis of tumor biopsies; (2) analysing the correlation of cytologic diagnosis and presence of HPV‐DNA in follow‐up examinations, and (3) diagnosing presence or absence of HPV‐DNA in parallel filters from the same patients. |
| doi_str_mv | 10.1002/ijc.2910350405 |
| format | Article |
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Of these 311 smears, 229 came from clinically and cytologically negative patients and served as a control group. In this group HPV‐DNA was detected in 5 cases (2.2%).
Of 82 cytologically positive cases (25 confirmed by histology) 56 (68%) contained HPV‐DNA. A high prevalence of HPV 6/11 and absence of HPV 16/18 was found in cases with cytological signs of permissive HPV infection. In mild and moderate dysplasia all viruses occurred at almost the same frequency. In severe dysplasia/carcinoma in situ HPV 16/18 was found 5 times more frequently than HPV 6/11. HPV 16/18 was identified in all 4 invasive cancer cases.
Cervical irrigation of colposcopically suspect areas was performed in 15 cytologically and HPV‐DNA positive cases using the hydrodynamic filtration method. In 12 cases only the cells obtained from the colposcopically positive areas contained HPV‐DNA.
The sensitivity and reproducibility of the filter in situ hybridization was shown by: (1) comparing the results obtained by HPV‐DNA hybridization using Southern blot analysis of tumor biopsies; (2) analysing the correlation of cytologic diagnosis and presence of HPV‐DNA in follow‐up examinations, and (3) diagnosing presence or absence of HPV‐DNA in parallel filters from the same patients.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.2910350405</identifier><identifier>PMID: 2985509</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Autoradiography ; Biological and medical sciences ; Cell Separation ; Colposcopy ; DNA, Viral - analysis ; Female ; Genital Neoplasms, Female - diagnosis ; Genital Neoplasms, Female - microbiology ; Genital Neoplasms, Female - pathology ; Human viral diseases ; Humans ; Infectious diseases ; Medical sciences ; Nucleic Acid Hybridization ; Papillomaviridae - metabolism ; Precancerous Conditions - diagnosis ; Tumor Virus Infections - diagnosis ; Tumor Virus Infections - microbiology ; Tumor Virus Infections - pathology ; Uterine Cervical Neoplasms - diagnosis ; Vaginal Smears ; Viral diseases ; Viral diseases of the genital and urinary system</subject><ispartof>International journal of cancer, 1985-04, Vol.35 (4), p.443-448</ispartof><rights>Copyright © 1985 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4005-a2816f8624a8c83b52840aabd15fc53a963a282728795d82cd5e08ae08d609d3</citedby><cites>FETCH-LOGICAL-c4005-a2816f8624a8c83b52840aabd15fc53a963a282728795d82cd5e08ae08d609d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.2910350405$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.2910350405$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8472300$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2985509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schneider, Achim</creatorcontrib><creatorcontrib>Kraus, Herbert</creatorcontrib><creatorcontrib>Schuhmann, Roland</creatorcontrib><creatorcontrib>Gissmann, Lutz</creatorcontrib><title>Papillomavirus infection of the lower genital tract: Detection of viral DNA in gynecological swabs</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>A total of 311 smears from the lower genital tract were examined by the filter in situ hybridization method to identify human papillomavirus (HPV) DNA.
Of these 311 smears, 229 came from clinically and cytologically negative patients and served as a control group. In this group HPV‐DNA was detected in 5 cases (2.2%).
Of 82 cytologically positive cases (25 confirmed by histology) 56 (68%) contained HPV‐DNA. A high prevalence of HPV 6/11 and absence of HPV 16/18 was found in cases with cytological signs of permissive HPV infection. In mild and moderate dysplasia all viruses occurred at almost the same frequency. In severe dysplasia/carcinoma in situ HPV 16/18 was found 5 times more frequently than HPV 6/11. HPV 16/18 was identified in all 4 invasive cancer cases.
Cervical irrigation of colposcopically suspect areas was performed in 15 cytologically and HPV‐DNA positive cases using the hydrodynamic filtration method. In 12 cases only the cells obtained from the colposcopically positive areas contained HPV‐DNA.
The sensitivity and reproducibility of the filter in situ hybridization was shown by: (1) comparing the results obtained by HPV‐DNA hybridization using Southern blot analysis of tumor biopsies; (2) analysing the correlation of cytologic diagnosis and presence of HPV‐DNA in follow‐up examinations, and (3) diagnosing presence or absence of HPV‐DNA in parallel filters from the same patients.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Cell Separation</subject><subject>Colposcopy</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>Genital Neoplasms, Female - diagnosis</subject><subject>Genital Neoplasms, Female - microbiology</subject><subject>Genital Neoplasms, Female - pathology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Nucleic Acid Hybridization</subject><subject>Papillomaviridae - metabolism</subject><subject>Precancerous Conditions - diagnosis</subject><subject>Tumor Virus Infections - diagnosis</subject><subject>Tumor Virus Infections - microbiology</subject><subject>Tumor Virus Infections - pathology</subject><subject>Uterine Cervical Neoplasms - diagnosis</subject><subject>Vaginal Smears</subject><subject>Viral diseases</subject><subject>Viral diseases of the genital and urinary system</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9PwjAYhhujQUSv3kx2MN6GX7t1a70R8AeGqAfuS9d1WFJWbIeE_94SCHjj0DTp-7zf1zwI3WLoYwDyqOeyTziGhEIK9Ax1MfA8BoLpOeoGAOIcJ9kluvJ-DoBxwDqoQzijFHgXlV9iqY2xC_Gr3cpHuqmVbLVtIltH7beKjF0rF81Uo1thotYJ2T5FI9UeqVAMyehjEMrRbNMoaY2daRke_VqU_hpd1MJ4dbO_e2j68jwdvsWTz9fxcDCJZQpAY0EYzmqWkVQwyZKSEpaCEGWFaS1pIniWBITkhOWcVozIiipgIpwqA14lPfSwG7t09melfFsstJfKGNEou_JFnkHOSc5PgjjdruFpAPs7UDrrvVN1sXR6IdymwFBs5RdBfnGUHwp3-8mrcqGqA763HfL7fS580FM70UjtDxhLc5IABIzvsLU2anNiaTF-H_77wh8Wq5x1</recordid><startdate>19850415</startdate><enddate>19850415</enddate><creator>Schneider, Achim</creator><creator>Kraus, Herbert</creator><creator>Schuhmann, Roland</creator><creator>Gissmann, Lutz</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19850415</creationdate><title>Papillomavirus infection of the lower genital tract: Detection of viral DNA in gynecological swabs</title><author>Schneider, Achim ; Kraus, Herbert ; Schuhmann, Roland ; Gissmann, Lutz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4005-a2816f8624a8c83b52840aabd15fc53a963a282728795d82cd5e08ae08d609d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Cell Separation</topic><topic>Colposcopy</topic><topic>DNA, Viral - analysis</topic><topic>Female</topic><topic>Genital Neoplasms, Female - diagnosis</topic><topic>Genital Neoplasms, Female - microbiology</topic><topic>Genital Neoplasms, Female - pathology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Nucleic Acid Hybridization</topic><topic>Papillomaviridae - metabolism</topic><topic>Precancerous Conditions - diagnosis</topic><topic>Tumor Virus Infections - diagnosis</topic><topic>Tumor Virus Infections - microbiology</topic><topic>Tumor Virus Infections - pathology</topic><topic>Uterine Cervical Neoplasms - diagnosis</topic><topic>Vaginal Smears</topic><topic>Viral diseases</topic><topic>Viral diseases of the genital and urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schneider, Achim</creatorcontrib><creatorcontrib>Kraus, Herbert</creatorcontrib><creatorcontrib>Schuhmann, Roland</creatorcontrib><creatorcontrib>Gissmann, Lutz</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schneider, Achim</au><au>Kraus, Herbert</au><au>Schuhmann, Roland</au><au>Gissmann, Lutz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Papillomavirus infection of the lower genital tract: Detection of viral DNA in gynecological swabs</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>1985-04-15</date><risdate>1985</risdate><volume>35</volume><issue>4</issue><spage>443</spage><epage>448</epage><pages>443-448</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>A total of 311 smears from the lower genital tract were examined by the filter in situ hybridization method to identify human papillomavirus (HPV) DNA.
Of these 311 smears, 229 came from clinically and cytologically negative patients and served as a control group. In this group HPV‐DNA was detected in 5 cases (2.2%).
Of 82 cytologically positive cases (25 confirmed by histology) 56 (68%) contained HPV‐DNA. A high prevalence of HPV 6/11 and absence of HPV 16/18 was found in cases with cytological signs of permissive HPV infection. In mild and moderate dysplasia all viruses occurred at almost the same frequency. In severe dysplasia/carcinoma in situ HPV 16/18 was found 5 times more frequently than HPV 6/11. HPV 16/18 was identified in all 4 invasive cancer cases.
Cervical irrigation of colposcopically suspect areas was performed in 15 cytologically and HPV‐DNA positive cases using the hydrodynamic filtration method. In 12 cases only the cells obtained from the colposcopically positive areas contained HPV‐DNA.
The sensitivity and reproducibility of the filter in situ hybridization was shown by: (1) comparing the results obtained by HPV‐DNA hybridization using Southern blot analysis of tumor biopsies; (2) analysing the correlation of cytologic diagnosis and presence of HPV‐DNA in follow‐up examinations, and (3) diagnosing presence or absence of HPV‐DNA in parallel filters from the same patients.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2985509</pmid><doi>10.1002/ijc.2910350405</doi><tpages>6</tpages></addata></record> |
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| ispartof | International journal of cancer, 1985-04, Vol.35 (4), p.443-448 |
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| subjects | Animals Autoradiography Biological and medical sciences Cell Separation Colposcopy DNA, Viral - analysis Female Genital Neoplasms, Female - diagnosis Genital Neoplasms, Female - microbiology Genital Neoplasms, Female - pathology Human viral diseases Humans Infectious diseases Medical sciences Nucleic Acid Hybridization Papillomaviridae - metabolism Precancerous Conditions - diagnosis Tumor Virus Infections - diagnosis Tumor Virus Infections - microbiology Tumor Virus Infections - pathology Uterine Cervical Neoplasms - diagnosis Vaginal Smears Viral diseases Viral diseases of the genital and urinary system |
| title | Papillomavirus infection of the lower genital tract: Detection of viral DNA in gynecological swabs |
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