Production of a monoclonal antibody directed against the nerve growth factor receptor from sympathetic membranes
Spleen cells from BALB/c mice immunized with a plasma membrane‐enriched fraction from rabbit sympathetic ganglia were fused with the mouse myeloma NS1. A hybrid clone was obtained that produced monoclonal antibody directed against the receptor for nerve growth factor (NGF). The antibody, identified...
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| Veröffentlicht in: | Journal of cellular biochemistry 1985, Vol.27 (2), p.121-132 |
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| container_end_page | 132 |
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| container_issue | 2 |
| container_start_page | 121 |
| container_title | Journal of cellular biochemistry |
| container_volume | 27 |
| creator | Morgan, Claudia J. Bradshaw, Ralph A. |
| description | Spleen cells from BALB/c mice immunized with a plasma membrane‐enriched fraction from rabbit sympathetic ganglia were fused with the mouse myeloma NS1. A hybrid clone was obtained that produced monoclonal antibody directed against the receptor for nerve growth factor (NGF). The antibody, identified as IgG, was able to immunoprecipitate solubilized NGF receptor in the presence or absence of bound NGF. The antibody bound specifically to sympathetic membranes with high affinity but did not affect the binding of 125I‐NGF to its receptor in sympathetic or sensory neurons or PC12 cells. |
| doi_str_mv | 10.1002/jcb.240270206 |
| format | Article |
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A hybrid clone was obtained that produced monoclonal antibody directed against the receptor for nerve growth factor (NGF). The antibody, identified as IgG, was able to immunoprecipitate solubilized NGF receptor in the presence or absence of bound NGF. The antibody bound specifically to sympathetic membranes with high affinity but did not affect the binding of 125I‐NGF to its receptor in sympathetic or sensory neurons or PC12 cells.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.240270206</identifier><identifier>PMID: 2985634</identifier><identifier>CODEN: JCEBD5</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Monoclonal - isolation & purification ; Biological and medical sciences ; Cell receptors ; Cell structures and functions ; Cloning, Molecular ; Fundamental and applied biological sciences. Psychology ; ganglia ; Ganglia, Sympathetic - metabolism ; growth factor receptors ; Mice ; Molecular and cellular biology ; monoclonal antibodies ; nerve growth factor ; Nerve Growth Factors - metabolism ; Rabbits ; receptors ; Receptors, Cell Surface - immunology ; Receptors, Cell Surface - metabolism ; Receptors, Nerve Growth Factor ; sympathetic nervous system</subject><ispartof>Journal of cellular biochemistry, 1985, Vol.27 (2), p.121-132</ispartof><rights>Copyright © 1985 Alan R. Liss, Inc.</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4346-dcd8f4b03c6dc55cebe61a3cb18f4939e307522c017e2354123685702b16b6813</citedby><cites>FETCH-LOGICAL-c4346-dcd8f4b03c6dc55cebe61a3cb18f4939e307522c017e2354123685702b16b6813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.240270206$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.240270206$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9255546$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2985634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morgan, Claudia J.</creatorcontrib><creatorcontrib>Bradshaw, Ralph A.</creatorcontrib><title>Production of a monoclonal antibody directed against the nerve growth factor receptor from sympathetic membranes</title><title>Journal of cellular biochemistry</title><addtitle>J. Cell. Biochem</addtitle><description>Spleen cells from BALB/c mice immunized with a plasma membrane‐enriched fraction from rabbit sympathetic ganglia were fused with the mouse myeloma NS1. A hybrid clone was obtained that produced monoclonal antibody directed against the receptor for nerve growth factor (NGF). The antibody, identified as IgG, was able to immunoprecipitate solubilized NGF receptor in the presence or absence of bound NGF. The antibody bound specifically to sympathetic membranes with high affinity but did not affect the binding of 125I‐NGF to its receptor in sympathetic or sensory neurons or PC12 cells.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Cloning, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>ganglia</subject><subject>Ganglia, Sympathetic - metabolism</subject><subject>growth factor receptors</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>monoclonal antibodies</subject><subject>nerve growth factor</subject><subject>Nerve Growth Factors - metabolism</subject><subject>Rabbits</subject><subject>receptors</subject><subject>Receptors, Cell Surface - immunology</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Receptors, Nerve Growth Factor</subject><subject>sympathetic nervous system</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAUxC0EKtvCkSOSD4hbyvN3fIQVbEEt9ADiaDmO07okcWp7afe_J6uNVpzgZMvv53mjGYReETgnAPTdnWvOKQeqgIJ8glYEtKq45PwpWoFiUFFG6HN0mvMdAGjN6Ak6oboWkvEVmq5TbLeuhDji2GGLhzhG18fR9tiOJTSx3eE2JO-Kb7G9sWHMBZdbj0effnt8k-JDucWddSUmPGN-2l-6FAecd8NkZ7QEhwc_NMmOPr9AzzrbZ_9yOc_Qj08fv68vqstvm8_r95eV44zLqnVt3fEGmJOtE8L5xktimWvI_KyZ9gyUoNQBUZ4ywQllshZzBg2RjawJO0NvD7pTivdbn4sZQna-72cTcZuNkqA0Bf1fkHCotdB7xeoAuhRzTr4zUwqDTTtDwOyrMHMV5ljFzL9ehLfN4NsjvWQ_z98sc5ud7bs5HhfyEdNUCMH3MuqAPYTe7_6903xZf_jbwGI45OIfjz9t-mWkYkqYn1835uL66oqt2cYA-wN3G7Eb</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Morgan, Claudia J.</creator><creator>Bradshaw, Ralph A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SQ</scope><scope>7T5</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1985</creationdate><title>Production of a monoclonal antibody directed against the nerve growth factor receptor from sympathetic membranes</title><author>Morgan, Claudia J. ; Bradshaw, Ralph A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4346-dcd8f4b03c6dc55cebe61a3cb18f4939e307522c017e2354123685702b16b6813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Monoclonal - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>ganglia</topic><topic>Ganglia, Sympathetic - metabolism</topic><topic>growth factor receptors</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>monoclonal antibodies</topic><topic>nerve growth factor</topic><topic>Nerve Growth Factors - metabolism</topic><topic>Rabbits</topic><topic>receptors</topic><topic>Receptors, Cell Surface - immunology</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Receptors, Nerve Growth Factor</topic><topic>sympathetic nervous system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morgan, Claudia J.</creatorcontrib><creatorcontrib>Bradshaw, Ralph A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Endocrinology Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morgan, Claudia J.</au><au>Bradshaw, Ralph A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of a monoclonal antibody directed against the nerve growth factor receptor from sympathetic membranes</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J. Cell. Biochem</addtitle><date>1985</date><risdate>1985</risdate><volume>27</volume><issue>2</issue><spage>121</spage><epage>132</epage><pages>121-132</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><coden>JCEBD5</coden><abstract>Spleen cells from BALB/c mice immunized with a plasma membrane‐enriched fraction from rabbit sympathetic ganglia were fused with the mouse myeloma NS1. A hybrid clone was obtained that produced monoclonal antibody directed against the receptor for nerve growth factor (NGF). The antibody, identified as IgG, was able to immunoprecipitate solubilized NGF receptor in the presence or absence of bound NGF. The antibody bound specifically to sympathetic membranes with high affinity but did not affect the binding of 125I‐NGF to its receptor in sympathetic or sensory neurons or PC12 cells.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2985634</pmid><doi>10.1002/jcb.240270206</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0730-2312 |
| ispartof | Journal of cellular biochemistry, 1985, Vol.27 (2), p.121-132 |
| issn | 0730-2312 1097-4644 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76079209 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Biological and medical sciences Cell receptors Cell structures and functions Cloning, Molecular Fundamental and applied biological sciences. Psychology ganglia Ganglia, Sympathetic - metabolism growth factor receptors Mice Molecular and cellular biology monoclonal antibodies nerve growth factor Nerve Growth Factors - metabolism Rabbits receptors Receptors, Cell Surface - immunology Receptors, Cell Surface - metabolism Receptors, Nerve Growth Factor sympathetic nervous system |
| title | Production of a monoclonal antibody directed against the nerve growth factor receptor from sympathetic membranes |
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