Identification of the Heat Shock Protein of Neurospora crassa Corresponding to the Stress-Inducible Peroxidase

Heat shock and other stress treatments, resulting in thermotolerance in Neurospora crossa cells, stimulate the induction of a peroxidase at a high level. The putative gene encoding this heat shock-induced peroxidase (hspp) has been cloned, using a cDNA clone of the manganese peroxidase of Phanerochaete chrysosporium, as a probe. Northern blot analysis of total RNA from heat-shocked cell showed the stress-dependent accumulation of a ∼10 kb transcript. The identity of the HSP, corresponding to the heat-induced peroxidase, was established using hybrid-arrested in vitro translation.