The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes
Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelenght is obtained with v...
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| Veröffentlicht in: | FEBS letters 1985-05, Vol.184 (1), p.96-99 |
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| creator | van Walraven, H.S. Krab, K. Hagendoorn, M.J.M. Kraayenhof, R. |
| description | Carotenoids present in lipids extracted from the cyanobacterium
Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelenght is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed. |
| doi_str_mv | 10.1016/0014-5793(85)80661-X |
| format | Article |
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Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelenght is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(85)80661-X</identifier><identifier>PMID: 3157600</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adenosine Triphosphatases ; Artificial membranes and reconstituted systems ; Biological and medical sciences ; Carotenoid shift ; Carotenoids ; Cyanobacteria - metabolism ; Diffusion potential ; Fundamental and applied biological sciences. Psychology ; Isoxazoles ; liposomes ; Membrane physicochemistry ; Membrane potential ; Membrane Potentials ; Molecular biophysics ; Oxazoles ; Oxonol VI ; Potassium - metabolism ; Proteolipids ; Proteoliposome</subject><ispartof>FEBS letters, 1985-05, Vol.184 (1), p.96-99</ispartof><rights>1985</rights><rights>FEBS Letters 184 (1985) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592X-fb95e873fde46b027e7a44fdb220817d18436df69ff42221378661e5b0278ef93</citedby><cites>FETCH-LOGICAL-c592X-fb95e873fde46b027e7a44fdb220817d18436df69ff42221378661e5b0278ef93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001457938580661X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8487720$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3157600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van Walraven, H.S.</creatorcontrib><creatorcontrib>Krab, K.</creatorcontrib><creatorcontrib>Hagendoorn, M.J.M.</creatorcontrib><creatorcontrib>Kraayenhof, R.</creatorcontrib><title>The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Carotenoids present in lipids extracted from the cyanobacterium
Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelenght is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed.</description><subject>Adenosine Triphosphatases</subject><subject>Artificial membranes and reconstituted systems</subject><subject>Biological and medical sciences</subject><subject>Carotenoid shift</subject><subject>Carotenoids</subject><subject>Cyanobacteria - metabolism</subject><subject>Diffusion potential</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isoxazoles</subject><subject>liposomes</subject><subject>Membrane physicochemistry</subject><subject>Membrane potential</subject><subject>Membrane Potentials</subject><subject>Molecular biophysics</subject><subject>Oxazoles</subject><subject>Oxonol VI</subject><subject>Potassium - metabolism</subject><subject>Proteolipids</subject><subject>Proteoliposome</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1rFDEYgIModa3-A4UcpOhhNMnkay4FLV1bKHipsreQmbzByMxkTWat_fcm3WWP1lPI-z7v14PQa0o-UELlR0Iob4Tq2ndavNdEStpsnqAV1aptWi71U7Q6Is_Ri5x_kvLXtDtBJy0VShKyQpvbH4B3GXD0eLApLjDH4DK2s8PxT5zjiL9fY5vxNsUeMvYx4QmmPtkZ8LbiS7AjDnMFFohj2MYcJ8gv0TNvxwyvDu8p-ra-vL24am6-frm--HTTDKJjm8b3nYCysXfAZU-YAmU5965njGiqHNW8lc7LznvOGKOt0uVQEBXV4Lv2FJ3t-5b5v3aQFzOFPMA4lgXjLptypqJSsEdBylkrpdYF5HtwSDHnBN5sU5hsujeUmGreVK2majVamAfzZlPK3hz67_oJ3LHooLrk3x7yNg929MXgEPIR01wrxSq23mN3YYT7_xpt1pefWU3UuBYP0brP-b4RFPu_AySThwDzAC4kGBbjYvj3QX8B0ZmyZA</recordid><startdate>19850506</startdate><enddate>19850506</enddate><creator>van Walraven, H.S.</creator><creator>Krab, K.</creator><creator>Hagendoorn, M.J.M.</creator><creator>Kraayenhof, R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19850506</creationdate><title>The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes</title><author>van Walraven, H.S. ; Krab, K. ; Hagendoorn, M.J.M. ; Kraayenhof, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592X-fb95e873fde46b027e7a44fdb220817d18436df69ff42221378661e5b0278ef93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Adenosine Triphosphatases</topic><topic>Artificial membranes and reconstituted systems</topic><topic>Biological and medical sciences</topic><topic>Carotenoid shift</topic><topic>Carotenoids</topic><topic>Cyanobacteria - metabolism</topic><topic>Diffusion potential</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isoxazoles</topic><topic>liposomes</topic><topic>Membrane physicochemistry</topic><topic>Membrane potential</topic><topic>Membrane Potentials</topic><topic>Molecular biophysics</topic><topic>Oxazoles</topic><topic>Oxonol VI</topic><topic>Potassium - metabolism</topic><topic>Proteolipids</topic><topic>Proteoliposome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Walraven, H.S.</creatorcontrib><creatorcontrib>Krab, K.</creatorcontrib><creatorcontrib>Hagendoorn, M.J.M.</creatorcontrib><creatorcontrib>Kraayenhof, R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Walraven, H.S.</au><au>Krab, K.</au><au>Hagendoorn, M.J.M.</au><au>Kraayenhof, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1985-05-06</date><risdate>1985</risdate><volume>184</volume><issue>1</issue><spage>96</spage><epage>99</epage><pages>96-99</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Carotenoids present in lipids extracted from the cyanobacterium
Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelenght is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3157600</pmid><doi>10.1016/0014-5793(85)80661-X</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1985-05, Vol.184 (1), p.96-99 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adenosine Triphosphatases Artificial membranes and reconstituted systems Biological and medical sciences Carotenoid shift Carotenoids Cyanobacteria - metabolism Diffusion potential Fundamental and applied biological sciences. Psychology Isoxazoles liposomes Membrane physicochemistry Membrane potential Membrane Potentials Molecular biophysics Oxazoles Oxonol VI Potassium - metabolism Proteolipids Proteoliposome |
| title | The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes |
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