Isolation and Point of Action of a Factor from Escherichia coli Required to Reconstruct Translation

To study the mechanism of translation we have attempted to reconstruct the process from purified components. Protein synthesis was programmed by the RNAs of wild-type or amber mutants of bacteriophages f2 or MS2. Translation programmed by MS2 or f2am3 RNA does not occur using ribosomes, precharged a...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1985-03, Vol.82 (6), p.1648-1652
Hauptverfasser:	Ganoza, M. Clelia, Cunningham, Christina, Green, Robert M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Bacterial Proteins - biosynthesis Bacterial Proteins - isolation & purification Bacterial Proteins - pharmacology Bacteriophages Biochemistry Biological and medical sciences Capsid proteins Enzymes Escherichia coli Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Gels Molecular and cellular biology Molecular genetics Protein Biosynthesis Protein synthesis Ribosomes Ribosomes - metabolism RNA RNA, Transfer - metabolism Transfer RNA Translation. Translation factors. Protein processing
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