A novel, sensitive radioimmunoprecipitation assay for the detection of antibodies to human immunodeficiency virus-type 2
A radioimmunoprecipitation assay that provides an alternative to the Western blot assay was developed for characterizing antibodies against human immunodeficiency virus type-2 (HIV-2). The assay is based on radioiodination of antigen using Bolton-Hunter reagent. The antigen consists of a soluble pre...
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| Veröffentlicht in: | Journal of virological methods 1993-09, Vol.44 (1), p.1-10 |
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| container_title | Journal of virological methods |
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| creator | Selvam, Mouna Panneer Mayner, Ronald E. Buck, Sheila M. Epstein, Jay S. |
| description | A radioimmunoprecipitation assay that provides an alternative to the Western blot assay was developed for characterizing antibodies against human immunodeficiency virus type-2 (HIV-2). The assay is based on radioiodination of antigen using Bolton-Hunter reagent. The antigen consists of a soluble preparation of the NIH-Z (HIV-2) strain of 1000X purified virus spiked with purified recombinant HIV-2 gp105. Radiolabeled proteins were immunoprecipitated by immune human sera, even at the early stages of seroconversion. This new assay provides a simple method for characterizing and titrating antibodies against HIV-2. The method is more sensitive, and is more efficient than Western blotting. The labeled viral proteins are well suited for biochemical studies. |
| doi_str_mv | 10.1016/0166-0934(93)90002-9 |
| format | Article |
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The assay is based on radioiodination of antigen using Bolton-Hunter reagent. The antigen consists of a soluble preparation of the NIH-Z (HIV-2) strain of 1000X purified virus spiked with purified recombinant HIV-2 gp105. Radiolabeled proteins were immunoprecipitated by immune human sera, even at the early stages of seroconversion. This new assay provides a simple method for characterizing and titrating antibodies against HIV-2. The method is more sensitive, and is more efficient than Western blotting. The labeled viral proteins are well suited for biochemical studies.</description><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Glycosylation</subject><subject>HIV Antibodies - blood</subject><subject>HIV-2</subject><subject>HIV-2 - immunology</subject><subject>HIV-2 - isolation & purification</subject><subject>human immunodeficiency virus 2</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Medical sciences</subject><subject>Radioimmunoprecipitation Assay</subject><subject>RIPA</subject><subject>Sensitivity and Specificity</subject><subject>Succinimides</subject><subject>Western blot</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoYzv6DxSyEFGwNI-qPDYDw-ALBtzoOqSTGyZSVSmTVGP_e9MPeqmLELj3y7k35yD0kpIPlFDxsR3REc37t5q_04QQ1ulHaEOV1K2s-sdoc0Geomel_GrMIDm_QleKMckk36A_t3hOOxjf4wJziTXuAGfrY4rTtM5pyeDiEqutMc3YlmL3OKSM6wNgDxXcsZ4CtnON2-QjFFwTflgnO-OThIcQXYTZ7fEu5rV0db8AZs_Rk2DHAi_O9zX6-fnTj7uv3f33L9_ubu8711NZO04GGIKwW6XIllvBvNDesV5xqokYrOuFkgMJhBPWc01VkDZw6RkXrA9M82v05qS75PR7hVLNFIuDcbQzpLUYKYhQVIv_glRIpdqUBvYn0OVUSoZglhwnm_eGEnNIxhxsNwfbjebmmIw5LPLqrL9uJ_CXR-coWv_1uW-Ls2PIdnaxXDAu238ladjNCYNm2i5CNuXoLvjYsqrGp_jvPf4C7Oaqeg</recordid><startdate>19930901</startdate><enddate>19930901</enddate><creator>Selvam, Mouna Panneer</creator><creator>Mayner, Ronald E.</creator><creator>Buck, Sheila M.</creator><creator>Epstein, Jay S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19930901</creationdate><title>A novel, sensitive radioimmunoprecipitation assay for the detection of antibodies to human immunodeficiency virus-type 2</title><author>Selvam, Mouna Panneer ; Mayner, Ronald E. ; Buck, Sheila M. ; Epstein, Jay S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-305e5f6ab880b3a62d69dc248319065ac468750f030243918f7af37d23624f293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Glycosylation</topic><topic>HIV Antibodies - blood</topic><topic>HIV-2</topic><topic>HIV-2 - immunology</topic><topic>HIV-2 - isolation & purification</topic><topic>human immunodeficiency virus 2</topic><topic>Humans</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Medical sciences</topic><topic>Radioimmunoprecipitation Assay</topic><topic>RIPA</topic><topic>Sensitivity and Specificity</topic><topic>Succinimides</topic><topic>Western blot</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Selvam, Mouna Panneer</creatorcontrib><creatorcontrib>Mayner, Ronald E.</creatorcontrib><creatorcontrib>Buck, Sheila M.</creatorcontrib><creatorcontrib>Epstein, Jay S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Selvam, Mouna Panneer</au><au>Mayner, Ronald E.</au><au>Buck, Sheila M.</au><au>Epstein, Jay S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel, sensitive radioimmunoprecipitation assay for the detection of antibodies to human immunodeficiency virus-type 2</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1993-09-01</date><risdate>1993</risdate><volume>44</volume><issue>1</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>A radioimmunoprecipitation assay that provides an alternative to the Western blot assay was developed for characterizing antibodies against human immunodeficiency virus type-2 (HIV-2). 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| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1993-09, Vol.44 (1), p.1-10 |
| issn | 0166-0934 1879-0984 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | AIDS/HIV Biological and medical sciences Blotting, Western Glycosylation HIV Antibodies - blood HIV-2 HIV-2 - immunology HIV-2 - isolation & purification human immunodeficiency virus 2 Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Medical sciences Radioimmunoprecipitation Assay RIPA Sensitivity and Specificity Succinimides Western blot |
| title | A novel, sensitive radioimmunoprecipitation assay for the detection of antibodies to human immunodeficiency virus-type 2 |
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