In vitro effects of psychoactive and non-psychoactive cannabinoids on immature rat sertoli cell function

We have examined the effects of psychoactive and non-psychoactive cannabinoids on isolated immature rat Sertoli cells cultured in either serum-free or serum-containing media. Lactate accumulation by Sertoli cells in serum-free control cultures was 10 fold greater than the values obtained in control...

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Veröffentlicht in:Life sciences (1973) 1993, Vol.53 (18), p.1429-1437
Hauptverfasser: Newton, Sean C., Murphy, Laura L., Bartke, Andrzej
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container_issue 18
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container_title Life sciences (1973)
container_volume 53
creator Newton, Sean C.
Murphy, Laura L.
Bartke, Andrzej
description We have examined the effects of psychoactive and non-psychoactive cannabinoids on isolated immature rat Sertoli cells cultured in either serum-free or serum-containing media. Lactate accumulation by Sertoli cells in serum-free control cultures was 10 fold greater than the values obtained in control cultures exposed to serum. Under either culture condition, 3.1 μg/ml of delta-9-tetrahydrocannabinol (THC) or cannabinol (CBN) stimulated lactate secretion above control levels. Cannabidiol (CBD) stimulated lactate secretion in serum-containing but not in serum-free media. Using serum-free culture conditions, we next studied the in vitro effects of combinations of THC and either epinephrine or FSH on lactate and transferrin secretion by immature rat Sertoli cells. Co-incubation of 0.1 μM epinephrine with 0.8 or 3.1 μg/ml THC significantly stimulated lactate secretion when compared to epinephrine or THC alone, while only the high THC dose increased transferrin secretion. Moreover, co-incubation of FSH (1 μg/ml) with THC (0.8 or 3.1 μg/ml), significantly stimulated both lactate and transferrin production by immature rat Sertoli cells. These results add to the growing evidence that cannabinoids can exert direct effects on Sertoli cell function and modulate their responses to physiological stimuli.
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Lactate accumulation by Sertoli cells in serum-free control cultures was 10 fold greater than the values obtained in control cultures exposed to serum. Under either culture condition, 3.1 μg/ml of delta-9-tetrahydrocannabinol (THC) or cannabinol (CBN) stimulated lactate secretion above control levels. Cannabidiol (CBD) stimulated lactate secretion in serum-containing but not in serum-free media. Using serum-free culture conditions, we next studied the in vitro effects of combinations of THC and either epinephrine or FSH on lactate and transferrin secretion by immature rat Sertoli cells. Co-incubation of 0.1 μM epinephrine with 0.8 or 3.1 μg/ml THC significantly stimulated lactate secretion when compared to epinephrine or THC alone, while only the high THC dose increased transferrin secretion. Moreover, co-incubation of FSH (1 μg/ml) with THC (0.8 or 3.1 μg/ml), significantly stimulated both lactate and transferrin production by immature rat Sertoli cells. 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Lactate accumulation by Sertoli cells in serum-free control cultures was 10 fold greater than the values obtained in control cultures exposed to serum. Under either culture condition, 3.1 μg/ml of delta-9-tetrahydrocannabinol (THC) or cannabinol (CBN) stimulated lactate secretion above control levels. Cannabidiol (CBD) stimulated lactate secretion in serum-containing but not in serum-free media. Using serum-free culture conditions, we next studied the in vitro effects of combinations of THC and either epinephrine or FSH on lactate and transferrin secretion by immature rat Sertoli cells. Co-incubation of 0.1 μM epinephrine with 0.8 or 3.1 μg/ml THC significantly stimulated lactate secretion when compared to epinephrine or THC alone, while only the high THC dose increased transferrin secretion. Moreover, co-incubation of FSH (1 μg/ml) with THC (0.8 or 3.1 μg/ml), significantly stimulated both lactate and transferrin production by immature rat Sertoli cells. These results add to the growing evidence that cannabinoids can exert direct effects on Sertoli cell function and modulate their responses to physiological stimuli.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>8231631</pmid><doi>10.1016/0024-3205(93)90585-Q</doi><tpages>9</tpages></addata></record>
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subjects Animals
Biological and medical sciences
Cannabidiol - pharmacology
Cannabinoids - pharmacology
Cannabinol - pharmacology
Cells, Cultured
Culture Media, Serum-Free
DNA - metabolism
Dronabinol - pharmacology
Drug addictions
Female
Lactates - metabolism
Male
Medical sciences
Psychotropic Drugs - pharmacology
Rats
Rats, Sprague-Dawley
Sertoli Cells - drug effects
Sertoli Cells - metabolism
Sertoli Cells - physiology
Stimulation, Chemical
Toxicology
Transferrin - metabolism
title In vitro effects of psychoactive and non-psychoactive cannabinoids on immature rat sertoli cell function
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