Cloning of a gene (PSD1) encoding phosphatidylserine decarboxylase from Saccharomyces cerevisiae by complementation of an Escherichia coli mutant

A gene (PSD1) encoding a phosphatidylserine decarboxylase of Saccharomyces cerevisiae was cloned by complementation of a conditional lethal mutation in the homologous gene in Escherichia coli strain EH150. Expression of the cDNA clone in EH150 corrected growth, phospholipid, and phosphatidylserine d...

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Veröffentlicht in:	The Journal of biological chemistry 1993-11, Vol.268 (33), p.24580-24590
Hauptverfasser:	Clancey, C J, Chang, S C, Dowhan, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Carboxy-Lyases - genetics Carboxy-Lyases - metabolism CEFALINAS CEPHALINE CLONACION CLONAGE Cloning, Molecular DNA, Complementary Enzymes and enzyme inhibitors ESCHERICHIA COLI Escherichia coli - genetics Fundamental and applied biological sciences. Psychology GENE GENES Genetic Complementation Test GENETICA GENETIQUE LIASAS LYASE Lyases Mitochondrial Proteins - genetics Mitochondrial Proteins - metabolism Molecular Sequence Data MUTACION INDUCIDA MUTANT MUTANTES Mutation MUTATION PROVOQUEE Phenotype Phospholipids - metabolism Ploidies SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism SECUENCIA NUCLEICA Sequence Homology, Amino Acid SEQUENCE NUCLEIQUE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE
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