Treatment of Human Melanocytes and S91 Melanoma Cells with the DNA Repair Enzyme T4 Endonuclease V Enhances Melanogenesis After Ultraviolet Irradiation
Tanning is a protective response of ultraviolet (UV)-irradiated skin that decreases damage from subsequent sun exposures by increasing the epidermal content of melanin, a brown-black pigment that absorbs light energy throughout the UV and visible portions of the electromagnetic spectrum. The melanin...
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| Veröffentlicht in: | Journal of investigative dermatology 1993-11, Vol.101 (5), p.666-672 |
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| creator | Gilchrest, Barbara A Zhai, Sen Eller, Mark S Yarosh, Daniel B Yaar, Mina |
| description | Tanning is a protective response of ultraviolet (UV)-irradiated skin that decreases damage from subsequent sun exposures by increasing the epidermal content of melanin, a brown-black pigment that absorbs light energy throughout the UV and visible portions of the electromagnetic spectrum. The melanin pigment is made by epidermal melanocytes and transferred to surrounding keratinocytes. The action spectrum, time course, and histologic features of tanning are well studied, but the initiating molecular events are unknown. Previous work has shown that T4 endonuclease V, a prokaryotic DNA repair enzyme that catalyzes the first and rate-limiting step in repair of UV-induced pyrimidine dimers, delivered in carrier liposomes (T4N5), enhances repair of UV-induced DNA damage in cultured human cells and protects against photocarcinogenesis in an animal model. We now report that T4N5 treatment enhances UV-induced melanogenesis, as measured by melanin content, tyrosinase activity, 14C-dopa incorporation, and visual assessment in both S91 murine melanoma cells and human melanocytes. T4NS treatment also increases cell yields following UV irradiation. These data suggest that tanning can be stimulated through enhanced DNA repair. |
| doi_str_mv | 10.1111/1523-1747.ep12371673 |
| format | Article |
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The melanin pigment is made by epidermal melanocytes and transferred to surrounding keratinocytes. The action spectrum, time course, and histologic features of tanning are well studied, but the initiating molecular events are unknown. Previous work has shown that T4 endonuclease V, a prokaryotic DNA repair enzyme that catalyzes the first and rate-limiting step in repair of UV-induced pyrimidine dimers, delivered in carrier liposomes (T4N5), enhances repair of UV-induced DNA damage in cultured human cells and protects against photocarcinogenesis in an animal model. We now report that T4N5 treatment enhances UV-induced melanogenesis, as measured by melanin content, tyrosinase activity, 14C-dopa incorporation, and visual assessment in both S91 murine melanoma cells and human melanocytes. T4NS treatment also increases cell yields following UV irradiation. These data suggest that tanning can be stimulated through enhanced DNA repair.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12371673</identifier><identifier>PMID: 8228326</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Cell Division - radiation effects ; Cells, Cultured ; Deoxyribonuclease (Pyrimidine Dimer) ; Dihydroxyphenylalanine - metabolism ; DNA Repair ; Endodeoxyribonucleases - metabolism ; endonuclease ; Fundamental and applied biological sciences. Psychology ; Humans ; irradiation ; melanin ; Melanins - analysis ; Melanins - biosynthesis ; melanocytes ; Melanocytes - metabolism ; Melanocytes - radiation effects ; Melanoma, Experimental - metabolism ; Molecular and cellular biology ; Molecular genetics ; Mutagenesis. Repair ; tanning ; Tumor Cells, Cultured ; tyrosinase ; Ultraviolet Rays ; Viral Proteins</subject><ispartof>Journal of investigative dermatology, 1993-11, Vol.101 (5), p.666-672</ispartof><rights>1993 The Society for Investigative Dermatology, Inc</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-d9b5352197fbdf5854269c008add19cd3eab818e7561146ea83514dc23e688033</citedby><cites>FETCH-LOGICAL-c386t-d9b5352197fbdf5854269c008add19cd3eab818e7561146ea83514dc23e688033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3869588$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8228326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gilchrest, Barbara A</creatorcontrib><creatorcontrib>Zhai, Sen</creatorcontrib><creatorcontrib>Eller, Mark S</creatorcontrib><creatorcontrib>Yarosh, Daniel B</creatorcontrib><creatorcontrib>Yaar, Mina</creatorcontrib><title>Treatment of Human Melanocytes and S91 Melanoma Cells with the DNA Repair Enzyme T4 Endonuclease V Enhances Melanogenesis After Ultraviolet Irradiation</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Tanning is a protective response of ultraviolet (UV)-irradiated skin that decreases damage from subsequent sun exposures by increasing the epidermal content of melanin, a brown-black pigment that absorbs light energy throughout the UV and visible portions of the electromagnetic spectrum. The melanin pigment is made by epidermal melanocytes and transferred to surrounding keratinocytes. The action spectrum, time course, and histologic features of tanning are well studied, but the initiating molecular events are unknown. Previous work has shown that T4 endonuclease V, a prokaryotic DNA repair enzyme that catalyzes the first and rate-limiting step in repair of UV-induced pyrimidine dimers, delivered in carrier liposomes (T4N5), enhances repair of UV-induced DNA damage in cultured human cells and protects against photocarcinogenesis in an animal model. We now report that T4N5 treatment enhances UV-induced melanogenesis, as measured by melanin content, tyrosinase activity, 14C-dopa incorporation, and visual assessment in both S91 murine melanoma cells and human melanocytes. T4NS treatment also increases cell yields following UV irradiation. These data suggest that tanning can be stimulated through enhanced DNA repair.</description><subject>Biological and medical sciences</subject><subject>Cell Division - radiation effects</subject><subject>Cells, Cultured</subject><subject>Deoxyribonuclease (Pyrimidine Dimer)</subject><subject>Dihydroxyphenylalanine - metabolism</subject><subject>DNA Repair</subject><subject>Endodeoxyribonucleases - metabolism</subject><subject>endonuclease</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>irradiation</subject><subject>melanin</subject><subject>Melanins - analysis</subject><subject>Melanins - biosynthesis</subject><subject>melanocytes</subject><subject>Melanocytes - metabolism</subject><subject>Melanocytes - radiation effects</subject><subject>Melanoma, Experimental - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutagenesis. Repair</subject><subject>tanning</subject><subject>Tumor Cells, Cultured</subject><subject>tyrosinase</subject><subject>Ultraviolet Rays</subject><subject>Viral Proteins</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1vEzEUtBCohMI_AMkHxG2LP9Ze7wUpCqWtVECCFHGzHPstMdr1BttbFP5I_y6Osgo3fLHfezMjvxmEXlJyQct5SwXjFW3q5gJ2lPGGyoY_QotT-zFaEMJYxQj7_hQ9S-knIVTWQp2hM8WY4kwu0MM6gskDhIzHDl9Pgwn4I_QmjHafIWETHP7a0rk3GLyCvk_4t89bnLeA339a4i-wMz7iy_BnPwBe1-XlxjDZHkwC_K2UWxNsETuK_IAAySe87DJEfNfnaO792EPGNzEa5032Y3iOnnSmT_Bivs_R3YfL9eq6uv18dbNa3laWK5kr124EF4y2TbdxnVCiZrK1hCjjHG2t42A2iipohKS0lmAUF7R2lnGQShHOz9Gbo-4ujr8mSFkPPtmyowkwTkk3khSqEAVYH4E2jilF6PQu-sHEvaZEH_LQB-P1wXj9L49CezXrT5sB3Ik0B1Dmr-e5Sdb0XSxO-XSClSVboVSBvTvCoHhx7yHqZD0UU52PYLN2o___P_4CcemnhA</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>Gilchrest, Barbara A</creator><creator>Zhai, Sen</creator><creator>Eller, Mark S</creator><creator>Yarosh, Daniel B</creator><creator>Yaar, Mina</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931101</creationdate><title>Treatment of Human Melanocytes and S91 Melanoma Cells with the DNA Repair Enzyme T4 Endonuclease V Enhances Melanogenesis After Ultraviolet Irradiation</title><author>Gilchrest, Barbara A ; Zhai, Sen ; Eller, Mark S ; Yarosh, Daniel B ; Yaar, Mina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-d9b5352197fbdf5854269c008add19cd3eab818e7561146ea83514dc23e688033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Biological and medical sciences</topic><topic>Cell Division - radiation effects</topic><topic>Cells, Cultured</topic><topic>Deoxyribonuclease (Pyrimidine Dimer)</topic><topic>Dihydroxyphenylalanine - metabolism</topic><topic>DNA Repair</topic><topic>Endodeoxyribonucleases - metabolism</topic><topic>endonuclease</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>irradiation</topic><topic>melanin</topic><topic>Melanins - analysis</topic><topic>Melanins - biosynthesis</topic><topic>melanocytes</topic><topic>Melanocytes - metabolism</topic><topic>Melanocytes - radiation effects</topic><topic>Melanoma, Experimental - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutagenesis. Repair</topic><topic>tanning</topic><topic>Tumor Cells, Cultured</topic><topic>tyrosinase</topic><topic>Ultraviolet Rays</topic><topic>Viral Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gilchrest, Barbara A</creatorcontrib><creatorcontrib>Zhai, Sen</creatorcontrib><creatorcontrib>Eller, Mark S</creatorcontrib><creatorcontrib>Yarosh, Daniel B</creatorcontrib><creatorcontrib>Yaar, Mina</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gilchrest, Barbara A</au><au>Zhai, Sen</au><au>Eller, Mark S</au><au>Yarosh, Daniel B</au><au>Yaar, Mina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Treatment of Human Melanocytes and S91 Melanoma Cells with the DNA Repair Enzyme T4 Endonuclease V Enhances Melanogenesis After Ultraviolet Irradiation</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>101</volume><issue>5</issue><spage>666</spage><epage>672</epage><pages>666-672</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>Tanning is a protective response of ultraviolet (UV)-irradiated skin that decreases damage from subsequent sun exposures by increasing the epidermal content of melanin, a brown-black pigment that absorbs light energy throughout the UV and visible portions of the electromagnetic spectrum. The melanin pigment is made by epidermal melanocytes and transferred to surrounding keratinocytes. The action spectrum, time course, and histologic features of tanning are well studied, but the initiating molecular events are unknown. Previous work has shown that T4 endonuclease V, a prokaryotic DNA repair enzyme that catalyzes the first and rate-limiting step in repair of UV-induced pyrimidine dimers, delivered in carrier liposomes (T4N5), enhances repair of UV-induced DNA damage in cultured human cells and protects against photocarcinogenesis in an animal model. We now report that T4N5 treatment enhances UV-induced melanogenesis, as measured by melanin content, tyrosinase activity, 14C-dopa incorporation, and visual assessment in both S91 murine melanoma cells and human melanocytes. T4NS treatment also increases cell yields following UV irradiation. These data suggest that tanning can be stimulated through enhanced DNA repair.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>8228326</pmid><doi>10.1111/1523-1747.ep12371673</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Cell Division - radiation effects Cells, Cultured Deoxyribonuclease (Pyrimidine Dimer) Dihydroxyphenylalanine - metabolism DNA Repair Endodeoxyribonucleases - metabolism endonuclease Fundamental and applied biological sciences. Psychology Humans irradiation melanin Melanins - analysis Melanins - biosynthesis melanocytes Melanocytes - metabolism Melanocytes - radiation effects Melanoma, Experimental - metabolism Molecular and cellular biology Molecular genetics Mutagenesis. Repair tanning Tumor Cells, Cultured tyrosinase Ultraviolet Rays Viral Proteins |
| title | Treatment of Human Melanocytes and S91 Melanoma Cells with the DNA Repair Enzyme T4 Endonuclease V Enhances Melanogenesis After Ultraviolet Irradiation |
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