Generation of primary murine CTL specific for allogeneic and xenogeneic MHC determinants upon stimulation with murine L cells transfected with class I genes
This communication describes in vitro culture conditions that permit the use of MHC gene-transfected L cells as stimulators of primary CTL responses. The use of L cells as stimulators usually results in the generation of "nonspecific" anti-L cell cytotoxicity too great to permit visualizat...
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| Veröffentlicht in: | The Journal of immunology (1950) 1985-06, Vol.134 (6), p.3557-3559 |
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| container_end_page | 3559 |
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| container_issue | 6 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 134 |
| creator | Golding, H Bluestone, J Satz, ML Singer, D Singer, A |
| description | This communication describes in vitro culture conditions that permit the use of MHC gene-transfected L cells as stimulators of primary CTL responses. The use of L cells as stimulators usually results in the generation of "nonspecific" anti-L cell cytotoxicity too great to permit visualization of antigen-specific CTL responses. This study demonstrates that the generation of such anti-L cell cytotoxicity can be abrogated by strategies that prevent the in vitro activation of self-Ia-restricted TH cells. These maneuvers allowed the use of L cell transfectants as stimulators of allospecific and xenospecific CTL precursors in primary in vitro mixed lymphocyte reaction cultures. |
| doi_str_mv | 10.4049/jimmunol.134.6.3557 |
| format | Article |
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The use of L cells as stimulators usually results in the generation of "nonspecific" anti-L cell cytotoxicity too great to permit visualization of antigen-specific CTL responses. This study demonstrates that the generation of such anti-L cell cytotoxicity can be abrogated by strategies that prevent the in vitro activation of self-Ia-restricted TH cells. 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| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1985-06, Vol.134 (6), p.3557-3559 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76055830 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Monoclonal - physiology Binding, Competitive Cytotoxicity, Immunologic Epitopes - genetics Genes, MHC Class II H-2 Antigens - genetics H-2 Antigens - immunology Histocompatibility Antigens Class II - genetics Histocompatibility Antigens Class II - immunology L Cells (Cell Line) - immunology Lymphocyte Activation Mice Mice, Inbred C57BL Swine T-Lymphocytes, Cytotoxic - classification T-Lymphocytes, Cytotoxic - immunology Transfection |
| title | Generation of primary murine CTL specific for allogeneic and xenogeneic MHC determinants upon stimulation with murine L cells transfected with class I genes |
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