Differential Enhancement of Benzophenanthridine Alkaloid Content in Cell Suspension Cultures of Sanguinaria canadensis Under Conditions of Combined Hormonal Deprivation and Fungal Elicitation

An elicitation protocol, resulting in the accumulation of sanguinarine in suspension cultures of Papaver bracteatum, was assessed for induction of the same alkaloid in Sanguinaria canadensis. Although only a trace constituent of P. bracteatum plants, sanguinarine is a major alkaloid (1-3% dry wt) of...

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Veröffentlicht in:Journal of natural products (Washington, D.C.) D.C.), 1993-08, Vol.56 (8), p.1219-1228
Hauptverfasser: Cline, Steven D, McHale, Robert J, Coscia, Carmine J
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creator Cline, Steven D
McHale, Robert J
Coscia, Carmine J
description An elicitation protocol, resulting in the accumulation of sanguinarine in suspension cultures of Papaver bracteatum, was assessed for induction of the same alkaloid in Sanguinaria canadensis. Although only a trace constituent of P. bracteatum plants, sanguinarine is a major alkaloid (1-3% dry wt) of S. canadensis rhizomes. By combining hormonal deprivation for various intervals and a 3-day fungal (Verticillium dahliae) elicitation, benzophenanthridine alkaloid accumulation was induced in S. canadensis cell suspensions. Chelirubine content increased (0.1-1.3% dry wt) consistently in elicited cell cultures while chelerythrine (0.01-0.10% dry wt) and sanguinarine (0-0.02% dry wt) levels were considerably less. Alkaloid accumulation always occurred upon removal of hormone but only at certain time intervals in the log phase upon fungal elicitation. Levels of dopamine, a precursor of the alkaloids, fluctuated over the incubation period, but displayed a 2- to 6-fold increase in cell suspensions grown without hormone. In some experiments dopamine accumulated to levels > 20% dry wt, and these increases were enhanced by the addition of fungal elicitor. Although the same fungal elicitor induces benzophenanthridines in taxonomically related S. canadensis and P. bracteatum, it did not elicit the accumulation of the same alkaloid in the two different plant cultures.
doi_str_mv 10.1021/np50098a003
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Although only a trace constituent of P. bracteatum plants, sanguinarine is a major alkaloid (1-3% dry wt) of S. canadensis rhizomes. By combining hormonal deprivation for various intervals and a 3-day fungal (Verticillium dahliae) elicitation, benzophenanthridine alkaloid accumulation was induced in S. canadensis cell suspensions. Chelirubine content increased (0.1-1.3% dry wt) consistently in elicited cell cultures while chelerythrine (0.01-0.10% dry wt) and sanguinarine (0-0.02% dry wt) levels were considerably less. Alkaloid accumulation always occurred upon removal of hormone but only at certain time intervals in the log phase upon fungal elicitation. Levels of dopamine, a precursor of the alkaloids, fluctuated over the incubation period, but displayed a 2- to 6-fold increase in cell suspensions grown without hormone. In some experiments dopamine accumulated to levels &gt; 20% dry wt, and these increases were enhanced by the addition of fungal elicitor. 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Nat. Prod</addtitle><description>An elicitation protocol, resulting in the accumulation of sanguinarine in suspension cultures of Papaver bracteatum, was assessed for induction of the same alkaloid in Sanguinaria canadensis. Although only a trace constituent of P. bracteatum plants, sanguinarine is a major alkaloid (1-3% dry wt) of S. canadensis rhizomes. By combining hormonal deprivation for various intervals and a 3-day fungal (Verticillium dahliae) elicitation, benzophenanthridine alkaloid accumulation was induced in S. canadensis cell suspensions. Chelirubine content increased (0.1-1.3% dry wt) consistently in elicited cell cultures while chelerythrine (0.01-0.10% dry wt) and sanguinarine (0-0.02% dry wt) levels were considerably less. Alkaloid accumulation always occurred upon removal of hormone but only at certain time intervals in the log phase upon fungal elicitation. Levels of dopamine, a precursor of the alkaloids, fluctuated over the incubation period, but displayed a 2- to 6-fold increase in cell suspensions grown without hormone. In some experiments dopamine accumulated to levels &gt; 20% dry wt, and these increases were enhanced by the addition of fungal elicitor. Although the same fungal elicitor induces benzophenanthridines in taxonomically related S. canadensis and P. bracteatum, it did not elicit the accumulation of the same alkaloid in the two different plant cultures.</description><subject>ALCALOIDE</subject><subject>ALCALOIDES</subject><subject>Alcaloids</subject><subject>ALKALOIDS</subject><subject>Alkaloids - chemistry</subject><subject>Alkaloids - metabolism</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Benzophenanthridines</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CELL CULTURE</subject><subject>Cells, Cultured</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>DOPAMINA</subject><subject>DOPAMINE</subject><subject>Dopamine - chemistry</subject><subject>Dopamine - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heterocyclic compounds, pigments</subject><subject>HORMONAS</subject><subject>HORMONE</subject><subject>HORMONES</subject><subject>Isoquinolines</subject><subject>METABOLITE</subject><subject>METABOLITES</subject><subject>METABOLITOS</subject><subject>Mitosporic Fungi - chemistry</subject><subject>Mitosporic Fungi - physiology</subject><subject>Other biological molecules</subject><subject>PAPAVERACEAE</subject><subject>Phenanthridines - chemistry</subject><subject>Phenanthridines - metabolism</subject><subject>Plant Growth Regulators - chemistry</subject><subject>Plant Growth Regulators - physiology</subject><subject>Plants, Medicinal - chemistry</subject><subject>Plants, Medicinal - cytology</subject><subject>Plants, Medicinal - metabolism</subject><subject>PRECURSORS</subject><subject>SECONDARY METABOLITES</subject><subject>Suspensions</subject><subject>VERTICILLIUM</subject><subject>VERTICILLIUM DAHLIAE</subject><issn>0163-3864</issn><issn>1520-6025</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU2P0zAQhiMEWsrCiRsSkg8IDigwSex8HJfulkWqAKlbiZs18Ufr3cQudoKAP8dfw9lUFQcutmbex69n9CbJ8wzeZZBn7-2BATQ1AhQPkkXGckhLyNnDZAFZWaRFXdLHyZMQbiES0LCz5KzO8wagXiR_Lo3Wyis7GOzIld2jFaqPJXGafFD2tzvslUU77L2Rxipy0d1h54wkS2eHiTOWLFXXkc0YDsoG42I9dsPoVZg8Nmh3o7HoDRKBFuXEBLK1UvnJQ5ohPrlHl65v4xeSXDvfOxvnuVQHb37gRBC0kqxGu5vG7Iwww337afJIYxfUs-N9nmxXVzfL63T95eOn5cU6RQr5kIp45rqQFa1rWqPUlCohAGKPiqqholC6LcusocCyUjFkktVVrYHSVjWtLM6T17PvwbvvowoD700QcW-0yo2BVyUwWkAWwbczKLwLwSvN4wo9-l88Az7Fxf-JK9Ivj7Zj2yt5Yo_5RP3VUccgsNM-xmPCCaN1UzUNRCydMRMG9fMko7_jZVVUjN983fDP36Ck1ZrxVeRfzLxGx3Hno-V209A8oxWN4ptZRBH4rRt9TCL8d_q_iVzEog</recordid><startdate>19930801</startdate><enddate>19930801</enddate><creator>Cline, Steven D</creator><creator>McHale, Robert J</creator><creator>Coscia, Carmine J</creator><general>American Chemical Society</general><general>American Society of Pharmacognosy</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930801</creationdate><title>Differential Enhancement of Benzophenanthridine Alkaloid Content in Cell Suspension Cultures of Sanguinaria canadensis Under Conditions of Combined Hormonal Deprivation and Fungal Elicitation</title><author>Cline, Steven D ; McHale, Robert J ; Coscia, Carmine J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a402t-c4022f3d748848adf44ecc002f34c794c3efb661940516e5a5d5878f044be9bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>ALCALOIDE</topic><topic>ALCALOIDES</topic><topic>Alcaloids</topic><topic>ALKALOIDS</topic><topic>Alkaloids - chemistry</topic><topic>Alkaloids - metabolism</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Benzophenanthridines</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CELL CULTURE</topic><topic>Cells, Cultured</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>DOPAMINA</topic><topic>DOPAMINE</topic><topic>Dopamine - chemistry</topic><topic>Dopamine - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heterocyclic compounds, pigments</topic><topic>HORMONAS</topic><topic>HORMONE</topic><topic>HORMONES</topic><topic>Isoquinolines</topic><topic>METABOLITE</topic><topic>METABOLITES</topic><topic>METABOLITOS</topic><topic>Mitosporic Fungi - chemistry</topic><topic>Mitosporic Fungi - physiology</topic><topic>Other biological molecules</topic><topic>PAPAVERACEAE</topic><topic>Phenanthridines - chemistry</topic><topic>Phenanthridines - metabolism</topic><topic>Plant Growth Regulators - chemistry</topic><topic>Plant Growth Regulators - physiology</topic><topic>Plants, Medicinal - chemistry</topic><topic>Plants, Medicinal - cytology</topic><topic>Plants, Medicinal - metabolism</topic><topic>PRECURSORS</topic><topic>SECONDARY METABOLITES</topic><topic>Suspensions</topic><topic>VERTICILLIUM</topic><topic>VERTICILLIUM DAHLIAE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cline, Steven D</creatorcontrib><creatorcontrib>McHale, Robert J</creatorcontrib><creatorcontrib>Coscia, Carmine J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of natural products (Washington, D.C.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cline, Steven D</au><au>McHale, Robert J</au><au>Coscia, Carmine J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Enhancement of Benzophenanthridine Alkaloid Content in Cell Suspension Cultures of Sanguinaria canadensis Under Conditions of Combined Hormonal Deprivation and Fungal Elicitation</atitle><jtitle>Journal of natural products (Washington, D.C.)</jtitle><addtitle>J. Nat. Prod</addtitle><date>1993-08-01</date><risdate>1993</risdate><volume>56</volume><issue>8</issue><spage>1219</spage><epage>1228</epage><pages>1219-1228</pages><issn>0163-3864</issn><eissn>1520-6025</eissn><coden>JNPRDF</coden><abstract>An elicitation protocol, resulting in the accumulation of sanguinarine in suspension cultures of Papaver bracteatum, was assessed for induction of the same alkaloid in Sanguinaria canadensis. Although only a trace constituent of P. bracteatum plants, sanguinarine is a major alkaloid (1-3% dry wt) of S. canadensis rhizomes. By combining hormonal deprivation for various intervals and a 3-day fungal (Verticillium dahliae) elicitation, benzophenanthridine alkaloid accumulation was induced in S. canadensis cell suspensions. Chelirubine content increased (0.1-1.3% dry wt) consistently in elicited cell cultures while chelerythrine (0.01-0.10% dry wt) and sanguinarine (0-0.02% dry wt) levels were considerably less. Alkaloid accumulation always occurred upon removal of hormone but only at certain time intervals in the log phase upon fungal elicitation. Levels of dopamine, a precursor of the alkaloids, fluctuated over the incubation period, but displayed a 2- to 6-fold increase in cell suspensions grown without hormone. In some experiments dopamine accumulated to levels &gt; 20% dry wt, and these increases were enhanced by the addition of fungal elicitor. Although the same fungal elicitor induces benzophenanthridines in taxonomically related S. canadensis and P. bracteatum, it did not elicit the accumulation of the same alkaloid in the two different plant cultures.</abstract><cop>Washington, DC</cop><cop>Glendale, AZ</cop><pub>American Chemical Society</pub><pmid>8229008</pmid><doi>10.1021/np50098a003</doi><tpages>10</tpages></addata></record>
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identifier ISSN: 0163-3864
ispartof Journal of natural products (Washington, D.C.), 1993-08, Vol.56 (8), p.1219-1228
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language eng
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source MEDLINE; ACS_美国化学学会期刊(与NSTL共建)
subjects ALCALOIDE
ALCALOIDES
Alcaloids
ALKALOIDS
Alkaloids - chemistry
Alkaloids - metabolism
Analytical, structural and metabolic biochemistry
Benzophenanthridines
Biological and medical sciences
Biotechnology
CELL CULTURE
Cells, Cultured
CULTIVO DE CELULAS
CULTURE DE CELLULE
DOPAMINA
DOPAMINE
Dopamine - chemistry
Dopamine - metabolism
Fundamental and applied biological sciences. Psychology
Heterocyclic compounds, pigments
HORMONAS
HORMONE
HORMONES
Isoquinolines
METABOLITE
METABOLITES
METABOLITOS
Mitosporic Fungi - chemistry
Mitosporic Fungi - physiology
Other biological molecules
PAPAVERACEAE
Phenanthridines - chemistry
Phenanthridines - metabolism
Plant Growth Regulators - chemistry
Plant Growth Regulators - physiology
Plants, Medicinal - chemistry
Plants, Medicinal - cytology
Plants, Medicinal - metabolism
PRECURSORS
SECONDARY METABOLITES
Suspensions
VERTICILLIUM
VERTICILLIUM DAHLIAE
title Differential Enhancement of Benzophenanthridine Alkaloid Content in Cell Suspension Cultures of Sanguinaria canadensis Under Conditions of Combined Hormonal Deprivation and Fungal Elicitation
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