Measurement of prolactin release and cytosolic calcium in estradiol-primed lactotrophs

We have developed a perifusion system that can measure both changes of cytosolic free calcium concentration [Ca 2+] i and prolactin release simultaneously from cultured lactotrophs. This model incorporated a commonly-used perifusion system to a spectrofluorometer. Indo-1 loaded cells were injected i...

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Veröffentlicht in:	Life sciences (1973) 1993, Vol.53 (21), p.1605-1616
Hauptverfasser:	Shin, S.H., Soukup, C., Pang, S.C., Kubiseski, T.J., Flynn, T.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Calcium - metabolism Cells, Cultured Culture Media Cytosol - metabolism Estradiol - pharmacology Extracellular Space - metabolism Fundamental and applied biological sciences. Psychology Hormones and neuropeptides. Regulation Hydrogen-Ion Concentration Hypothalamus. Hypophysis. Epiphysis. Urophysis Indoles Ionomycin - pharmacology Male Manganese - pharmacology Phenolsulfonphthalein Pituitary Gland, Anterior - metabolism Pituitary Gland, Anterior - physiology Prolactin - metabolism Rats Rats, Sprague-Dawley Signal Transduction - physiology Thyrotropin-Releasing Hormone - metabolism Vertebrates: endocrinology
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