Antibody recognition of the recombinant human nuclear antigens RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D by autoimmune sera
Five human nuclear antigens, RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D, were produced in E. coli using the expression vector pSEM. cDNAs encoding these antigens were ligated to a truncated lacZ′ gene of the vector and the ß-galactosidase fusion proteins were efficiently expressed as intracellular inclusi...
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| Veröffentlicht in: | Molecular immunology 1993, Vol.30 (16), p.1491-1498 |
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| creator | Wagatsuma, Masako Asami, Noriko Miyachi, Junko Uchida, Sanae Watanabe, Hiroshi Amann, Egon |
| description | Five human nuclear antigens, RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D, were produced in
E. coli using the expression vector pSEM. cDNAs encoding these antigens were ligated to a truncated
lacZ′ gene of the vector and the ß-galactosidase fusion proteins were efficiently expressed as intracellular inclusion bodies after isopropyl-ß-thiogalactopyranoside induction. The antibody reactivities of these fusion proteins were evaluated by Western blot and by LISA employing panel sera from patients with autoimmune diseases such as systemic lupus erythematosus, Sjögren's syndrome or mixed connective tissue disease. The three fusion proteins, RNP 70 kD, SS-B, and Sm-B, showed good reactivities in both systems, whereas the other two fusion proteins, SS-A and Sm-D, showed poor and no reactivity in both systems, respectively. It can be concluded that RNP 70 kD, SS-B and Sm-B recombinant antigens are useful reagents for the differential diagnosis of the autoimmune diseases. |
| doi_str_mv | 10.1016/0161-5890(93)90457-M |
| format | Article |
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E. coli using the expression vector pSEM. cDNAs encoding these antigens were ligated to a truncated
lacZ′ gene of the vector and the ß-galactosidase fusion proteins were efficiently expressed as intracellular inclusion bodies after isopropyl-ß-thiogalactopyranoside induction. The antibody reactivities of these fusion proteins were evaluated by Western blot and by LISA employing panel sera from patients with autoimmune diseases such as systemic lupus erythematosus, Sjögren's syndrome or mixed connective tissue disease. The three fusion proteins, RNP 70 kD, SS-B, and Sm-B, showed good reactivities in both systems, whereas the other two fusion proteins, SS-A and Sm-D, showed poor and no reactivity in both systems, respectively. It can be concluded that RNP 70 kD, SS-B and Sm-B recombinant antigens are useful reagents for the differential diagnosis of the autoimmune diseases.</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>DOI: 10.1016/0161-5890(93)90457-M</identifier><identifier>PMID: 8232335</identifier><identifier>CODEN: MOIMD5</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation ; Autoantigens - immunology ; Biological and medical sciences ; Cell Nucleus - immunology ; Escherichia coli - metabolism ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gene Expression ; Humans ; Immune Sera - immunology ; Molecular immunology ; Recombinant Proteins - immunology ; Ribonucleoproteins - immunology ; Ribonucleoproteins, Small Nuclear - immunology ; RNA, Small Cytoplasmic ; snRNP Core Proteins ; SS-B Antigen</subject><ispartof>Molecular immunology, 1993, Vol.30 (16), p.1491-1498</ispartof><rights>1993</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-b0ba69ed397de1d5b00bd77148253e6689d79487eab01d9550c5933e8262dfce3</citedby><cites>FETCH-LOGICAL-c512t-b0ba69ed397de1d5b00bd77148253e6689d79487eab01d9550c5933e8262dfce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/016158909390457M$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3831273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8232335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wagatsuma, Masako</creatorcontrib><creatorcontrib>Asami, Noriko</creatorcontrib><creatorcontrib>Miyachi, Junko</creatorcontrib><creatorcontrib>Uchida, Sanae</creatorcontrib><creatorcontrib>Watanabe, Hiroshi</creatorcontrib><creatorcontrib>Amann, Egon</creatorcontrib><title>Antibody recognition of the recombinant human nuclear antigens RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D by autoimmune sera</title><title>Molecular immunology</title><addtitle>Mol Immunol</addtitle><description>Five human nuclear antigens, RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D, were produced in
E. coli using the expression vector pSEM. cDNAs encoding these antigens were ligated to a truncated
lacZ′ gene of the vector and the ß-galactosidase fusion proteins were efficiently expressed as intracellular inclusion bodies after isopropyl-ß-thiogalactopyranoside induction. The antibody reactivities of these fusion proteins were evaluated by Western blot and by LISA employing panel sera from patients with autoimmune diseases such as systemic lupus erythematosus, Sjögren's syndrome or mixed connective tissue disease. The three fusion proteins, RNP 70 kD, SS-B, and Sm-B, showed good reactivities in both systems, whereas the other two fusion proteins, SS-A and Sm-D, showed poor and no reactivity in both systems, respectively. It can be concluded that RNP 70 kD, SS-B and Sm-B recombinant antigens are useful reagents for the differential diagnosis of the autoimmune diseases.</description><subject>Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation</subject><subject>Autoantigens - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - immunology</subject><subject>Escherichia coli - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Immune Sera - immunology</subject><subject>Molecular immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Ribonucleoproteins - immunology</subject><subject>Ribonucleoproteins, Small Nuclear - immunology</subject><subject>RNA, Small Cytoplasmic</subject><subject>snRNP Core Proteins</subject><subject>SS-B Antigen</subject><issn>0161-5890</issn><issn>1872-9142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFTEQhoNY6mn1HyjkQsRCV_OxSTY3wmmrVWhVrF6HbDLbRnezNdktnH9vzgfnsl7MTJh5Zgjvi9BLSt5RQuX7ErQSjSZvNT_RpBaqun6CFrRRrNK0Zk_RYo88Q0c5_yaESCLFITpsGGeciwV6WMYptKNf4QRuvI1hCmPEY4enO9i0hjZEGyd8Nw824ji7HmzCpRNuIWb84-t3rAj-c3GKb26q5SaflTxUZwXy68cFblfYztMYhmGOgDMk-xwddLbP8GJXj9GvTx9_nn-urr5dfjlfXlVOUDZVLWmt1OC5Vh6oFy0hrVeK1g0THKRstFe6bhTYllCvhSBOaM6hYZL5zgE_Rm-2d-_T-HeGPJkhZAd9byOMczZKEkEE1_8FqVSaSdIUsN6CLo05J-jMfQqDTStDiVn7Ytaim7XoRnOz8cVcl7VXu_tzO4DfL-2MKPPXu7nNzvZdstGFvMd4wylTvGAfthgU0R4CJJNdgOjAh2LWZPwYHv_HPzsDpnY</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Wagatsuma, Masako</creator><creator>Asami, Noriko</creator><creator>Miyachi, Junko</creator><creator>Uchida, Sanae</creator><creator>Watanabe, Hiroshi</creator><creator>Amann, Egon</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Antibody recognition of the recombinant human nuclear antigens RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D by autoimmune sera</title><author>Wagatsuma, Masako ; Asami, Noriko ; Miyachi, Junko ; Uchida, Sanae ; Watanabe, Hiroshi ; Amann, Egon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-b0ba69ed397de1d5b00bd77148253e6689d79487eab01d9550c5933e8262dfce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation</topic><topic>Autoantigens - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleus - immunology</topic><topic>Escherichia coli - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Immune Sera - immunology</topic><topic>Molecular immunology</topic><topic>Recombinant Proteins - immunology</topic><topic>Ribonucleoproteins - immunology</topic><topic>Ribonucleoproteins, Small Nuclear - immunology</topic><topic>RNA, Small Cytoplasmic</topic><topic>snRNP Core Proteins</topic><topic>SS-B Antigen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wagatsuma, Masako</creatorcontrib><creatorcontrib>Asami, Noriko</creatorcontrib><creatorcontrib>Miyachi, Junko</creatorcontrib><creatorcontrib>Uchida, Sanae</creatorcontrib><creatorcontrib>Watanabe, Hiroshi</creatorcontrib><creatorcontrib>Amann, Egon</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wagatsuma, Masako</au><au>Asami, Noriko</au><au>Miyachi, Junko</au><au>Uchida, Sanae</au><au>Watanabe, Hiroshi</au><au>Amann, Egon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibody recognition of the recombinant human nuclear antigens RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D by autoimmune sera</atitle><jtitle>Molecular immunology</jtitle><addtitle>Mol Immunol</addtitle><date>1993</date><risdate>1993</risdate><volume>30</volume><issue>16</issue><spage>1491</spage><epage>1498</epage><pages>1491-1498</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><coden>MOIMD5</coden><abstract>Five human nuclear antigens, RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D, were produced in
E. coli using the expression vector pSEM. cDNAs encoding these antigens were ligated to a truncated
lacZ′ gene of the vector and the ß-galactosidase fusion proteins were efficiently expressed as intracellular inclusion bodies after isopropyl-ß-thiogalactopyranoside induction. The antibody reactivities of these fusion proteins were evaluated by Western blot and by LISA employing panel sera from patients with autoimmune diseases such as systemic lupus erythematosus, Sjögren's syndrome or mixed connective tissue disease. The three fusion proteins, RNP 70 kD, SS-B, and Sm-B, showed good reactivities in both systems, whereas the other two fusion proteins, SS-A and Sm-D, showed poor and no reactivity in both systems, respectively. It can be concluded that RNP 70 kD, SS-B and Sm-B recombinant antigens are useful reagents for the differential diagnosis of the autoimmune diseases.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8232335</pmid><doi>10.1016/0161-5890(93)90457-M</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular immunology, 1993, Vol.30 (16), p.1491-1498 |
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| language | eng |
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| subjects | Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation Autoantigens - immunology Biological and medical sciences Cell Nucleus - immunology Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Expression Humans Immune Sera - immunology Molecular immunology Recombinant Proteins - immunology Ribonucleoproteins - immunology Ribonucleoproteins, Small Nuclear - immunology RNA, Small Cytoplasmic snRNP Core Proteins SS-B Antigen |
| title | Antibody recognition of the recombinant human nuclear antigens RNP 70 kD, SS-A, SS-B, Sm-B and Sm-D by autoimmune sera |
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