Developmental Progression of DNA Puffs in Sciara coprophila: Amplification and Transcription
DNA amplification for two major DNA puffs (II/2B and II/9A) of the fungus fly Sciara coprophila increases steeply from 17 to 19 days after hatching (18°C), resulting in almost 20-fold more DNA at these loci in mature larval salivary glands than in adult tissues. At 19 days after hatching when gene a...
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| Veröffentlicht in: | Developmental biology 1993-11, Vol.160 (1), p.73-84 |
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| creator | Wu, Nan Liang, Chun Dibartolomeis, Susan M. Smith, Heidi S. Gerbi, Susan A. |
| description | DNA amplification for two major DNA puffs (II/2B and II/9A) of the fungus fly Sciara coprophila increases steeply from 17 to 19 days after hatching (18°C), resulting in almost 20-fold more DNA at these loci in mature larval salivary glands than in adult tissues. At 19 days after hatching when gene amplification reaches a plateau, there is a burst in the amount of mRNA encoded by these two DNA puffs. Expansion of the two puffs coincides with the increase in transcription rather than reinitiation of DNA replication. In contrast, an RNA puff (III/9B) undergoes no DNA amplification, and the burst in RNA produced by this locus occurs slightly later, at 20 days after hatching. The progression of cytological puffing and associated molecular events of DNA amplification and transcription correlate with features of the external phenotype of the larvae, especially the number of pigment granules in the eyespots that are the anlage to the adult eyes. The sequential and coordinated regulation of DNA puff replication and transcription is discussed. |
| doi_str_mv | 10.1006/dbio.1993.1287 |
| format | Article |
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At 19 days after hatching when gene amplification reaches a plateau, there is a burst in the amount of mRNA encoded by these two DNA puffs. Expansion of the two puffs coincides with the increase in transcription rather than reinitiation of DNA replication. In contrast, an RNA puff (III/9B) undergoes no DNA amplification, and the burst in RNA produced by this locus occurs slightly later, at 20 days after hatching. The progression of cytological puffing and associated molecular events of DNA amplification and transcription correlate with features of the external phenotype of the larvae, especially the number of pigment granules in the eyespots that are the anlage to the adult eyes. The sequential and coordinated regulation of DNA puff replication and transcription is discussed.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1006/dbio.1993.1287</identifier><identifier>PMID: 8224550</identifier><identifier>CODEN: DEBIAO</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; Cloning, Molecular ; Diptera ; Diptera - embryology ; Diptera - genetics ; DNA - physiology ; DNA Replication ; Eye - embryology ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; Insecta ; Invertebrates ; Larva ; Phenotype ; Physiology. Development ; Salivary Glands - embryology ; Salivary Glands - metabolism ; Sciaridae ; Transcription, Genetic</subject><ispartof>Developmental biology, 1993-11, Vol.160 (1), p.73-84</ispartof><rights>1993 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-5a84bad0ee442a1ede4e66b6974eaeaa109ca8854451ff01f3ff349d715ef62b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/dbio.1993.1287$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3834960$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8224550$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Nan</creatorcontrib><creatorcontrib>Liang, Chun</creatorcontrib><creatorcontrib>Dibartolomeis, Susan M.</creatorcontrib><creatorcontrib>Smith, Heidi S.</creatorcontrib><creatorcontrib>Gerbi, Susan A.</creatorcontrib><title>Developmental Progression of DNA Puffs in Sciara coprophila: Amplification and Transcription</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>DNA amplification for two major DNA puffs (II/2B and II/9A) of the fungus fly Sciara coprophila increases steeply from 17 to 19 days after hatching (18°C), resulting in almost 20-fold more DNA at these loci in mature larval salivary glands than in adult tissues. At 19 days after hatching when gene amplification reaches a plateau, there is a burst in the amount of mRNA encoded by these two DNA puffs. Expansion of the two puffs coincides with the increase in transcription rather than reinitiation of DNA replication. In contrast, an RNA puff (III/9B) undergoes no DNA amplification, and the burst in RNA produced by this locus occurs slightly later, at 20 days after hatching. The progression of cytological puffing and associated molecular events of DNA amplification and transcription correlate with features of the external phenotype of the larvae, especially the number of pigment granules in the eyespots that are the anlage to the adult eyes. The sequential and coordinated regulation of DNA puff replication and transcription is discussed.</description><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Diptera</subject><subject>Diptera - embryology</subject><subject>Diptera - genetics</subject><subject>DNA - physiology</subject><subject>DNA Replication</subject><subject>Eye - embryology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>Larva</subject><subject>Phenotype</subject><subject>Physiology. Development</subject><subject>Salivary Glands - embryology</subject><subject>Salivary Glands - metabolism</subject><subject>Sciaridae</subject><subject>Transcription, Genetic</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo6-zq1ZuQg3jrsdL5mLS3YddVYdEFV_AghOp0RSPdnTbpWfDf280MexNPBVVPFfU-jL0QsBUA5k3XxrQVTSO3ora7R2wjoNGVNurbY7YBEHUlDJin7LyUXwAgrZVn7MzWtdIaNuz7Fd1Tn6aBxhl7fpvTj0ylxDTyFPjVpz2_PYRQeBz5Fx8xI_dpymn6GXt8y_fD1McQPc7rAo4dv8s4Fp_jtHaesScB-0LPT_WCfb1-d3f5obr5_P7j5f6m8rJp5kqjVS12QKRUjYI6UmRMa5qdIiTEJZFHa7VSWoQAIsgQpGq6ndAUTN3KC_b6eHf57PeByuyGWDz1PY6UDsXtDChtavtfUBgLEkSzgNsj6HMqJVNwU44D5j9OgFu9u9W7W7271fuy8PJ0-dAO1D3gJ9HL_NVpjsVjHxZNPpYHTNolkFkxe8Ro0XUfKbviI42eupjJz65L8V8f_AXZaZ9P</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>Wu, Nan</creator><creator>Liang, Chun</creator><creator>Dibartolomeis, Susan M.</creator><creator>Smith, Heidi S.</creator><creator>Gerbi, Susan A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19931101</creationdate><title>Developmental Progression of DNA Puffs in Sciara coprophila: Amplification and Transcription</title><author>Wu, Nan ; Liang, Chun ; Dibartolomeis, Susan M. ; Smith, Heidi S. ; Gerbi, Susan A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-5a84bad0ee442a1ede4e66b6974eaeaa109ca8854451ff01f3ff349d715ef62b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Diptera</topic><topic>Diptera - embryology</topic><topic>Diptera - genetics</topic><topic>DNA - physiology</topic><topic>DNA Replication</topic><topic>Eye - embryology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Amplification</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>Larva</topic><topic>Phenotype</topic><topic>Physiology. Development</topic><topic>Salivary Glands - embryology</topic><topic>Salivary Glands - metabolism</topic><topic>Sciaridae</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Nan</creatorcontrib><creatorcontrib>Liang, Chun</creatorcontrib><creatorcontrib>Dibartolomeis, Susan M.</creatorcontrib><creatorcontrib>Smith, Heidi S.</creatorcontrib><creatorcontrib>Gerbi, Susan A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Nan</au><au>Liang, Chun</au><au>Dibartolomeis, Susan M.</au><au>Smith, Heidi S.</au><au>Gerbi, Susan A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental Progression of DNA Puffs in Sciara coprophila: Amplification and Transcription</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>160</volume><issue>1</issue><spage>73</spage><epage>84</epage><pages>73-84</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><coden>DEBIAO</coden><abstract>DNA amplification for two major DNA puffs (II/2B and II/9A) of the fungus fly Sciara coprophila increases steeply from 17 to 19 days after hatching (18°C), resulting in almost 20-fold more DNA at these loci in mature larval salivary glands than in adult tissues. At 19 days after hatching when gene amplification reaches a plateau, there is a burst in the amount of mRNA encoded by these two DNA puffs. Expansion of the two puffs coincides with the increase in transcription rather than reinitiation of DNA replication. In contrast, an RNA puff (III/9B) undergoes no DNA amplification, and the burst in RNA produced by this locus occurs slightly later, at 20 days after hatching. The progression of cytological puffing and associated molecular events of DNA amplification and transcription correlate with features of the external phenotype of the larvae, especially the number of pigment granules in the eyespots that are the anlage to the adult eyes. The sequential and coordinated regulation of DNA puff replication and transcription is discussed.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>8224550</pmid><doi>10.1006/dbio.1993.1287</doi><tpages>12</tpages></addata></record> |
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| ispartof | Developmental biology, 1993-11, Vol.160 (1), p.73-84 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biochemistry. Physiology. Immunology Biological and medical sciences Cloning, Molecular Diptera Diptera - embryology Diptera - genetics DNA - physiology DNA Replication Eye - embryology Fundamental and applied biological sciences. Psychology Gene Amplification Insecta Invertebrates Larva Phenotype Physiology. Development Salivary Glands - embryology Salivary Glands - metabolism Sciaridae Transcription, Genetic |
| title | Developmental Progression of DNA Puffs in Sciara coprophila: Amplification and Transcription |
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