Determination of the structure of the carbohydrate chains of prostaglandin endoperoxide synthase from sheep
Prostaglandin endoperoxide synthase was isolated from sheep seminal vesicles. Sugar analysis of the glycoprotein revealed the presence of mannose and N‐acetylglucosamine only. The carbohydrate moiety was released from the polypeptide backbone by hydrazinolysis. After re‐N‐acetylation and reduction,...
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| Veröffentlicht in: | European journal of biochemistry 1985-01, Vol.147 (3), p.569-574 |
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| container_title | European journal of biochemistry |
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| creator | Mutsaers, J.H.G.M Halbeek, H. van Kamerling, J.P Vliegenthart, J.F.G |
| description | Prostaglandin endoperoxide synthase was isolated from sheep seminal vesicles. Sugar analysis of the glycoprotein revealed the presence of mannose and N‐acetylglucosamine only. The carbohydrate moiety was released from the polypeptide backbone by hydrazinolysis. After re‐N‐acetylation and reduction, the resulting mixture of oligosaccharide‐alditols was fractionated on Bio‐Gel P‐4 and their structures were investigated by 500‐MHz 1H‐NMR spectroscopy. The carbohydrate chains turned out to be of the oligomannoside type containing six to nine mannose residues. The largest and most abundant compound was established to be:
For the smaller structures heterogeneity occurs with respect to the outer α(1 → 2)‐linked mannose residues. Furthermore, a small amount of Man6GlcNAc‐ol (artefact of the hydrazinolysis procedure) was detected by 1H‐NMR spectroscopy and fast atom bombardment mass spectrometry. |
| doi_str_mv | 10.1111/j.0014-2956.1985.00569.x |
| format | Article |
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For the smaller structures heterogeneity occurs with respect to the outer α(1 → 2)‐linked mannose residues. Furthermore, a small amount of Man6GlcNAc‐ol (artefact of the hydrazinolysis procedure) was detected by 1H‐NMR spectroscopy and fast atom bombardment mass spectrometry.</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1111/j.0014-2956.1985.00569.x</identifier><identifier>PMID: 3920047</identifier><identifier>CODEN: EJBCAI</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; carbohydrates ; Carbohydrates - analysis ; Chemical Phenomena ; Chemistry ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Magnetic Resonance Spectroscopy ; Male ; manmade structures ; Mass Spectrometry ; Monosaccharides - analysis ; Oligosaccharides - analysis ; Oxidoreductases ; prostaglandin synthase ; Prostaglandin-Endoperoxide Synthases ; Sheep</subject><ispartof>European journal of biochemistry, 1985-01, Vol.147 (3), p.569-574</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4679-3eea071a41b25891236faa4e9efa64fe87c3d5b9195d18384ade7afc969007d3</citedby><cites>FETCH-LOGICAL-c4679-3eea071a41b25891236faa4e9efa64fe87c3d5b9195d18384ade7afc969007d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8433620$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3920047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mutsaers, J.H.G.M</creatorcontrib><creatorcontrib>Halbeek, H. van</creatorcontrib><creatorcontrib>Kamerling, J.P</creatorcontrib><creatorcontrib>Vliegenthart, J.F.G</creatorcontrib><title>Determination of the structure of the carbohydrate chains of prostaglandin endoperoxide synthase from sheep</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>Prostaglandin endoperoxide synthase was isolated from sheep seminal vesicles. Sugar analysis of the glycoprotein revealed the presence of mannose and N‐acetylglucosamine only. The carbohydrate moiety was released from the polypeptide backbone by hydrazinolysis. After re‐N‐acetylation and reduction, the resulting mixture of oligosaccharide‐alditols was fractionated on Bio‐Gel P‐4 and their structures were investigated by 500‐MHz 1H‐NMR spectroscopy. The carbohydrate chains turned out to be of the oligomannoside type containing six to nine mannose residues. The largest and most abundant compound was established to be:
For the smaller structures heterogeneity occurs with respect to the outer α(1 → 2)‐linked mannose residues. Furthermore, a small amount of Man6GlcNAc‐ol (artefact of the hydrazinolysis procedure) was detected by 1H‐NMR spectroscopy and fast atom bombardment mass spectrometry.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>carbohydrates</subject><subject>Carbohydrates - analysis</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Male</subject><subject>manmade structures</subject><subject>Mass Spectrometry</subject><subject>Monosaccharides - analysis</subject><subject>Oligosaccharides - analysis</subject><subject>Oxidoreductases</subject><subject>prostaglandin synthase</subject><subject>Prostaglandin-Endoperoxide Synthases</subject><subject>Sheep</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUE1v1DAQtRCoLIWfgMgBccvirzjxgQOUtiBV4tBytmbtceMlH4udiN1_j9Nd9owv9vi9N2_mEVIwumb5fNyuKWWy5LpSa6abKpeV0uv9M7JiUvCSUSGek9WZ9JK8SmlLKVVa1RfkQmhOqaxX5NdXnDD2YYApjEMx-mJqsUhTnO00R_z3YSFuxvbgIky5aCEMaYF2cUwTPHYwuDAUOLhxh3HcB5dbHIaphYSFj2NfpBZx95q88NAlfHO6L8nDzfXD1bfy7sft96vPd6WVqtalQARaM5Bsw6tGMy6UB5Co0YOSHpvaCldtNNOVY41oJDiswVutNKW1E5fkw7Ftnu73jGkyfUgWuzwljnMytaKy4pJnYnMk2rxGiujNLoYe4sEwapaYzdYsCZolQbPEbJ5iNvssfXvymDc9urPwlGvG359wSBY6H2GwIZ1pjRRCcZppn460P6HDw3_bm5vrL_f5lfXvjnoPo4HHmC1-3nPKBOUq76io-AsLV6Ow</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Mutsaers, J.H.G.M</creator><creator>Halbeek, H. van</creator><creator>Kamerling, J.P</creator><creator>Vliegenthart, J.F.G</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850101</creationdate><title>Determination of the structure of the carbohydrate chains of prostaglandin endoperoxide synthase from sheep</title><author>Mutsaers, J.H.G.M ; Halbeek, H. van ; Kamerling, J.P ; Vliegenthart, J.F.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4679-3eea071a41b25891236faa4e9efa64fe87c3d5b9195d18384ade7afc969007d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>carbohydrates</topic><topic>Carbohydrates - analysis</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Male</topic><topic>manmade structures</topic><topic>Mass Spectrometry</topic><topic>Monosaccharides - analysis</topic><topic>Oligosaccharides - analysis</topic><topic>Oxidoreductases</topic><topic>prostaglandin synthase</topic><topic>Prostaglandin-Endoperoxide Synthases</topic><topic>Sheep</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mutsaers, J.H.G.M</creatorcontrib><creatorcontrib>Halbeek, H. van</creatorcontrib><creatorcontrib>Kamerling, J.P</creatorcontrib><creatorcontrib>Vliegenthart, J.F.G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mutsaers, J.H.G.M</au><au>Halbeek, H. van</au><au>Kamerling, J.P</au><au>Vliegenthart, J.F.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of the structure of the carbohydrate chains of prostaglandin endoperoxide synthase from sheep</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>147</volume><issue>3</issue><spage>569</spage><epage>574</epage><pages>569-574</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><coden>EJBCAI</coden><abstract>Prostaglandin endoperoxide synthase was isolated from sheep seminal vesicles. Sugar analysis of the glycoprotein revealed the presence of mannose and N‐acetylglucosamine only. The carbohydrate moiety was released from the polypeptide backbone by hydrazinolysis. After re‐N‐acetylation and reduction, the resulting mixture of oligosaccharide‐alditols was fractionated on Bio‐Gel P‐4 and their structures were investigated by 500‐MHz 1H‐NMR spectroscopy. The carbohydrate chains turned out to be of the oligomannoside type containing six to nine mannose residues. The largest and most abundant compound was established to be:
For the smaller structures heterogeneity occurs with respect to the outer α(1 → 2)‐linked mannose residues. Furthermore, a small amount of Man6GlcNAc‐ol (artefact of the hydrazinolysis procedure) was detected by 1H‐NMR spectroscopy and fast atom bombardment mass spectrometry.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>3920047</pmid><doi>10.1111/j.0014-2956.1985.00569.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European journal of biochemistry, 1985-01, Vol.147 (3), p.569-574 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences carbohydrates Carbohydrates - analysis Chemical Phenomena Chemistry Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Magnetic Resonance Spectroscopy Male manmade structures Mass Spectrometry Monosaccharides - analysis Oligosaccharides - analysis Oxidoreductases prostaglandin synthase Prostaglandin-Endoperoxide Synthases Sheep |
| title | Determination of the structure of the carbohydrate chains of prostaglandin endoperoxide synthase from sheep |
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