Simultaneous Detection of High-Resolution R-Banding and Fluorescence in Situ Hybridization Signals After Fluorouracil-Induced Cellular Synchronization

A method for simultaneous detection of fluorescence in situ hybridization of DNA probes and high resolution fluorescent R banding is described. Human lymphocytes were stimulated with phytohemagglutinin and synchronized using a fluorouracil block followed by exposure to bromodeoxyuridine and Hoechst...

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Veröffentlicht in:	Hereditas 1993-01, Vol.119 (1), p.89-94
Hauptverfasser:	Larramendy, Marcelo L., Nylund, Stella J., Armstrong, Elina, Knuutila, Sakari
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell Division - drug effects Cells, Cultured Chromosome Banding - methods Cytogenetics DNA Probes Fluorouracil Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Human Humans In Situ Hybridization, Fluorescence - methods Lymphocytes - cytology Lymphocytes - drug effects Metaphase
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