A rapid and sensitive determination of salmon calcitonin in solutions containing bovine serum albumin or gelatin
A sensitive and selective reversed phase high performance liquid chromatographic method was introduced for the determination of salmon calcitonin (sCT) in solutions containing bovine serum albumin or gelatin. The method was based on the sensitive fluorescence detection of sCT by postcolumn derivatiz...
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| Veröffentlicht in: | Biomedical chromatography 1993-07, Vol.7 (4), p.229-230 |
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| container_title | Biomedical chromatography |
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| creator | Fukuda, Terumi Yun, Arifuku Imai, Kazuhiro |
| description | A sensitive and selective reversed phase high performance liquid chromatographic method was introduced for the determination of salmon calcitonin (sCT) in solutions containing bovine serum albumin or gelatin. The method was based on the sensitive fluorescence detection of sCT by postcolumn derivatization with o‐phthal(di)aldehyde. The sample was loaded onto a reversed phase column, purified by column switching and separated with linear gradient elution using the ion pair technique. A linear relationship was obtained between the peak height and the amount injected in the concentration range 1–40 ng. The detection limit was 10 ng/mL (S/N = 3) at a sample injection volume of 100 μL. |
| doi_str_mv | 10.1002/bmc.1130070412 |
| format | Article |
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The method was based on the sensitive fluorescence detection of sCT by postcolumn derivatization with o‐phthal(di)aldehyde. The sample was loaded onto a reversed phase column, purified by column switching and separated with linear gradient elution using the ion pair technique. A linear relationship was obtained between the peak height and the amount injected in the concentration range 1–40 ng. The detection limit was 10 ng/mL (S/N = 3) at a sample injection volume of 100 μL.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.1130070412</identifier><identifier>PMID: 8219703</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Calcitonin - analysis ; Cattle ; Chromatography, High Pressure Liquid ; Gelatin - analysis ; o-Phthalaldehyde ; Serum Albumin, Bovine - analysis ; Spectrometry, Fluorescence</subject><ispartof>Biomedical chromatography, 1993-07, Vol.7 (4), p.229-230</ispartof><rights>Copyright © 1993 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3782-4a7dacde839eb609946aaf303ce38c1c49b604b3249e4a33b812060005ca95653</citedby><cites>FETCH-LOGICAL-c3782-4a7dacde839eb609946aaf303ce38c1c49b604b3249e4a33b812060005ca95653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.1130070412$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.1130070412$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8219703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukuda, Terumi</creatorcontrib><creatorcontrib>Yun, Arifuku</creatorcontrib><creatorcontrib>Imai, Kazuhiro</creatorcontrib><title>A rapid and sensitive determination of salmon calcitonin in solutions containing bovine serum albumin or gelatin</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>A sensitive and selective reversed phase high performance liquid chromatographic method was introduced for the determination of salmon calcitonin (sCT) in solutions containing bovine serum albumin or gelatin. The method was based on the sensitive fluorescence detection of sCT by postcolumn derivatization with o‐phthal(di)aldehyde. The sample was loaded onto a reversed phase column, purified by column switching and separated with linear gradient elution using the ion pair technique. A linear relationship was obtained between the peak height and the amount injected in the concentration range 1–40 ng. The detection limit was 10 ng/mL (S/N = 3) at a sample injection volume of 100 μL.</description><subject>Animals</subject><subject>Calcitonin - analysis</subject><subject>Cattle</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Gelatin - analysis</subject><subject>o-Phthalaldehyde</subject><subject>Serum Albumin, Bovine - analysis</subject><subject>Spectrometry, Fluorescence</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1P3DAQxS3Uim5pr9wq-dRbtv7IxvGRroBWXUCIVhytiTOLDI69tRMo_z1GuwJxqjTSjGbe-0nzCDnkbM4ZE9-6wc45l4wpVnOxR2acaV2xlvF3ZMZEoyvZKv2BfMz5ljGmG6H2yX4ruFZMzsjmiCbYuJ5C6GnGkN3o7pH2OGIaXIDRxUDjmmbwQ5kseOvGGFygpXL007MgUxvDCK6sb2gX713AwkrTQMF3U8HQmOgN-kILn8j7NfiMn3f9gPw5Of69_FGtLk5_Lo9WlZWqFVUNqgfbYys1dk15qW4A1pJJi7K13Na6bOtOilpjDVJ2LResKQ8uLOhFs5AH5OuWu0nx74R5NIPLFr2HgHHKRhW7UKItwvlWaFPMOeHabJIbID0azsxzxKZEbF4jLoYvO_LUDdi_yHeZlrve3h-cx8f_0Mz3s-UbdrX1ujzivxcvpDvTKKkW5vr81Jxd_7q65M3KnMsnpGeYKA</recordid><startdate>199307</startdate><enddate>199307</enddate><creator>Fukuda, Terumi</creator><creator>Yun, Arifuku</creator><creator>Imai, Kazuhiro</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199307</creationdate><title>A rapid and sensitive determination of salmon calcitonin in solutions containing bovine serum albumin or gelatin</title><author>Fukuda, Terumi ; Yun, Arifuku ; Imai, Kazuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3782-4a7dacde839eb609946aaf303ce38c1c49b604b3249e4a33b812060005ca95653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Calcitonin - analysis</topic><topic>Cattle</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Gelatin - analysis</topic><topic>o-Phthalaldehyde</topic><topic>Serum Albumin, Bovine - analysis</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fukuda, Terumi</creatorcontrib><creatorcontrib>Yun, Arifuku</creatorcontrib><creatorcontrib>Imai, Kazuhiro</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukuda, Terumi</au><au>Yun, Arifuku</au><au>Imai, Kazuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid and sensitive determination of salmon calcitonin in solutions containing bovine serum albumin or gelatin</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>1993-07</date><risdate>1993</risdate><volume>7</volume><issue>4</issue><spage>229</spage><epage>230</epage><pages>229-230</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>A sensitive and selective reversed phase high performance liquid chromatographic method was introduced for the determination of salmon calcitonin (sCT) in solutions containing bovine serum albumin or gelatin. The method was based on the sensitive fluorescence detection of sCT by postcolumn derivatization with o‐phthal(di)aldehyde. The sample was loaded onto a reversed phase column, purified by column switching and separated with linear gradient elution using the ion pair technique. A linear relationship was obtained between the peak height and the amount injected in the concentration range 1–40 ng. 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| fulltext | fulltext |
| identifier | ISSN: 0269-3879 |
| ispartof | Biomedical chromatography, 1993-07, Vol.7 (4), p.229-230 |
| issn | 0269-3879 1099-0801 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76042728 |
| source | Wiley-Blackwell Journals; MEDLINE |
| subjects | Animals Calcitonin - analysis Cattle Chromatography, High Pressure Liquid Gelatin - analysis o-Phthalaldehyde Serum Albumin, Bovine - analysis Spectrometry, Fluorescence |
| title | A rapid and sensitive determination of salmon calcitonin in solutions containing bovine serum albumin or gelatin |
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