Expression of a mouse-channel catfish chimeric IgM molecule in a mouse myeloma cell

Fusion genes encoding a murine V H domain and the constant region domains of the μ chain from the channel catfish, Ictalurus punctatus, were stably expressed in the λ light chain producing mouse myeloma cell line J558L. Although the pathways of pre-mRNA processing for expression of membrane (μm) and...

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Veröffentlicht in:	Molecular immunology 1993, Vol.30 (16), p.1405-1417
Hauptverfasser:	Ledford, Barry E., Magor, Brad G., Middleton, Darlene L., Miller, Ronald L., Wilson, Melanie R., Miller, Norman W., Clem, L.William, Warr, Gregory W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, immunoglobulins Base Sequence Biological and medical sciences Chimera Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Expression Ictaluridae Ictalurus punctatus Immunoglobulin lambda-Chains Immunoglobulin M - genetics Immunoglobulin M - metabolism Immunoglobulin mu-Chains Mice Molecular immunology Molecular Sequence Data Plasmacytoma - genetics Plasmacytoma - immunology Plasmacytoma - metabolism RNA, Messenger - analysis Sequence Alignment Staphylococcal Protein A - metabolism Structure Sulfur Radioisotopes Transfection Tumor Cells, Cultured
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