Cloning and functional expression of a Dictyostelium discoideum protein tyrosine phosphatase

Using polymerase chain reaction methods, we cloned a 1.7-kilobase cDNA, denoted DdPTPa, that has high homology with other known eukaryotic protein tyrosine phosphatases. DdPTPa possess a 241-amino acid protein tyrosine phosphatase domain located in the C terminus, which exhibits a 39-43% amino acid...

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Veröffentlicht in:	The Journal of biological chemistry 1993-10, Vol.268 (30), p.22680-22685
Hauptverfasser:	Ramalingam, R, Shaw, D.R., Ennis, H.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals ARN MENSAJERO ARN MESSAGER Base Sequence Biological and medical sciences CLONACION CLONAGE Cloning, Molecular - methods Dictyostelium - enzymology Dictyostelium - genetics Dictyostelium - growth & development Dictyostelium discoideum DNA Primers Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors ESCHERICHIA Escherichia coli ESTERASAS ESTERASE EXPRESION GENICA EXPRESSION DES GENES FOSFORILACION Fundamental and applied biological sciences. Psychology GENE Gene Expression GENES Hydrolases Kinetics MIXOMICETES Molecular Sequence Data Molecular Weight MYXOMYCETES PHOSPHORYLATION Polymerase Chain Reaction Protein Tyrosine Phosphatases - biosynthesis Protein Tyrosine Phosphatases - isolation & purification Protein Tyrosine Phosphatases - metabolism REACCIONES QUIMICAS REACTION CHIMIQUE Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism RNA, Messenger - biosynthesis RNA, Messenger - metabolism SECUENCIA NUCLEICA Sequence Homology, Amino Acid SEQUENCE NUCLEIQUE TIROSINA TRANSFERENCIA DE GENES TRANSFERT DE GENE TYROSINE Vanadates - pharmacology
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