Pharmacological and chemical screening of Byrsonima crassifolia, a medicinal tree from Mexico. Part I
Leaf and bark extracts of Byrsonima crassifolia displayed concentration-dependent, spasmogenic effects on rat fundus in vitro and biphasic effects on rat jejunum and ileum in vitro. Dose-related in vivo effects in intact rats using hippocratic screening were: decrease in motor activity, mild analges...
Gespeichert in:
| Veröffentlicht in: | Journal of ethnopharmacology 1993-06, Vol.39 (2), p.141-158 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 158 |
|---|---|
| container_issue | 2 |
| container_start_page | 141 |
| container_title | Journal of ethnopharmacology |
| container_volume | 39 |
| creator | Béjar, Ezra Malone, Marvin H. |
| description | Leaf and bark extracts of
Byrsonima crassifolia displayed concentration-dependent, spasmogenic effects on rat fundus in vitro and biphasic effects on rat jejunum and ileum in vitro. Dose-related in vivo effects in intact rats using hippocratic screening were: decrease in motor activity, mild analgesia, back tonus, enophthalmos, reversable palpebral ptosis, ear blanching, Robichaud positive, catalepsy (awake) and strong hypothermia. Rat fundus in vitro was used as the bioassay to carry out an activity-directed separation. Bioactive material was concentrated in a 2% acetic acid leaf extract (HOAcE). Potency of HOAcE was increased by the presence of pargyline in the bathing solution. HOAcE was antagonized noncompetively by l(I-naphthyl) piperazine (I-NP) and cyproheptadine and antagonized competitively by atropine (ATR). Cumulative concentration-response curves of HOAcE and serotonin (5-HT) did not show significant departure from parallelism (
P > 0.1) and 5-HT potency was 6040 times that of HOAcE (95% confidence limits: 4620–7850). Solvent extraction of HOAcE split the spasmogenic activity of HOAcE into two types: (i) high-efficacy, low-potency,
n-butanol-extracted, pargyline- and 1-NP-sensitive, ATR-insensitive activity, and (ii) low-efficacy, high-potency, ethyl acetate-extracted, pargyline-insensitive, ATR- and 1-NP-sensitive activity. HOAcE lacked muscarinic and nicotinic effects on rat jejunum and frog rectus abdominis. Results suggest the presence of more than one spasmogenic compound in the plant. |
| doi_str_mv | 10.1016/0378-8741(93)90029-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76035248</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0378874193900295</els_id><sourcerecordid>76035248</sourcerecordid><originalsourceid>FETCH-LOGICAL-c272t-6386d73bf72301ce0d9861f46eff30b6cd1acec1d029c0c6a0a88927d21f1f343</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMoun78A4WcRMGu-eg26UVQ8QtW9KDnkJ1ONNI2mnRF_71Zd_HoaRjmmRneh5B9zsac8eqUSaULrUp-VMvjmjFRF5M1MuJaiUJNlFwnoz9ki2yn9MYYU7xkm2RTl1yIUo0IPr7a2FkIbXjxYFtq-4bCK3a_TYKI2Pv-hQZHL75jCr3vLIVoU_IutN6eUEs7bDz4PvNDxqmLoaP3-OUhjOmjjQO92yUbzrYJ91Z1hzxfXz1d3hbTh5u7y_NpAUKJoaikrholZ04JyTgga2pdcVdW6JxkswoabgGBNzkrMKgss1rXQjWCO-5kKXfI4fLuewwfc0yD6XwCbFvbY5gnoyomJ6LUGSyXIMSQUkRn3mNOFr8NZ2Zh1yzUmYU6U0vza9dM8trB6v58llP_La105vnZco455KfHaBJ47CELigiDaYL__8EPhtqJYA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76035248</pqid></control><display><type>article</type><title>Pharmacological and chemical screening of Byrsonima crassifolia, a medicinal tree from Mexico. Part I</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Béjar, Ezra ; Malone, Marvin H.</creator><creatorcontrib>Béjar, Ezra ; Malone, Marvin H.</creatorcontrib><description>Leaf and bark extracts of
Byrsonima crassifolia displayed concentration-dependent, spasmogenic effects on rat fundus in vitro and biphasic effects on rat jejunum and ileum in vitro. Dose-related in vivo effects in intact rats using hippocratic screening were: decrease in motor activity, mild analgesia, back tonus, enophthalmos, reversable palpebral ptosis, ear blanching, Robichaud positive, catalepsy (awake) and strong hypothermia. Rat fundus in vitro was used as the bioassay to carry out an activity-directed separation. Bioactive material was concentrated in a 2% acetic acid leaf extract (HOAcE). Potency of HOAcE was increased by the presence of pargyline in the bathing solution. HOAcE was antagonized noncompetively by l(I-naphthyl) piperazine (I-NP) and cyproheptadine and antagonized competitively by atropine (ATR). Cumulative concentration-response curves of HOAcE and serotonin (5-HT) did not show significant departure from parallelism (
P > 0.1) and 5-HT potency was 6040 times that of HOAcE (95% confidence limits: 4620–7850). Solvent extraction of HOAcE split the spasmogenic activity of HOAcE into two types: (i) high-efficacy, low-potency,
n-butanol-extracted, pargyline- and 1-NP-sensitive, ATR-insensitive activity, and (ii) low-efficacy, high-potency, ethyl acetate-extracted, pargyline-insensitive, ATR- and 1-NP-sensitive activity. HOAcE lacked muscarinic and nicotinic effects on rat jejunum and frog rectus abdominis. Results suggest the presence of more than one spasmogenic compound in the plant.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/0378-8741(93)90029-5</identifier><identifier>PMID: 8412247</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Byrsonima crassifolia ; Drug Evaluation, Preclinical ; Gastric Fundus - drug effects ; hippocratic screening ; In Vitro Techniques ; Male ; Medicine, Traditional ; Mexico ; Motor Activity - drug effects ; Muscle Contraction - drug effects ; Muscle, Smooth - drug effects ; Plant Extracts - analysis ; Plant Extracts - pharmacology ; Plants, Medicinal ; rat fundus ; Rats ; Rats, Sprague-Dawley ; serotonin ; spasmogenic activity ; Trees</subject><ispartof>Journal of ethnopharmacology, 1993-06, Vol.39 (2), p.141-158</ispartof><rights>1993</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-6386d73bf72301ce0d9861f46eff30b6cd1acec1d029c0c6a0a88927d21f1f343</citedby><cites>FETCH-LOGICAL-c272t-6386d73bf72301ce0d9861f46eff30b6cd1acec1d029c0c6a0a88927d21f1f343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0378874193900295$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8412247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Béjar, Ezra</creatorcontrib><creatorcontrib>Malone, Marvin H.</creatorcontrib><title>Pharmacological and chemical screening of Byrsonima crassifolia, a medicinal tree from Mexico. Part I</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Leaf and bark extracts of
Byrsonima crassifolia displayed concentration-dependent, spasmogenic effects on rat fundus in vitro and biphasic effects on rat jejunum and ileum in vitro. Dose-related in vivo effects in intact rats using hippocratic screening were: decrease in motor activity, mild analgesia, back tonus, enophthalmos, reversable palpebral ptosis, ear blanching, Robichaud positive, catalepsy (awake) and strong hypothermia. Rat fundus in vitro was used as the bioassay to carry out an activity-directed separation. Bioactive material was concentrated in a 2% acetic acid leaf extract (HOAcE). Potency of HOAcE was increased by the presence of pargyline in the bathing solution. HOAcE was antagonized noncompetively by l(I-naphthyl) piperazine (I-NP) and cyproheptadine and antagonized competitively by atropine (ATR). Cumulative concentration-response curves of HOAcE and serotonin (5-HT) did not show significant departure from parallelism (
P > 0.1) and 5-HT potency was 6040 times that of HOAcE (95% confidence limits: 4620–7850). Solvent extraction of HOAcE split the spasmogenic activity of HOAcE into two types: (i) high-efficacy, low-potency,
n-butanol-extracted, pargyline- and 1-NP-sensitive, ATR-insensitive activity, and (ii) low-efficacy, high-potency, ethyl acetate-extracted, pargyline-insensitive, ATR- and 1-NP-sensitive activity. HOAcE lacked muscarinic and nicotinic effects on rat jejunum and frog rectus abdominis. Results suggest the presence of more than one spasmogenic compound in the plant.</description><subject>Animals</subject><subject>Byrsonima crassifolia</subject><subject>Drug Evaluation, Preclinical</subject><subject>Gastric Fundus - drug effects</subject><subject>hippocratic screening</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Medicine, Traditional</subject><subject>Mexico</subject><subject>Motor Activity - drug effects</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth - drug effects</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - pharmacology</subject><subject>Plants, Medicinal</subject><subject>rat fundus</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>serotonin</subject><subject>spasmogenic activity</subject><subject>Trees</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78A4WcRMGu-eg26UVQ8QtW9KDnkJ1ONNI2mnRF_71Zd_HoaRjmmRneh5B9zsac8eqUSaULrUp-VMvjmjFRF5M1MuJaiUJNlFwnoz9ki2yn9MYYU7xkm2RTl1yIUo0IPr7a2FkIbXjxYFtq-4bCK3a_TYKI2Pv-hQZHL75jCr3vLIVoU_IutN6eUEs7bDz4PvNDxqmLoaP3-OUhjOmjjQO92yUbzrYJ91Z1hzxfXz1d3hbTh5u7y_NpAUKJoaikrholZ04JyTgga2pdcVdW6JxkswoabgGBNzkrMKgss1rXQjWCO-5kKXfI4fLuewwfc0yD6XwCbFvbY5gnoyomJ6LUGSyXIMSQUkRn3mNOFr8NZ2Zh1yzUmYU6U0vza9dM8trB6v58llP_La105vnZco455KfHaBJ47CELigiDaYL__8EPhtqJYA</recordid><startdate>199306</startdate><enddate>199306</enddate><creator>Béjar, Ezra</creator><creator>Malone, Marvin H.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199306</creationdate><title>Pharmacological and chemical screening of Byrsonima crassifolia, a medicinal tree from Mexico. Part I</title><author>Béjar, Ezra ; Malone, Marvin H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-6386d73bf72301ce0d9861f46eff30b6cd1acec1d029c0c6a0a88927d21f1f343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Byrsonima crassifolia</topic><topic>Drug Evaluation, Preclinical</topic><topic>Gastric Fundus - drug effects</topic><topic>hippocratic screening</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Medicine, Traditional</topic><topic>Mexico</topic><topic>Motor Activity - drug effects</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth - drug effects</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - pharmacology</topic><topic>Plants, Medicinal</topic><topic>rat fundus</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>serotonin</topic><topic>spasmogenic activity</topic><topic>Trees</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Béjar, Ezra</creatorcontrib><creatorcontrib>Malone, Marvin H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Béjar, Ezra</au><au>Malone, Marvin H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pharmacological and chemical screening of Byrsonima crassifolia, a medicinal tree from Mexico. Part I</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>1993-06</date><risdate>1993</risdate><volume>39</volume><issue>2</issue><spage>141</spage><epage>158</epage><pages>141-158</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Leaf and bark extracts of
Byrsonima crassifolia displayed concentration-dependent, spasmogenic effects on rat fundus in vitro and biphasic effects on rat jejunum and ileum in vitro. Dose-related in vivo effects in intact rats using hippocratic screening were: decrease in motor activity, mild analgesia, back tonus, enophthalmos, reversable palpebral ptosis, ear blanching, Robichaud positive, catalepsy (awake) and strong hypothermia. Rat fundus in vitro was used as the bioassay to carry out an activity-directed separation. Bioactive material was concentrated in a 2% acetic acid leaf extract (HOAcE). Potency of HOAcE was increased by the presence of pargyline in the bathing solution. HOAcE was antagonized noncompetively by l(I-naphthyl) piperazine (I-NP) and cyproheptadine and antagonized competitively by atropine (ATR). Cumulative concentration-response curves of HOAcE and serotonin (5-HT) did not show significant departure from parallelism (
P > 0.1) and 5-HT potency was 6040 times that of HOAcE (95% confidence limits: 4620–7850). Solvent extraction of HOAcE split the spasmogenic activity of HOAcE into two types: (i) high-efficacy, low-potency,
n-butanol-extracted, pargyline- and 1-NP-sensitive, ATR-insensitive activity, and (ii) low-efficacy, high-potency, ethyl acetate-extracted, pargyline-insensitive, ATR- and 1-NP-sensitive activity. HOAcE lacked muscarinic and nicotinic effects on rat jejunum and frog rectus abdominis. Results suggest the presence of more than one spasmogenic compound in the plant.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>8412247</pmid><doi>10.1016/0378-8741(93)90029-5</doi><tpages>18</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-8741 |
| ispartof | Journal of ethnopharmacology, 1993-06, Vol.39 (2), p.141-158 |
| issn | 0378-8741 1872-7573 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76035248 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Byrsonima crassifolia Drug Evaluation, Preclinical Gastric Fundus - drug effects hippocratic screening In Vitro Techniques Male Medicine, Traditional Mexico Motor Activity - drug effects Muscle Contraction - drug effects Muscle, Smooth - drug effects Plant Extracts - analysis Plant Extracts - pharmacology Plants, Medicinal rat fundus Rats Rats, Sprague-Dawley serotonin spasmogenic activity Trees |
| title | Pharmacological and chemical screening of Byrsonima crassifolia, a medicinal tree from Mexico. Part I |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T00%3A31%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pharmacological%20and%20chemical%20screening%20of%20Byrsonima%20crassifolia,%20a%20medicinal%20tree%20from%20Mexico.%20Part%20I&rft.jtitle=Journal%20of%20ethnopharmacology&rft.au=B%C3%A9jar,%20Ezra&rft.date=1993-06&rft.volume=39&rft.issue=2&rft.spage=141&rft.epage=158&rft.pages=141-158&rft.issn=0378-8741&rft.eissn=1872-7573&rft_id=info:doi/10.1016/0378-8741(93)90029-5&rft_dat=%3Cproquest_cross%3E76035248%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76035248&rft_id=info:pmid/8412247&rft_els_id=0378874193900295&rfr_iscdi=true |