New insights suggesting a possible role of a heat shock protein 70-kD family-related protein in antigen processing/presentation phenomenon in humans
A possible role of the peptide binding protein (PBP) 72/74 in antigen processing and presentation has been recently suggested in mice. In order to evaluate a possible analogous role of a PBP72/74-related protein in humans, immunoelectron microscope investigations, functional studies, and immunofluor...
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| Veröffentlicht in: | Blood 1993-11, Vol.82 (9), p.2865-2871 |
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| creator | MANARA, G. C SANSONI, P BADIALI-DE GIORGI, L GALLINELLA, G FERRARI, C BRIANTI, V FAGNONI, F. F RUEGG, C. L DE PANFILIS, G PASQUINELLI, G |
| description | A possible role of the peptide binding protein (PBP) 72/74 in antigen processing and presentation has been recently suggested in mice. In order to evaluate a possible analogous role of a PBP72/74-related protein in humans, immunoelectron microscope investigations, functional studies, and immunofluorescence analyses were performed on normal human peripheral antigen-presenting cells. We demonstrated that the determinant recognized by antiheat shock protein (HSP) 72/73 monoclonal antibody (MoAb) is constitutively expressed on the cell surface of monocytes as well as of B cells. Moreover, the capability of monocytes to present a recall antigen to T cells was significantly decreased when preincubated with an anti-HSP72/73 MoAb. These data add further strength to a potential role of a protein related to human PBP72/74 homologue in antigen processing and/or presentation. Finally, the capability of anti-HSP72/73 MoAb to impair the ability of fixed monocytes to present a synthetic peptide demonstrates that cell surface-localized PBP72/74-related protein could play a role in antigen presentation. |
| doi_str_mv | 10.1182/blood.V82.9.2865.2865 |
| format | Article |
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C ; SANSONI, P ; BADIALI-DE GIORGI, L ; GALLINELLA, G ; FERRARI, C ; BRIANTI, V ; FAGNONI, F. F ; RUEGG, C. L ; DE PANFILIS, G ; PASQUINELLI, G</creator><creatorcontrib>MANARA, G. C ; SANSONI, P ; BADIALI-DE GIORGI, L ; GALLINELLA, G ; FERRARI, C ; BRIANTI, V ; FAGNONI, F. F ; RUEGG, C. L ; DE PANFILIS, G ; PASQUINELLI, G</creatorcontrib><description>A possible role of the peptide binding protein (PBP) 72/74 in antigen processing and presentation has been recently suggested in mice. In order to evaluate a possible analogous role of a PBP72/74-related protein in humans, immunoelectron microscope investigations, functional studies, and immunofluorescence analyses were performed on normal human peripheral antigen-presenting cells. We demonstrated that the determinant recognized by antiheat shock protein (HSP) 72/73 monoclonal antibody (MoAb) is constitutively expressed on the cell surface of monocytes as well as of B cells. Moreover, the capability of monocytes to present a recall antigen to T cells was significantly decreased when preincubated with an anti-HSP72/73 MoAb. These data add further strength to a potential role of a protein related to human PBP72/74 homologue in antigen processing and/or presentation. Finally, the capability of anti-HSP72/73 MoAb to impair the ability of fixed monocytes to present a synthetic peptide demonstrates that cell surface-localized PBP72/74-related protein could play a role in antigen presentation.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V82.9.2865.2865</identifier><identifier>PMID: 8219234</identifier><language>eng</language><publisher>Washington, DC: The Americain Society of Hematology</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Antibodies, Monoclonal - immunology ; Antigen Presentation ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Heat-Shock Proteins - physiology ; HLA-DR Antigens - physiology ; Humans ; Immunobiology ; Lymphocyte Activation ; Miscellaneous ; Molecular Weight ; Monocytes - immunology ; Monocytes - ultrastructure ; Regulatory factors and their cellular receptors ; Tetanus Toxoid - pharmacology</subject><ispartof>Blood, 1993-11, Vol.82 (9), p.2865-2871</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-b9338b9ff9f83977a1234493fc9aca970759156fe5b9ab0495d7af64e6eafae3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3823501$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8219234$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MANARA, G. C</creatorcontrib><creatorcontrib>SANSONI, P</creatorcontrib><creatorcontrib>BADIALI-DE GIORGI, L</creatorcontrib><creatorcontrib>GALLINELLA, G</creatorcontrib><creatorcontrib>FERRARI, C</creatorcontrib><creatorcontrib>BRIANTI, V</creatorcontrib><creatorcontrib>FAGNONI, F. F</creatorcontrib><creatorcontrib>RUEGG, C. L</creatorcontrib><creatorcontrib>DE PANFILIS, G</creatorcontrib><creatorcontrib>PASQUINELLI, G</creatorcontrib><title>New insights suggesting a possible role of a heat shock protein 70-kD family-related protein in antigen processing/presentation phenomenon in humans</title><title>Blood</title><addtitle>Blood</addtitle><description>A possible role of the peptide binding protein (PBP) 72/74 in antigen processing and presentation has been recently suggested in mice. In order to evaluate a possible analogous role of a PBP72/74-related protein in humans, immunoelectron microscope investigations, functional studies, and immunofluorescence analyses were performed on normal human peripheral antigen-presenting cells. We demonstrated that the determinant recognized by antiheat shock protein (HSP) 72/73 monoclonal antibody (MoAb) is constitutively expressed on the cell surface of monocytes as well as of B cells. Moreover, the capability of monocytes to present a recall antigen to T cells was significantly decreased when preincubated with an anti-HSP72/73 MoAb. These data add further strength to a potential role of a protein related to human PBP72/74 homologue in antigen processing and/or presentation. Finally, the capability of anti-HSP72/73 MoAb to impair the ability of fixed monocytes to present a synthetic peptide demonstrates that cell surface-localized PBP72/74-related protein could play a role in antigen presentation.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigen Presentation</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Heat-Shock Proteins - physiology</subject><subject>HLA-DR Antigens - physiology</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Lymphocyte Activation</subject><subject>Miscellaneous</subject><subject>Molecular Weight</subject><subject>Monocytes - immunology</subject><subject>Monocytes - ultrastructure</subject><subject>Regulatory factors and their cellular receptors</subject><subject>Tetanus Toxoid - pharmacology</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UctuFDEQtBAoLAmfEMkHxG02fszDPqJAACmCS5Sr1eNtz5jM2MvYI5T_4IPj3axWsrulquq2u4uQa862nCtx008x7raPSmz1Vqi2OYY3ZMMboSrGBHtLNoyxtqp1x9-TDyn9YYzXUjQX5EIJroWsN-T_L_xHfUh-GHOiaR0GTNmHgQLdx5R8PyFdYgnRFWhEyDSN0T7R_RIz-kA7Vj19pQ5mPz1XC06QcXcmy4GQ_YDhAFksDcNws18wYciQfSz4iCHO5R7V4zpDSFfknYMp4cdTviQPd98ebn9U97-__7z9cl9ZqViuei2l6rVz2impuw54GanW0lkNFnTHukbzpnXY9Bp6Vutm14Fra2wRHKC8JJ9f25a__V3L3Gb2yeI0QcC4JtO1TNatqouweRXapexkQWf2i59heTacmYMZ5miGKWYYbQ4-HEOpuz49sPYz7s5Vp-0X_tOJh2RhcgsE69NZJpWQDePyBbFWl2g</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>MANARA, G. C</creator><creator>SANSONI, P</creator><creator>BADIALI-DE GIORGI, L</creator><creator>GALLINELLA, G</creator><creator>FERRARI, C</creator><creator>BRIANTI, V</creator><creator>FAGNONI, F. F</creator><creator>RUEGG, C. L</creator><creator>DE PANFILIS, G</creator><creator>PASQUINELLI, G</creator><general>The Americain Society of Hematology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931101</creationdate><title>New insights suggesting a possible role of a heat shock protein 70-kD family-related protein in antigen processing/presentation phenomenon in humans</title><author>MANARA, G. C ; SANSONI, P ; BADIALI-DE GIORGI, L ; GALLINELLA, G ; FERRARI, C ; BRIANTI, V ; FAGNONI, F. F ; RUEGG, C. L ; DE PANFILIS, G ; PASQUINELLI, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-b9338b9ff9f83977a1234493fc9aca970759156fe5b9ab0495d7af64e6eafae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigen Presentation</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Heat-Shock Proteins - physiology</topic><topic>HLA-DR Antigens - physiology</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Lymphocyte Activation</topic><topic>Miscellaneous</topic><topic>Molecular Weight</topic><topic>Monocytes - immunology</topic><topic>Monocytes - ultrastructure</topic><topic>Regulatory factors and their cellular receptors</topic><topic>Tetanus Toxoid - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MANARA, G. C</creatorcontrib><creatorcontrib>SANSONI, P</creatorcontrib><creatorcontrib>BADIALI-DE GIORGI, L</creatorcontrib><creatorcontrib>GALLINELLA, G</creatorcontrib><creatorcontrib>FERRARI, C</creatorcontrib><creatorcontrib>BRIANTI, V</creatorcontrib><creatorcontrib>FAGNONI, F. F</creatorcontrib><creatorcontrib>RUEGG, C. L</creatorcontrib><creatorcontrib>DE PANFILIS, G</creatorcontrib><creatorcontrib>PASQUINELLI, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MANARA, G. C</au><au>SANSONI, P</au><au>BADIALI-DE GIORGI, L</au><au>GALLINELLA, G</au><au>FERRARI, C</au><au>BRIANTI, V</au><au>FAGNONI, F. F</au><au>RUEGG, C. L</au><au>DE PANFILIS, G</au><au>PASQUINELLI, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New insights suggesting a possible role of a heat shock protein 70-kD family-related protein in antigen processing/presentation phenomenon in humans</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>82</volume><issue>9</issue><spage>2865</spage><epage>2871</epage><pages>2865-2871</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>A possible role of the peptide binding protein (PBP) 72/74 in antigen processing and presentation has been recently suggested in mice. In order to evaluate a possible analogous role of a PBP72/74-related protein in humans, immunoelectron microscope investigations, functional studies, and immunofluorescence analyses were performed on normal human peripheral antigen-presenting cells. We demonstrated that the determinant recognized by antiheat shock protein (HSP) 72/73 monoclonal antibody (MoAb) is constitutively expressed on the cell surface of monocytes as well as of B cells. Moreover, the capability of monocytes to present a recall antigen to T cells was significantly decreased when preincubated with an anti-HSP72/73 MoAb. These data add further strength to a potential role of a protein related to human PBP72/74 homologue in antigen processing and/or presentation. Finally, the capability of anti-HSP72/73 MoAb to impair the ability of fixed monocytes to present a synthetic peptide demonstrates that cell surface-localized PBP72/74-related protein could play a role in antigen presentation.</abstract><cop>Washington, DC</cop><pub>The Americain Society of Hematology</pub><pmid>8219234</pmid><doi>10.1182/blood.V82.9.2865.2865</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| subjects | Analysis of the immune response. Humoral and cellular immunity Antibodies, Monoclonal - immunology Antigen Presentation Biological and medical sciences Fundamental and applied biological sciences. Psychology Fundamental immunology Heat-Shock Proteins - physiology HLA-DR Antigens - physiology Humans Immunobiology Lymphocyte Activation Miscellaneous Molecular Weight Monocytes - immunology Monocytes - ultrastructure Regulatory factors and their cellular receptors Tetanus Toxoid - pharmacology |
| title | New insights suggesting a possible role of a heat shock protein 70-kD family-related protein in antigen processing/presentation phenomenon in humans |
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