Insulin Gene Can Be Expressed in the Absence of Isl-1
The possible role of Isl-1 in insulin-gene transcription was investigated using Northern blot analysis to determine whether insulin and Isl-1 gene expression are correlated in various somatic cell hybrids. Among several hybrid cell lines obtained by fusing insulin-producing rat insulinoma (RIN) cell...
Gespeichert in:
| Veröffentlicht in: | Experimental cell research 1993-11, Vol.209 (1), p.58-63 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 63 |
|---|---|
| container_issue | 1 |
| container_start_page | 58 |
| container_title | Experimental cell research |
| container_volume | 209 |
| creator | Dandoy-Dron, Francoise Deltour, Louise Monthioux, Eliane Bucchini, Danielle Jami, Jacques |
| description | The possible role of Isl-1 in insulin-gene transcription was investigated using Northern blot analysis to determine whether insulin and Isl-1 gene expression are correlated in various somatic cell hybrids. Among several hybrid cell lines obtained by fusing insulin-producing rat insulinoma (RIN) cells and mouse spleen cells, three (RR2, RR5, and RR11) had amounts of insulin transcripts similar to those of the parental RIN cells, although two of them, RR5 and RR11, lacked Isl-1 protein. In contrast, two R1N × mouse L cell hybrids where insulin expression was extinct still expressed Isl-1. RT-PCR analysis showed that Isl-1 transcripts are widely distributed in various mouse tissues, including pancreas, brain, lung, thymus, and ovary. These results indicate that Is-1 is not essential for insulin gene expression. |
| doi_str_mv | 10.1006/excr.1993.1285 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76032271</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014482783712851</els_id><sourcerecordid>76032271</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-a1e725e4f7655b0caf9ff0e11b031122abe1c208021c7fd5dcb53351bff15f303</originalsourceid><addsrcrecordid>eNp1kDFPwzAQhS0EKqWwsiFlQGwJd3acuGOpSqlUiQVmy3HOIihNip2i8u9J1IiN6Yb3vafTx9gtQoIA2SMdrU9wPhcJciXP2BRhDjFPOT9nUwBM41Tx_JJdhfAJAEphNmETxXkKkE-Z3DThUFdNtKaGoqVpoieKVse9pxCojPqg-6BoUQRqLEWtizahjvGaXThTB7oZ74y9P6_eli_x9nW9WS62sRWZ6mKDlHNJqcszKQuwxs2dA0IsQCBybgpCy0EBR5u7Upa2kEJILJxD6QSIGXs47e59-3Wg0OldFSzVtWmoPQSdZyA4z7EHkxNofRuCJ6f3vtoZ_6MR9OBJD5704EkPnvrC3bh8KHZU_uGjmD6_H3MTrKmdN42twh8mFIgMsh5TJ4x6C98VeR1sNagqK0-202Vb_ffBLz53gQU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76032271</pqid></control><display><type>article</type><title>Insulin Gene Can Be Expressed in the Absence of Isl-1</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Dandoy-Dron, Francoise ; Deltour, Louise ; Monthioux, Eliane ; Bucchini, Danielle ; Jami, Jacques</creator><creatorcontrib>Dandoy-Dron, Francoise ; Deltour, Louise ; Monthioux, Eliane ; Bucchini, Danielle ; Jami, Jacques</creatorcontrib><description>The possible role of Isl-1 in insulin-gene transcription was investigated using Northern blot analysis to determine whether insulin and Isl-1 gene expression are correlated in various somatic cell hybrids. Among several hybrid cell lines obtained by fusing insulin-producing rat insulinoma (RIN) cells and mouse spleen cells, three (RR2, RR5, and RR11) had amounts of insulin transcripts similar to those of the parental RIN cells, although two of them, RR5 and RR11, lacked Isl-1 protein. In contrast, two R1N × mouse L cell hybrids where insulin expression was extinct still expressed Isl-1. RT-PCR analysis showed that Isl-1 transcripts are widely distributed in various mouse tissues, including pancreas, brain, lung, thymus, and ovary. These results indicate that Is-1 is not essential for insulin gene expression.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1006/excr.1993.1285</identifier><identifier>PMID: 8224007</identifier><identifier>CODEN: ECREAL</identifier><language>eng</language><publisher>Orlando, FL: Elsevier Inc</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Blotting, Western ; Cell Line ; DNA-Binding Proteins - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Homeodomain Proteins ; Hybrid Cells ; Insulin - genetics ; Insulinoma - genetics ; L Cells (Cell Line) ; LIM-Homeodomain Proteins ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nerve Tissue Proteins ; Rats ; Spleen ; Transcription Factors ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>Experimental cell research, 1993-11, Vol.209 (1), p.58-63</ispartof><rights>1993 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-a1e725e4f7655b0caf9ff0e11b031122abe1c208021c7fd5dcb53351bff15f303</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014482783712851$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3803606$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8224007$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dandoy-Dron, Francoise</creatorcontrib><creatorcontrib>Deltour, Louise</creatorcontrib><creatorcontrib>Monthioux, Eliane</creatorcontrib><creatorcontrib>Bucchini, Danielle</creatorcontrib><creatorcontrib>Jami, Jacques</creatorcontrib><title>Insulin Gene Can Be Expressed in the Absence of Isl-1</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>The possible role of Isl-1 in insulin-gene transcription was investigated using Northern blot analysis to determine whether insulin and Isl-1 gene expression are correlated in various somatic cell hybrids. Among several hybrid cell lines obtained by fusing insulin-producing rat insulinoma (RIN) cells and mouse spleen cells, three (RR2, RR5, and RR11) had amounts of insulin transcripts similar to those of the parental RIN cells, although two of them, RR5 and RR11, lacked Isl-1 protein. In contrast, two R1N × mouse L cell hybrids where insulin expression was extinct still expressed Isl-1. RT-PCR analysis showed that Isl-1 transcripts are widely distributed in various mouse tissues, including pancreas, brain, lung, thymus, and ovary. These results indicate that Is-1 is not essential for insulin gene expression.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>DNA-Binding Proteins - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Homeodomain Proteins</subject><subject>Hybrid Cells</subject><subject>Insulin - genetics</subject><subject>Insulinoma - genetics</subject><subject>L Cells (Cell Line)</subject><subject>LIM-Homeodomain Proteins</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nerve Tissue Proteins</subject><subject>Rats</subject><subject>Spleen</subject><subject>Transcription Factors</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDFPwzAQhS0EKqWwsiFlQGwJd3acuGOpSqlUiQVmy3HOIihNip2i8u9J1IiN6Yb3vafTx9gtQoIA2SMdrU9wPhcJciXP2BRhDjFPOT9nUwBM41Tx_JJdhfAJAEphNmETxXkKkE-Z3DThUFdNtKaGoqVpoieKVse9pxCojPqg-6BoUQRqLEWtizahjvGaXThTB7oZ74y9P6_eli_x9nW9WS62sRWZ6mKDlHNJqcszKQuwxs2dA0IsQCBybgpCy0EBR5u7Upa2kEJILJxD6QSIGXs47e59-3Wg0OldFSzVtWmoPQSdZyA4z7EHkxNofRuCJ6f3vtoZ_6MR9OBJD5704EkPnvrC3bh8KHZU_uGjmD6_H3MTrKmdN42twh8mFIgMsh5TJ4x6C98VeR1sNagqK0-202Vb_ffBLz53gQU</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>Dandoy-Dron, Francoise</creator><creator>Deltour, Louise</creator><creator>Monthioux, Eliane</creator><creator>Bucchini, Danielle</creator><creator>Jami, Jacques</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931101</creationdate><title>Insulin Gene Can Be Expressed in the Absence of Isl-1</title><author>Dandoy-Dron, Francoise ; Deltour, Louise ; Monthioux, Eliane ; Bucchini, Danielle ; Jami, Jacques</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-a1e725e4f7655b0caf9ff0e11b031122abe1c208021c7fd5dcb53351bff15f303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>DNA-Binding Proteins - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Homeodomain Proteins</topic><topic>Hybrid Cells</topic><topic>Insulin - genetics</topic><topic>Insulinoma - genetics</topic><topic>L Cells (Cell Line)</topic><topic>LIM-Homeodomain Proteins</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nerve Tissue Proteins</topic><topic>Rats</topic><topic>Spleen</topic><topic>Transcription Factors</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dandoy-Dron, Francoise</creatorcontrib><creatorcontrib>Deltour, Louise</creatorcontrib><creatorcontrib>Monthioux, Eliane</creatorcontrib><creatorcontrib>Bucchini, Danielle</creatorcontrib><creatorcontrib>Jami, Jacques</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dandoy-Dron, Francoise</au><au>Deltour, Louise</au><au>Monthioux, Eliane</au><au>Bucchini, Danielle</au><au>Jami, Jacques</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insulin Gene Can Be Expressed in the Absence of Isl-1</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>209</volume><issue>1</issue><spage>58</spage><epage>63</epage><pages>58-63</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>The possible role of Isl-1 in insulin-gene transcription was investigated using Northern blot analysis to determine whether insulin and Isl-1 gene expression are correlated in various somatic cell hybrids. Among several hybrid cell lines obtained by fusing insulin-producing rat insulinoma (RIN) cells and mouse spleen cells, three (RR2, RR5, and RR11) had amounts of insulin transcripts similar to those of the parental RIN cells, although two of them, RR5 and RR11, lacked Isl-1 protein. In contrast, two R1N × mouse L cell hybrids where insulin expression was extinct still expressed Isl-1. RT-PCR analysis showed that Isl-1 transcripts are widely distributed in various mouse tissues, including pancreas, brain, lung, thymus, and ovary. These results indicate that Is-1 is not essential for insulin gene expression.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>8224007</pmid><doi>10.1006/excr.1993.1285</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4827 |
| ispartof | Experimental cell research, 1993-11, Vol.209 (1), p.58-63 |
| issn | 0014-4827 1090-2422 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76032271 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Base Sequence Biological and medical sciences Blotting, Northern Blotting, Western Cell Line DNA-Binding Proteins - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression Regulation Homeodomain Proteins Hybrid Cells Insulin - genetics Insulinoma - genetics L Cells (Cell Line) LIM-Homeodomain Proteins Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data Nerve Tissue Proteins Rats Spleen Transcription Factors Transcription. Transcription factor. Splicing. Rna processing |
| title | Insulin Gene Can Be Expressed in the Absence of Isl-1 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T09%3A35%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Insulin%20Gene%20Can%20Be%20Expressed%20in%20the%20Absence%20of%20Isl-1&rft.jtitle=Experimental%20cell%20research&rft.au=Dandoy-Dron,%20Francoise&rft.date=1993-11-01&rft.volume=209&rft.issue=1&rft.spage=58&rft.epage=63&rft.pages=58-63&rft.issn=0014-4827&rft.eissn=1090-2422&rft.coden=ECREAL&rft_id=info:doi/10.1006/excr.1993.1285&rft_dat=%3Cproquest_cross%3E76032271%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76032271&rft_id=info:pmid/8224007&rft_els_id=S0014482783712851&rfr_iscdi=true |