Hormonal Control of Glycogenolysis in Isolated Chick Embryo Hepatocytes
Hepatocytes were isolated from 15-, 16-, 17-, and 18day-old chick embryos. Glucagon, adrenaline, and dibutyryl cAMP (Bt2cAMP), individually or in combination, activated glycogenolysis in the isolated hepatocytes. The α-adrenergic agonist phenylephrine did not increase glycogen breakdown. The action...
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| Veröffentlicht in: | Experimental cell research 1993-11, Vol.209 (1), p.1-5 |
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| creator | Onoagbe, I.O. |
| description | Hepatocytes were isolated from 15-, 16-, 17-, and 18day-old chick embryos. Glucagon, adrenaline, and dibutyryl cAMP (Bt2cAMP), individually or in combination, activated glycogenolysis in the isolated hepatocytes. The α-adrenergic agonist phenylephrine did not increase glycogen breakdown. The action of adrenaline was abolished upon treatment of hepatocytes with a combination of the hormone and propranolol, a β-adrenergic blocker. The effects of glucagon, adrenaline, and dibutyryl cAMP on glycogenolysis were not additive. Either hormone induced an increase in the concentration of cAMP. The activities of dephosphophosphorylase kinase and phosphorylase a were stimulated by each hormone or Bt2cAMP. It appears likely that glucagon and adrenaline serve as physiological regulators of hepatic glycogen breakdown during embryogenesis in chickens. |
| doi_str_mv | 10.1006/excr.1993.1277 |
| format | Article |
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Glucagon, adrenaline, and dibutyryl cAMP (Bt2cAMP), individually or in combination, activated glycogenolysis in the isolated hepatocytes. The α-adrenergic agonist phenylephrine did not increase glycogen breakdown. The action of adrenaline was abolished upon treatment of hepatocytes with a combination of the hormone and propranolol, a β-adrenergic blocker. The effects of glucagon, adrenaline, and dibutyryl cAMP on glycogenolysis were not additive. Either hormone induced an increase in the concentration of cAMP. The activities of dephosphophosphorylase kinase and phosphorylase a were stimulated by each hormone or Bt2cAMP. It appears likely that glucagon and adrenaline serve as physiological regulators of hepatic glycogen breakdown during embryogenesis in chickens.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1006/excr.1993.1277</identifier><identifier>PMID: 8223994</identifier><identifier>CODEN: ECREAL</identifier><language>eng</language><publisher>Orlando, FL: Elsevier Inc</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; Biological and medical sciences ; Bucladesine - pharmacology ; Cell Survival ; Chick Embryo ; Embryology: invertebrates and vertebrates. Teratology ; Epinephrine - pharmacology ; Fundamental and applied biological sciences. Psychology ; Glucagon - pharmacology ; Glucose - metabolism ; L-Lactate Dehydrogenase - metabolism ; Liver - embryology ; Liver - metabolism ; Liver Glycogen - metabolism ; Organogenesis. Physiological fonctions ; Phenylephrine - pharmacology ; Phosphorylase a - metabolism ; Physiological fonctions ; Propranolol - pharmacology</subject><ispartof>Experimental cell research, 1993-11, Vol.209 (1), p.1-5</ispartof><rights>1993 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-498a5395e258d8d86928968ec8725ab6d116549a44060b2da83a2de04243cf3b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/excr.1993.1277$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3803598$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8223994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Onoagbe, I.O.</creatorcontrib><title>Hormonal Control of Glycogenolysis in Isolated Chick Embryo Hepatocytes</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Hepatocytes were isolated from 15-, 16-, 17-, and 18day-old chick embryos. Glucagon, adrenaline, and dibutyryl cAMP (Bt2cAMP), individually or in combination, activated glycogenolysis in the isolated hepatocytes. The α-adrenergic agonist phenylephrine did not increase glycogen breakdown. The action of adrenaline was abolished upon treatment of hepatocytes with a combination of the hormone and propranolol, a β-adrenergic blocker. The effects of glucagon, adrenaline, and dibutyryl cAMP on glycogenolysis were not additive. Either hormone induced an increase in the concentration of cAMP. The activities of dephosphophosphorylase kinase and phosphorylase a were stimulated by each hormone or Bt2cAMP. It appears likely that glucagon and adrenaline serve as physiological regulators of hepatic glycogen breakdown during embryogenesis in chickens.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bucladesine - pharmacology</subject><subject>Cell Survival</subject><subject>Chick Embryo</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Epinephrine - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucagon - pharmacology</subject><subject>Glucose - metabolism</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Liver - embryology</subject><subject>Liver - metabolism</subject><subject>Liver Glycogen - metabolism</subject><subject>Organogenesis. Physiological fonctions</subject><subject>Phenylephrine - pharmacology</subject><subject>Phosphorylase a - metabolism</subject><subject>Physiological fonctions</subject><subject>Propranolol - pharmacology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1v2zAQhomiQeomWbsV0FB0k3L8EEWOhZHYBgJkSWaCok4tW0p0SbmI_n1l2PAW3HDD-9yLw0PIFwoVBZD3-OZSRbXmFWVN84GsKGgomWDsI1kBUFEKxZpP5HPOvwFAKSqvybVijGstVmSzjWmIow3FOo5TiqGIfbEJs4s_cYxhzj4Xfix2OQY7YVesf3n3p3gY2jTHYot7O0U3T5hvyVVvQ8a7874hr48PL-tt-fS82a1_PJWOSzWVQitbc10jq1W3jNRMaanQqYbVtpUdpbIW2goBElrWWcUt6xAEE9z1vOU35Pupd5_i3wPmyQw-OwzBjhgP2TQSOKMNLGB1Al2KOSfszT75wabZUDBHc-ZozhzNmaO55eDrufnQDthd8LOqJf92zm12NvTJjs7nC8YV8FqrBVMnDBcL_zwmk53H0WHnE7rJdNG_98F_CtGI0w</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>Onoagbe, I.O.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931101</creationdate><title>Hormonal Control of Glycogenolysis in Isolated Chick Embryo Hepatocytes</title><author>Onoagbe, I.O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-498a5395e258d8d86928968ec8725ab6d116549a44060b2da83a2de04243cf3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bucladesine - pharmacology</topic><topic>Cell Survival</topic><topic>Chick Embryo</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Epinephrine - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucagon - pharmacology</topic><topic>Glucose - metabolism</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Liver - embryology</topic><topic>Liver - metabolism</topic><topic>Liver Glycogen - metabolism</topic><topic>Organogenesis. Physiological fonctions</topic><topic>Phenylephrine - pharmacology</topic><topic>Phosphorylase a - metabolism</topic><topic>Physiological fonctions</topic><topic>Propranolol - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Onoagbe, I.O.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Onoagbe, I.O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hormonal Control of Glycogenolysis in Isolated Chick Embryo Hepatocytes</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>209</volume><issue>1</issue><spage>1</spage><epage>5</epage><pages>1-5</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>Hepatocytes were isolated from 15-, 16-, 17-, and 18day-old chick embryos. Glucagon, adrenaline, and dibutyryl cAMP (Bt2cAMP), individually or in combination, activated glycogenolysis in the isolated hepatocytes. The α-adrenergic agonist phenylephrine did not increase glycogen breakdown. The action of adrenaline was abolished upon treatment of hepatocytes with a combination of the hormone and propranolol, a β-adrenergic blocker. The effects of glucagon, adrenaline, and dibutyryl cAMP on glycogenolysis were not additive. Either hormone induced an increase in the concentration of cAMP. The activities of dephosphophosphorylase kinase and phosphorylase a were stimulated by each hormone or Bt2cAMP. It appears likely that glucagon and adrenaline serve as physiological regulators of hepatic glycogen breakdown during embryogenesis in chickens.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>8223994</pmid><doi>10.1006/excr.1993.1277</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Experimental cell research, 1993-11, Vol.209 (1), p.1-5 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphate - metabolism Animals Biological and medical sciences Bucladesine - pharmacology Cell Survival Chick Embryo Embryology: invertebrates and vertebrates. Teratology Epinephrine - pharmacology Fundamental and applied biological sciences. Psychology Glucagon - pharmacology Glucose - metabolism L-Lactate Dehydrogenase - metabolism Liver - embryology Liver - metabolism Liver Glycogen - metabolism Organogenesis. Physiological fonctions Phenylephrine - pharmacology Phosphorylase a - metabolism Physiological fonctions Propranolol - pharmacology |
| title | Hormonal Control of Glycogenolysis in Isolated Chick Embryo Hepatocytes |
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