Synthetic peptides as antigens: Pitfalls of conjugation methods
Peptide-carrier conjugates were prepared using 9 different synthetic peptides, 3 carrier proteins and 4 coupling reagents. Residues of the carrier protein that were modified by different coupling reagents (e.g., glutaraldehyde, carbodiimides, bis-diazotized benzidine) were found to elicit specific a...
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| Veröffentlicht in: | Journal of immunological methods 1985-04, Vol.78 (1), p.59-69 |
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| container_end_page | 69 |
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| container_title | Journal of immunological methods |
| container_volume | 78 |
| creator | Briand, J.P. Muller, S. Van Regenmortel, M.H.V. |
| description | Peptide-carrier conjugates were prepared using 9 different synthetic peptides, 3 carrier proteins and 4 coupling reagents. Residues of the carrier protein that were modified by different coupling reagents (e.g., glutaraldehyde, carbodiimides, bis-diazotized benzidine) were found to elicit specific antibodies that reacted with unrelated carrier proteins treated with the same coupling agent. To demonstrate the presence of peptide antibodies in an antiserum raised against a peptide-carrier conjugate, it was necessary to use as antigen the peptide coupled to another carrier by means of a different coupling agent.
Some of the commonly used conjugation methods were found to lead to conjugates of insufficient stability and sometimes also altered the antigenic properties of the peptide moiety. These difficulties can be overcome by additional control experiments designed to test the quality and the peptide-carrier conjugates. |
| doi_str_mv | 10.1016/0022-1759(85)90329-1 |
| format | Article |
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Some of the commonly used conjugation methods were found to lead to conjugates of insufficient stability and sometimes also altered the antigenic properties of the peptide moiety. These difficulties can be overcome by additional control experiments designed to test the quality and the peptide-carrier conjugates.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(85)90329-1</identifier><identifier>PMID: 2580027</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Antigenic determinants, haptens, artificial antigens ; Antigens ; Biological and medical sciences ; bis-diazotized benzidine ; carbodiimides ; Carrier Proteins - immunology ; Chemical Phenomena ; Chemistry ; coupling procedures ; Epitopes ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; glutaraldehyde ; Immunosorbent Techniques ; Molecular immunology ; Oligopeptides - chemical synthesis ; Oligopeptides - immunology ; synthetic peptides</subject><ispartof>Journal of immunological methods, 1985-04, Vol.78 (1), p.59-69</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-30bc35e81a0b6a908e715d94006fa31c8b9373277c1f15d87bf2a96a8e7b9dfa3</citedby><cites>FETCH-LOGICAL-c452t-30bc35e81a0b6a908e715d94006fa31c8b9373277c1f15d87bf2a96a8e7b9dfa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(85)90329-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8399193$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2580027$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Briand, J.P.</creatorcontrib><creatorcontrib>Muller, S.</creatorcontrib><creatorcontrib>Van Regenmortel, M.H.V.</creatorcontrib><title>Synthetic peptides as antigens: Pitfalls of conjugation methods</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>Peptide-carrier conjugates were prepared using 9 different synthetic peptides, 3 carrier proteins and 4 coupling reagents. Residues of the carrier protein that were modified by different coupling reagents (e.g., glutaraldehyde, carbodiimides, bis-diazotized benzidine) were found to elicit specific antibodies that reacted with unrelated carrier proteins treated with the same coupling agent. To demonstrate the presence of peptide antibodies in an antiserum raised against a peptide-carrier conjugate, it was necessary to use as antigen the peptide coupled to another carrier by means of a different coupling agent.
Some of the commonly used conjugation methods were found to lead to conjugates of insufficient stability and sometimes also altered the antigenic properties of the peptide moiety. These difficulties can be overcome by additional control experiments designed to test the quality and the peptide-carrier conjugates.</description><subject>Antigenic determinants, haptens, artificial antigens</subject><subject>Antigens</subject><subject>Biological and medical sciences</subject><subject>bis-diazotized benzidine</subject><subject>carbodiimides</subject><subject>Carrier Proteins - immunology</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>coupling procedures</subject><subject>Epitopes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>glutaraldehyde</subject><subject>Immunosorbent Techniques</subject><subject>Molecular immunology</subject><subject>Oligopeptides - chemical synthesis</subject><subject>Oligopeptides - immunology</subject><subject>synthetic peptides</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQQIMotVb_gcIeRPSwOtl0N4kHRYpfUFBQzyGbnW1T9qNuUqH_3tQuPQqBObw3Q3iEnFK4pkCzG4AkiSlP5aVIrySwRMZ0jwyp4EnMJaT7ZLhTDsmRcwsAoJDBgAySVATGh-T-Y934OXproiUuvS3QRTq8xtsZNu42ere-1FXloraMTNssVjPtbdtENfp5W7hjchCww5N-jsjX0-Pn5CWevj2_Th6msRmniY8Z5IalKKiGPNMSBHKaFnIMkJWaUSNyyThLODe0DEDwvEy0zHTwclkEZUQutneXXfu9QudVbZ3BqtINtiuneAYMBKNBHG9F07XOdViqZWdr3a0VBbXppjZR1CaKEqn666Y2a2f9_VVeY7Fb6kMFft5z7Yyuyk43xrqdJpiUVLKg3W01DC1-LHbKGYuNwcJ2aLwqWvv_P34BUqiIfA</recordid><startdate>19850408</startdate><enddate>19850408</enddate><creator>Briand, J.P.</creator><creator>Muller, S.</creator><creator>Van Regenmortel, M.H.V.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850408</creationdate><title>Synthetic peptides as antigens: Pitfalls of conjugation methods</title><author>Briand, J.P. ; Muller, S. ; Van Regenmortel, M.H.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-30bc35e81a0b6a908e715d94006fa31c8b9373277c1f15d87bf2a96a8e7b9dfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Antigenic determinants, haptens, artificial antigens</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>bis-diazotized benzidine</topic><topic>carbodiimides</topic><topic>Carrier Proteins - immunology</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>coupling procedures</topic><topic>Epitopes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>glutaraldehyde</topic><topic>Immunosorbent Techniques</topic><topic>Molecular immunology</topic><topic>Oligopeptides - chemical synthesis</topic><topic>Oligopeptides - immunology</topic><topic>synthetic peptides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Briand, J.P.</creatorcontrib><creatorcontrib>Muller, S.</creatorcontrib><creatorcontrib>Van Regenmortel, M.H.V.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Briand, J.P.</au><au>Muller, S.</au><au>Van Regenmortel, M.H.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthetic peptides as antigens: Pitfalls of conjugation methods</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1985-04-08</date><risdate>1985</risdate><volume>78</volume><issue>1</issue><spage>59</spage><epage>69</epage><pages>59-69</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>Peptide-carrier conjugates were prepared using 9 different synthetic peptides, 3 carrier proteins and 4 coupling reagents. Residues of the carrier protein that were modified by different coupling reagents (e.g., glutaraldehyde, carbodiimides, bis-diazotized benzidine) were found to elicit specific antibodies that reacted with unrelated carrier proteins treated with the same coupling agent. To demonstrate the presence of peptide antibodies in an antiserum raised against a peptide-carrier conjugate, it was necessary to use as antigen the peptide coupled to another carrier by means of a different coupling agent.
Some of the commonly used conjugation methods were found to lead to conjugates of insufficient stability and sometimes also altered the antigenic properties of the peptide moiety. These difficulties can be overcome by additional control experiments designed to test the quality and the peptide-carrier conjugates.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2580027</pmid><doi>10.1016/0022-1759(85)90329-1</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1985-04, Vol.78 (1), p.59-69 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Antigenic determinants, haptens, artificial antigens Antigens Biological and medical sciences bis-diazotized benzidine carbodiimides Carrier Proteins - immunology Chemical Phenomena Chemistry coupling procedures Epitopes Fundamental and applied biological sciences. Psychology Fundamental immunology glutaraldehyde Immunosorbent Techniques Molecular immunology Oligopeptides - chemical synthesis Oligopeptides - immunology synthetic peptides |
| title | Synthetic peptides as antigens: Pitfalls of conjugation methods |
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