Targeting of recombinant Na +/glucose cotransporter (SGLT1) to the apical membrane

A full-length Na +/glucose cotransporter cDNA (SGLT1) from rabbit intestine was subcloned into the pMAMneo mammalian expression vector and transfected by Ca 2+ precipitation into Madin-Darby canine kidney (MDCK) cells. Stable MDCK transfectants isolated after clonal isolation and selection in G418 e...

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Veröffentlicht in:	FEBS letters 1993-10, Vol.333 (1), p.1-4
Hauptverfasser:	Kong, Cheng-Te, Varde, Anjali, Lever, Julia E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Binding and carrier proteins Biological and medical sciences Biological Transport Blotting, Northern Blotting, Western Cell Line dex, dexamethasone Dogs Fundamental and applied biological sciences. Psychology Madin-Darby canine kidney cell MDCK, Madin-Darby canine kidney Membrane polarity Membrane Proteins - metabolism Monosaccharide Transport Proteins - metabolism Proteins Rabbits Recombinant Proteins - metabolism Sodium/glucose cotransport Transfection αMGP, α-D-methylglucopyranoside
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