Targeting of recombinant Na +/glucose cotransporter (SGLT1) to the apical membrane
A full-length Na +/glucose cotransporter cDNA (SGLT1) from rabbit intestine was subcloned into the pMAMneo mammalian expression vector and transfected by Ca 2+ precipitation into Madin-Darby canine kidney (MDCK) cells. Stable MDCK transfectants isolated after clonal isolation and selection in G418 e...
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| Veröffentlicht in: | FEBS letters 1993-10, Vol.333 (1), p.1-4 |
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| creator | Kong, Cheng-Te Varde, Anjali Lever, Julia E. |
| description | A full-length Na
+/glucose cotransporter cDNA (SGLT1) from rabbit intestine was subcloned into the pMAMneo mammalian expression vector and transfected by Ca
2+ precipitation into Madin-Darby canine kidney (MDCK) cells. Stable MDCK transfectants isolated after clonal isolation and selection in G418 exhibited dexamethasone-inducible Na
+/glucose cotransport activity under regulation of the MMTV promoter of the vector. Transfectants expressed the recombinant 75 kDa Na
+/glucose cotransporter subunit as shown by Western blot, and SGLT1 mRNA as shown by Northern blot, but these were undetectable in untransfected MDCK cells. Over 93% of total recombinant transport activity was targeted to the apical membrane. This indicates that the primary amino acid sequence of SGLT1 contains the information necessary to target this transporter to the apical membrane. |
| doi_str_mv | 10.1016/0014-5793(93)80363-Y |
| format | Article |
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+/glucose cotransporter cDNA (SGLT1) from rabbit intestine was subcloned into the pMAMneo mammalian expression vector and transfected by Ca
2+ precipitation into Madin-Darby canine kidney (MDCK) cells. Stable MDCK transfectants isolated after clonal isolation and selection in G418 exhibited dexamethasone-inducible Na
+/glucose cotransport activity under regulation of the MMTV promoter of the vector. Transfectants expressed the recombinant 75 kDa Na
+/glucose cotransporter subunit as shown by Western blot, and SGLT1 mRNA as shown by Northern blot, but these were undetectable in untransfected MDCK cells. Over 93% of total recombinant transport activity was targeted to the apical membrane. This indicates that the primary amino acid sequence of SGLT1 contains the information necessary to target this transporter to the apical membrane.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(93)80363-Y</identifier><identifier>PMID: 8224142</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Binding and carrier proteins ; Biological and medical sciences ; Biological Transport ; Blotting, Northern ; Blotting, Western ; Cell Line ; dex, dexamethasone ; Dogs ; Fundamental and applied biological sciences. Psychology ; Madin-Darby canine kidney cell ; MDCK, Madin-Darby canine kidney ; Membrane polarity ; Membrane Proteins - metabolism ; Monosaccharide Transport Proteins - metabolism ; Proteins ; Rabbits ; Recombinant Proteins - metabolism ; Sodium/glucose cotransport ; Transfection ; αMGP, α-D-methylglucopyranoside</subject><ispartof>FEBS letters, 1993-10, Vol.333 (1), p.1-4</ispartof><rights>1993</rights><rights>FEBS Letters 333 (1993) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495Y-bbc880edcdd1256a7ebe720dc337f7c7eb7e1f436c55a2fe2650a1f0d5e8bf7b3</citedby><cites>FETCH-LOGICAL-c495Y-bbc880edcdd1256a7ebe720dc337f7c7eb7e1f436c55a2fe2650a1f0d5e8bf7b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(93)80363-Y$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3910726$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8224142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kong, Cheng-Te</creatorcontrib><creatorcontrib>Varde, Anjali</creatorcontrib><creatorcontrib>Lever, Julia E.</creatorcontrib><title>Targeting of recombinant Na +/glucose cotransporter (SGLT1) to the apical membrane</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A full-length Na
+/glucose cotransporter cDNA (SGLT1) from rabbit intestine was subcloned into the pMAMneo mammalian expression vector and transfected by Ca
2+ precipitation into Madin-Darby canine kidney (MDCK) cells. Stable MDCK transfectants isolated after clonal isolation and selection in G418 exhibited dexamethasone-inducible Na
+/glucose cotransport activity under regulation of the MMTV promoter of the vector. Transfectants expressed the recombinant 75 kDa Na
+/glucose cotransporter subunit as shown by Western blot, and SGLT1 mRNA as shown by Northern blot, but these were undetectable in untransfected MDCK cells. Over 93% of total recombinant transport activity was targeted to the apical membrane. This indicates that the primary amino acid sequence of SGLT1 contains the information necessary to target this transporter to the apical membrane.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Binding and carrier proteins</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>dex, dexamethasone</subject><subject>Dogs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Madin-Darby canine kidney cell</subject><subject>MDCK, Madin-Darby canine kidney</subject><subject>Membrane polarity</subject><subject>Membrane Proteins - metabolism</subject><subject>Monosaccharide Transport Proteins - metabolism</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sodium/glucose cotransport</subject><subject>Transfection</subject><subject>αMGP, α-D-methylglucopyranoside</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1L5DAUhoMoOn78A4VciIxINR9t094IqziuMLiwjhdzFdL0ZIy0zZh0dvHfb-oMcykLgXDOec6bc94gdErJNSU0vyGEpkkmSj4u-WVBeM6T-Q4a0ULwhKd5sYtGW-QAHYbwTmJc0HIf7ReMpTRlI_R7pvwCetstsDPYg3ZtZTvV9fhZ4aubRbPSLgDWrveqC0vne_B4_PI4ndFL3DvcvwFWS6tVg1toqwjBMdozqglwsrmP0OvkYXb_M5n-eny6_zFNdFpm86SqdFEQqHVdU5blSkAFgpFacy6M0DEUQE3Kc51lihlgeUYUNaTOoKiMqPgRuljrLr37WEHoZWuDhqaJM7hVkCInnGQpi2C6BrV3IXgwcultq_ynpEQOVsrBJzn4JOP5slLOY9vZRn9VtVBvmzbexfr5pq5C3N_E3bUNW4yXlAiWR2yyxv7aBj7_62k5ebhjQ2HIl_wrO8xzuxaCaOofC14GbaHTUNv4b72snf1-oX9sIaMv</recordid><startdate>19931025</startdate><enddate>19931025</enddate><creator>Kong, Cheng-Te</creator><creator>Varde, Anjali</creator><creator>Lever, Julia E.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931025</creationdate><title>Targeting of recombinant Na +/glucose cotransporter (SGLT1) to the apical membrane</title><author>Kong, Cheng-Te ; Varde, Anjali ; Lever, Julia E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495Y-bbc880edcdd1256a7ebe720dc337f7c7eb7e1f436c55a2fe2650a1f0d5e8bf7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Binding and carrier proteins</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Blotting, Northern</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>dex, dexamethasone</topic><topic>Dogs</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Madin-Darby canine kidney cell</topic><topic>MDCK, Madin-Darby canine kidney</topic><topic>Membrane polarity</topic><topic>Membrane Proteins - metabolism</topic><topic>Monosaccharide Transport Proteins - metabolism</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sodium/glucose cotransport</topic><topic>Transfection</topic><topic>αMGP, α-D-methylglucopyranoside</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kong, Cheng-Te</creatorcontrib><creatorcontrib>Varde, Anjali</creatorcontrib><creatorcontrib>Lever, Julia E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kong, Cheng-Te</au><au>Varde, Anjali</au><au>Lever, Julia E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeting of recombinant Na +/glucose cotransporter (SGLT1) to the apical membrane</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1993-10-25</date><risdate>1993</risdate><volume>333</volume><issue>1</issue><spage>1</spage><epage>4</epage><pages>1-4</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>A full-length Na
+/glucose cotransporter cDNA (SGLT1) from rabbit intestine was subcloned into the pMAMneo mammalian expression vector and transfected by Ca
2+ precipitation into Madin-Darby canine kidney (MDCK) cells. Stable MDCK transfectants isolated after clonal isolation and selection in G418 exhibited dexamethasone-inducible Na
+/glucose cotransport activity under regulation of the MMTV promoter of the vector. Transfectants expressed the recombinant 75 kDa Na
+/glucose cotransporter subunit as shown by Western blot, and SGLT1 mRNA as shown by Northern blot, but these were undetectable in untransfected MDCK cells. Over 93% of total recombinant transport activity was targeted to the apical membrane. This indicates that the primary amino acid sequence of SGLT1 contains the information necessary to target this transporter to the apical membrane.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8224142</pmid><doi>10.1016/0014-5793(93)80363-Y</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1993-10, Vol.333 (1), p.1-4 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Animals Binding and carrier proteins Biological and medical sciences Biological Transport Blotting, Northern Blotting, Western Cell Line dex, dexamethasone Dogs Fundamental and applied biological sciences. Psychology Madin-Darby canine kidney cell MDCK, Madin-Darby canine kidney Membrane polarity Membrane Proteins - metabolism Monosaccharide Transport Proteins - metabolism Proteins Rabbits Recombinant Proteins - metabolism Sodium/glucose cotransport Transfection αMGP, α-D-methylglucopyranoside |
| title | Targeting of recombinant Na +/glucose cotransporter (SGLT1) to the apical membrane |
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