Structural studies of Escherichia coli O127 O-antigen polysaccharide
The O-specific side-chain of the lipopolysaccharide from Escherichia coli O127a;4932-53) has been investigated using 2D NMR spectroscopy, methylation analysis, and partial solvolysis with anhydrous hydrogen flouride as the principal methods. It is concluded that the polysaccharide is composed of tet...
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| Veröffentlicht in: | Carbohydrate research 1993-09, Vol.247, p.255-262 |
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| creator | Widmalm, Göran Leontein, Karin |
| description | The O-specific side-chain of the lipopolysaccharide from
Escherichia coli O127a;4932-53) has been investigated using 2D NMR spectroscopy, methylation analysis, and partial solvolysis with anhydrous hydrogen flouride as the principal methods. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-unites having the following structure. →)-α-
l-Fuc
p-(1 → 2)-β-
d-Gal
p-(1 → 3)-α-
d-gal
pNAc-(1 → 3)-α-
d-Gal
pNAc-(1 →
The polysaccharide contains approximately one mole of
O-acetyl groups per repeating unit distributed over several positons. |
| doi_str_mv | 10.1016/0008-6215(93)84258-8 |
| format | Article |
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Escherichia coli O127a;4932-53) has been investigated using 2D NMR spectroscopy, methylation analysis, and partial solvolysis with anhydrous hydrogen flouride as the principal methods. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-unites having the following structure. →)-α-
l-Fuc
p-(1 → 2)-β-
d-Gal
p-(1 → 3)-α-
d-gal
pNAc-(1 → 3)-α-
d-Gal
pNAc-(1 →
The polysaccharide contains approximately one mole of
O-acetyl groups per repeating unit distributed over several positons.</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>DOI: 10.1016/0008-6215(93)84258-8</identifier><identifier>PMID: 7693348</identifier><identifier>CODEN: CRBRAT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Bacteriology ; Biological and medical sciences ; Carbohydrate Conformation ; Carbohydrate Sequence ; Escherichia coli - chemistry ; Escherichia coli - immunology ; Fundamental and applied biological sciences. Psychology ; Indicators and Reagents ; Lipopolysaccharides - chemistry ; Lipopolysaccharides - isolation & purification ; Magnetic Resonance Spectroscopy - methods ; Methylation ; Microbiology ; Models, Molecular ; Molecular Sequence Data ; Morphology, structure, chemical composition ; O Antigens ; Oligosaccharides - chemistry ; Oligosaccharides - isolation & purification ; Polysaccharides, Bacterial - chemistry ; Polysaccharides, Bacterial - isolation & purification</subject><ispartof>Carbohydrate research, 1993-09, Vol.247, p.255-262</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-986db8ccef8cbfd1d8c794fd55e9e6c78af34bba9e9db11daf4271d00647347f3</citedby><cites>FETCH-LOGICAL-c386t-986db8ccef8cbfd1d8c794fd55e9e6c78af34bba9e9db11daf4271d00647347f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0008-6215(93)84258-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4907568$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7693348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Widmalm, Göran</creatorcontrib><creatorcontrib>Leontein, Karin</creatorcontrib><title>Structural studies of Escherichia coli O127 O-antigen polysaccharide</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>The O-specific side-chain of the lipopolysaccharide from
Escherichia coli O127a;4932-53) has been investigated using 2D NMR spectroscopy, methylation analysis, and partial solvolysis with anhydrous hydrogen flouride as the principal methods. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-unites having the following structure. →)-α-
l-Fuc
p-(1 → 2)-β-
d-Gal
p-(1 → 3)-α-
d-gal
pNAc-(1 → 3)-α-
d-Gal
pNAc-(1 →
The polysaccharide contains approximately one mole of
O-acetyl groups per repeating unit distributed over several positons.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Indicators and Reagents</subject><subject>Lipopolysaccharides - chemistry</subject><subject>Lipopolysaccharides - isolation & purification</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Methylation</subject><subject>Microbiology</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Morphology, structure, chemical composition</subject><subject>O Antigens</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - isolation & purification</subject><subject>Polysaccharides, Bacterial - chemistry</subject><subject>Polysaccharides, Bacterial - isolation & purification</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LJDEQhsOyoqPuP1ihD8uih9akk87HRVj8BmEOurC3kK5UdiI93WPSLfjv7XGGOXoqivep4uUh5Cej54wyeUEp1aWsWH1q-JkWVa1L_Y3MmFa8FJX8953MdsgBOcz5ZVqpVHKf7CtpOBd6Rq6fhjTCMCbXFnkYfcRc9KG4ybDAFGERXQF9G4s5q1QxL103xP_YFau-fc8OYOFS9HhM9oJrM_7YziPy9_bm-eq-fJzfPVz9eSyBazmURkvfaAAMGprgmdegjAi-rtGgBKVd4KJpnEHjG8a8C6JSzE-dheJCBX5Efm_-rlL_OmIe7DJmwLZ1HfZjtkrSimtGJ1BsQEh9zgmDXaW4dOndMmrX8uzajF2bsYbbT3lWT2cn2_9js0S_O9ramvJf29xlcG1IroOYd5gwVNVyjV1uMJxcvEVMNkPEDtDHhDBY38eve3wAPGWLFA</recordid><startdate>19930902</startdate><enddate>19930902</enddate><creator>Widmalm, Göran</creator><creator>Leontein, Karin</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930902</creationdate><title>Structural studies of Escherichia coli O127 O-antigen polysaccharide</title><author>Widmalm, Göran ; Leontein, Karin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-986db8ccef8cbfd1d8c794fd55e9e6c78af34bba9e9db11daf4271d00647347f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Indicators and Reagents</topic><topic>Lipopolysaccharides - chemistry</topic><topic>Lipopolysaccharides - isolation & purification</topic><topic>Magnetic Resonance Spectroscopy - methods</topic><topic>Methylation</topic><topic>Microbiology</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Morphology, structure, chemical composition</topic><topic>O Antigens</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - isolation & purification</topic><topic>Polysaccharides, Bacterial - chemistry</topic><topic>Polysaccharides, Bacterial - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Widmalm, Göran</creatorcontrib><creatorcontrib>Leontein, Karin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Widmalm, Göran</au><au>Leontein, Karin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural studies of Escherichia coli O127 O-antigen polysaccharide</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>1993-09-02</date><risdate>1993</risdate><volume>247</volume><spage>255</spage><epage>262</epage><pages>255-262</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><coden>CRBRAT</coden><abstract>The O-specific side-chain of the lipopolysaccharide from
Escherichia coli O127a;4932-53) has been investigated using 2D NMR spectroscopy, methylation analysis, and partial solvolysis with anhydrous hydrogen flouride as the principal methods. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-unites having the following structure. →)-α-
l-Fuc
p-(1 → 2)-β-
d-Gal
p-(1 → 3)-α-
d-gal
pNAc-(1 → 3)-α-
d-Gal
pNAc-(1 →
The polysaccharide contains approximately one mole of
O-acetyl groups per repeating unit distributed over several positons.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>7693348</pmid><doi>10.1016/0008-6215(93)84258-8</doi><tpages>8</tpages></addata></record> |
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| ispartof | Carbohydrate research, 1993-09, Vol.247, p.255-262 |
| issn | 0008-6215 1873-426X |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacteriology Biological and medical sciences Carbohydrate Conformation Carbohydrate Sequence Escherichia coli - chemistry Escherichia coli - immunology Fundamental and applied biological sciences. Psychology Indicators and Reagents Lipopolysaccharides - chemistry Lipopolysaccharides - isolation & purification Magnetic Resonance Spectroscopy - methods Methylation Microbiology Models, Molecular Molecular Sequence Data Morphology, structure, chemical composition O Antigens Oligosaccharides - chemistry Oligosaccharides - isolation & purification Polysaccharides, Bacterial - chemistry Polysaccharides, Bacterial - isolation & purification |
| title | Structural studies of Escherichia coli O127 O-antigen polysaccharide |
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