Cloning and expression of the cDNA coding for aequorin, a bioluminescent calcium-binding protein

Aequorin is a bioluminescent protein which consists of a polypeptide chain (apoaequorin), coelenterate luciferin, and bound oxygen. Aequorin produces blue light upon binding Ca 2+. We have isolated six recombinant pBR322 plasmids which contain apoaequorin cDNA sequences. A mixed synthetic oligonucle...

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Veröffentlicht in:	Biochemical and biophysical research communications 1985-02, Vol.126 (3), p.1259-1268
Hauptverfasser:	Prasher, Douglas, McCann, Richard O., Cormier, Milton J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aequorea victoria Aequorin - genetics Base Sequence Cloning, Molecular DNA Escherichia coli - genetics Gene Expression Regulation Luminescent Proteins - genetics Peptides - isolation & purification Poly A - metabolism Protein Biosynthesis RNA - metabolism RNA, Messenger
Online-Zugang:	Volltext
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