Direct correlation between genome dimerization and recombination efficiency of HIV-1
More than ten subtypes of Human immunodeficiency virus type 1 (HIV-1) have been identified, and many inter-subtype recombinant viruses have been isolated. The genome of HIV-1 is a single-stranded positive sense RNA, and is always found as dimers in virus particles. Frequent recombination between two...
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| Veröffentlicht in: | Microbes and infection 2010-11, Vol.12 (12-13), p.1002-1011 |
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| creator | Sakuragi, Jun-ichi Sakuragi, Sayuri Ohishi, Masahisa Shioda, Tatsuo |
| description | More than ten subtypes of Human immunodeficiency virus type 1 (HIV-1) have been identified, and many inter-subtype recombinant viruses have been isolated. The genome of HIV-1 is a single-stranded positive sense RNA, and is always found as dimers in virus particles. Frequent recombination between two genomes during reverse transcription is often observed and thus reasonable to assume that genome dimerization controls viral genomic recombination. Recently, several reports indicated in vitro/in vivo data to support this idea. In the study reported here, in an attempt to show a comprehensive evidence, we compared the efficiency of various inter-subtype dimerization and recombination and detected a near-complete correlation of the two functions. This suggests that genome dimerization controls recombination and plays an important role in promoting the genetic diversity of HIV-1 in general. We also investigated various inter-subtype hetero-dimerization within HIV-1 virions, and found that the dimer initiation site is a major, but not the sole determinant of dimerization (and recombination) efficiency. |
| doi_str_mv | 10.1016/j.micinf.2010.06.012 |
| format | Article |
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The genome of HIV-1 is a single-stranded positive sense RNA, and is always found as dimers in virus particles. Frequent recombination between two genomes during reverse transcription is often observed and thus reasonable to assume that genome dimerization controls viral genomic recombination. Recently, several reports indicated in vitro/in vivo data to support this idea. In the study reported here, in an attempt to show a comprehensive evidence, we compared the efficiency of various inter-subtype dimerization and recombination and detected a near-complete correlation of the two functions. This suggests that genome dimerization controls recombination and plays an important role in promoting the genetic diversity of HIV-1 in general. 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The genome of HIV-1 is a single-stranded positive sense RNA, and is always found as dimers in virus particles. Frequent recombination between two genomes during reverse transcription is often observed and thus reasonable to assume that genome dimerization controls viral genomic recombination. Recently, several reports indicated in vitro/in vivo data to support this idea. In the study reported here, in an attempt to show a comprehensive evidence, we compared the efficiency of various inter-subtype dimerization and recombination and detected a near-complete correlation of the two functions. This suggests that genome dimerization controls recombination and plays an important role in promoting the genetic diversity of HIV-1 in general. 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Psychology</subject><subject>Genetic diversity</subject><subject>Genome, Viral</subject><subject>Genomes</subject><subject>genomics</subject><subject>HIV</subject><subject>HIV-1 - genetics</subject><subject>Human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Packaging</subject><subject>Recombination</subject><subject>Recombination, Genetic</subject><subject>Reverse transcription</subject><subject>RNA</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - metabolism</subject><subject>Sequence Alignment</subject><subject>Virions</subject><subject>Virology</subject><issn>1286-4579</issn><issn>1769-714X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v3CAQhlGVqEnT_oOq8iVqL94MGGNziVSlzYcUKZe06g1hGCpWNiTgbZX8-rLxprnlBBqedxgeCPlIYUWBipP1avLGB7diUEogVkDZG3JIOyHrjvJfe2XPelHztpMH5F3OawDadoK_JQcMRNP1kh6S228-oZkrE1PCUc8-hmrA-S9iqH5jiBNW1k-Y_ONypoOtSiBOgw9LBZ0rc2AwD1V01eXVz5q-J_tOjxk_7NYj8uP8--3ZZX19c3F19vW6NlzCXHPZGSe4to1GY2TPjQXmkFuBDZUDd8gkxaGxZmDcAWLnuJTlfWwAoxGbI_J56XuX4v0G86wmnw2Oow4YN1l1AljTCioL-eVVkgKDvm1bwQvKF9SkmHNCp-6Sn3R6KJDamldrtZhXW_MKhCrmS-zT7obNMKH9H3pWXYDjHaCz0aNLOhifX7imkbyHbaPThcNi7o_HpPKTXrRPP6Vs9K9P8g_NvKQ-</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Sakuragi, Jun-ichi</creator><creator>Sakuragi, Sayuri</creator><creator>Ohishi, Masahisa</creator><creator>Shioda, Tatsuo</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20101101</creationdate><title>Direct correlation between genome dimerization and recombination efficiency of HIV-1</title><author>Sakuragi, Jun-ichi ; Sakuragi, Sayuri ; Ohishi, Masahisa ; Shioda, Tatsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-497cf64ad3aecc984cd02fe4d6e319b4fe291eb3dcb24f0ee7f4997692b0caee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Data processing</topic><topic>Dimerization</topic><topic>Fundamental and applied biological sciences. 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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Base Sequence Biological and medical sciences Data processing Dimerization Fundamental and applied biological sciences. Psychology Genetic diversity Genome, Viral Genomes genomics HIV HIV-1 - genetics Human immunodeficiency virus 1 Humans Microbiology Miscellaneous Molecular Sequence Data Packaging Recombination Recombination, Genetic Reverse transcription RNA RNA, Viral - genetics RNA, Viral - metabolism Sequence Alignment Virions Virology |
| title | Direct correlation between genome dimerization and recombination efficiency of HIV-1 |
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