p53 -Dependent Adaptive Responses in Human Cells Exposed to Space Radiations
It has been reported that priming irradiation or conditioning irradiation with a low dose of X-rays in the range of 0.02–0.1 Gy induces a p53-dependent adaptive response in mammalian cells. The aim of the present study was to clarify the effect of space radiations on the adaptive response. Two human...
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| Veröffentlicht in: | International journal of radiation oncology, biology, physics biology, physics, 2010-11, Vol.78 (4), p.1171-1176 |
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| container_title | International journal of radiation oncology, biology, physics |
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| creator | Takahashi, Akihisa Su, Xiaoming Suzuki, Hiromi Omori, Katsunori Seki, Masaya Hashizume, Toko Shimazu, Toru Ishioka, Noriaki Iwasaki, Toshiyasu Ohnishi, Takeo |
| description | It has been reported that priming irradiation or conditioning irradiation with a low dose of X-rays in the range of 0.02–0.1 Gy induces a
p53-dependent adaptive response in mammalian cells. The aim of the present study was to clarify the effect of space radiations on the adaptive response.
Two human lymphoblastoid cell lines were used; one cell line bears a wild-type
p53 (wt
p53) gene, and another cell line bears a mutated
p53 (m
p53) gene. The cells were frozen during transportation on the space shuttle and while in orbit in the International Space Station freezer for 133 days between November 15, 2008 and March 29, 2009. After the frozen samples were returned to Earth, the cells were cultured for 6 h and then exposed to a challenging X-ray-irradiation (2 Gy). Cellular sensitivity, apoptosis, and chromosome aberrations were scored using dye-exclusion assays, Hoechst33342 staining assays, and chromosomal banding techniques, respectively.
In cells exposed to space radiations, adaptive responses such as the induction of radioresistance and the depression of radiation-induced apoptosis and chromosome aberrations were observed in wt
p53 cells but not in m
p53 cells.
These results have confirmed the hypothesis that
p53-dependent adaptive responses are apparently induced by space radiations within a specific range of low doses. The cells exhibited this effect owing to space radiations exposure, even though the doses in space were very low. |
| doi_str_mv | 10.1016/j.ijrobp.2010.04.062 |
| format | Article |
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p53-dependent adaptive response in mammalian cells. The aim of the present study was to clarify the effect of space radiations on the adaptive response.
Two human lymphoblastoid cell lines were used; one cell line bears a wild-type
p53 (wt
p53) gene, and another cell line bears a mutated
p53 (m
p53) gene. The cells were frozen during transportation on the space shuttle and while in orbit in the International Space Station freezer for 133 days between November 15, 2008 and March 29, 2009. After the frozen samples were returned to Earth, the cells were cultured for 6 h and then exposed to a challenging X-ray-irradiation (2 Gy). Cellular sensitivity, apoptosis, and chromosome aberrations were scored using dye-exclusion assays, Hoechst33342 staining assays, and chromosomal banding techniques, respectively.
In cells exposed to space radiations, adaptive responses such as the induction of radioresistance and the depression of radiation-induced apoptosis and chromosome aberrations were observed in wt
p53 cells but not in m
p53 cells.
These results have confirmed the hypothesis that
p53-dependent adaptive responses are apparently induced by space radiations within a specific range of low doses. The cells exhibited this effect owing to space radiations exposure, even though the doses in space were very low.</description><identifier>ISSN: 0360-3016</identifier><identifier>EISSN: 1879-355X</identifier><identifier>DOI: 10.1016/j.ijrobp.2010.04.062</identifier><identifier>PMID: 20729005</identifier><identifier>CODEN: IOBPD3</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adaptation, Physiological - physiology ; Adaptation, Physiological - radiation effects ; Adaptive response ; Apoptosis - physiology ; Apoptosis - radiation effects ; Biological and medical sciences ; Biological effects of radiation ; Cell Count ; Cell Line ; Chromosome Aberrations - radiation effects ; Cosmic Radiation ; Cryopreservation ; Fundamental and applied biological sciences. Psychology ; Genes, p53 - physiology ; Genes, p53 - radiation effects ; Humans ; Ionizing radiations ; Lymphocytes - physiology ; Lymphocytes - radiation effects ; p53 ; Radiation Dosage ; Radiation Tolerance - physiology ; Radiation Tolerance - radiation effects ; Space Flight ; Space radiations ; Spaceflight ; Tissues, organs and organisms biophysics</subject><ispartof>International journal of radiation oncology, biology, physics, 2010-11, Vol.78 (4), p.1171-1176</ispartof><rights>2010 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijrobp.2010.04.062$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23387488$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20729005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takahashi, Akihisa</creatorcontrib><creatorcontrib>Su, Xiaoming</creatorcontrib><creatorcontrib>Suzuki, Hiromi</creatorcontrib><creatorcontrib>Omori, Katsunori</creatorcontrib><creatorcontrib>Seki, Masaya</creatorcontrib><creatorcontrib>Hashizume, Toko</creatorcontrib><creatorcontrib>Shimazu, Toru</creatorcontrib><creatorcontrib>Ishioka, Noriaki</creatorcontrib><creatorcontrib>Iwasaki, Toshiyasu</creatorcontrib><creatorcontrib>Ohnishi, Takeo</creatorcontrib><title>p53 -Dependent Adaptive Responses in Human Cells Exposed to Space Radiations</title><title>International journal of radiation oncology, biology, physics</title><addtitle>Int J Radiat Oncol Biol Phys</addtitle><description>It has been reported that priming irradiation or conditioning irradiation with a low dose of X-rays in the range of 0.02–0.1 Gy induces a
p53-dependent adaptive response in mammalian cells. The aim of the present study was to clarify the effect of space radiations on the adaptive response.
Two human lymphoblastoid cell lines were used; one cell line bears a wild-type
p53 (wt
p53) gene, and another cell line bears a mutated
p53 (m
p53) gene. The cells were frozen during transportation on the space shuttle and while in orbit in the International Space Station freezer for 133 days between November 15, 2008 and March 29, 2009. After the frozen samples were returned to Earth, the cells were cultured for 6 h and then exposed to a challenging X-ray-irradiation (2 Gy). Cellular sensitivity, apoptosis, and chromosome aberrations were scored using dye-exclusion assays, Hoechst33342 staining assays, and chromosomal banding techniques, respectively.
In cells exposed to space radiations, adaptive responses such as the induction of radioresistance and the depression of radiation-induced apoptosis and chromosome aberrations were observed in wt
p53 cells but not in m
p53 cells.
These results have confirmed the hypothesis that
p53-dependent adaptive responses are apparently induced by space radiations within a specific range of low doses. The cells exhibited this effect owing to space radiations exposure, even though the doses in space were very low.</description><subject>Adaptation, Physiological - physiology</subject><subject>Adaptation, Physiological - radiation effects</subject><subject>Adaptive response</subject><subject>Apoptosis - physiology</subject><subject>Apoptosis - radiation effects</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Cell Count</subject><subject>Cell Line</subject><subject>Chromosome Aberrations - radiation effects</subject><subject>Cosmic Radiation</subject><subject>Cryopreservation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, p53 - physiology</subject><subject>Genes, p53 - radiation effects</subject><subject>Humans</subject><subject>Ionizing radiations</subject><subject>Lymphocytes - physiology</subject><subject>Lymphocytes - radiation effects</subject><subject>p53</subject><subject>Radiation Dosage</subject><subject>Radiation Tolerance - physiology</subject><subject>Radiation Tolerance - radiation effects</subject><subject>Space Flight</subject><subject>Space radiations</subject><subject>Spaceflight</subject><subject>Tissues, organs and organisms biophysics</subject><issn>0360-3016</issn><issn>1879-355X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0U1LAzEQBuAgiq3VfyCSi3jaOvnYzfYiSK1WKAh-gLeQTWYhpd2Nm23Rf29KK54GhodhZl5CLhmMGbDidjn2y66twphDaoEcQ8GPyJCVapKJPP88JkMQBWQi4QE5i3EJAIwpeUoGHBSfAORDsgi5oNkDBmwcNj29dyb0fov0FWNom4iR-obON2vT0CmuVpHOvkMb0dG-pW_B2CSN86b3CZ-Tk9qsIl4c6oh8PM7ep_Ns8fL0PL1fZCjEpM-EUwVYxVwOk1KaShmppKytsuAqCzUvauFkWTOey1KB5GAEIre5K6tcmUKMyM1-bujarw3GXq99tGk702C7iTqN50LyQiZ5dZCbao1Oh86vTfej_x6QwPUBmGjNqu5MY338d0KUSpZlcnd7h-murcdOR-uxseh8h7bXrvWagd4lo5d6n4zeJaNB6pSM-AXf3n-c</recordid><startdate>20101115</startdate><enddate>20101115</enddate><creator>Takahashi, Akihisa</creator><creator>Su, Xiaoming</creator><creator>Suzuki, Hiromi</creator><creator>Omori, Katsunori</creator><creator>Seki, Masaya</creator><creator>Hashizume, Toko</creator><creator>Shimazu, Toru</creator><creator>Ishioka, Noriaki</creator><creator>Iwasaki, Toshiyasu</creator><creator>Ohnishi, Takeo</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20101115</creationdate><title>p53 -Dependent Adaptive Responses in Human Cells Exposed to Space Radiations</title><author>Takahashi, Akihisa ; Su, Xiaoming ; Suzuki, Hiromi ; Omori, Katsunori ; Seki, Masaya ; Hashizume, Toko ; Shimazu, Toru ; Ishioka, Noriaki ; Iwasaki, Toshiyasu ; Ohnishi, Takeo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e339t-3d760c71d50984ab7a4744fc7c0dbc0f26f3d48f1254870420a3ee2c5d8b57a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adaptation, Physiological - physiology</topic><topic>Adaptation, Physiological - radiation effects</topic><topic>Adaptive response</topic><topic>Apoptosis - physiology</topic><topic>Apoptosis - radiation effects</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Cell Count</topic><topic>Cell Line</topic><topic>Chromosome Aberrations - radiation effects</topic><topic>Cosmic Radiation</topic><topic>Cryopreservation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, p53 - physiology</topic><topic>Genes, p53 - radiation effects</topic><topic>Humans</topic><topic>Ionizing radiations</topic><topic>Lymphocytes - physiology</topic><topic>Lymphocytes - radiation effects</topic><topic>p53</topic><topic>Radiation Dosage</topic><topic>Radiation Tolerance - physiology</topic><topic>Radiation Tolerance - radiation effects</topic><topic>Space Flight</topic><topic>Space radiations</topic><topic>Spaceflight</topic><topic>Tissues, organs and organisms biophysics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takahashi, Akihisa</creatorcontrib><creatorcontrib>Su, Xiaoming</creatorcontrib><creatorcontrib>Suzuki, Hiromi</creatorcontrib><creatorcontrib>Omori, Katsunori</creatorcontrib><creatorcontrib>Seki, Masaya</creatorcontrib><creatorcontrib>Hashizume, Toko</creatorcontrib><creatorcontrib>Shimazu, Toru</creatorcontrib><creatorcontrib>Ishioka, Noriaki</creatorcontrib><creatorcontrib>Iwasaki, Toshiyasu</creatorcontrib><creatorcontrib>Ohnishi, Takeo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of radiation oncology, biology, physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takahashi, Akihisa</au><au>Su, Xiaoming</au><au>Suzuki, Hiromi</au><au>Omori, Katsunori</au><au>Seki, Masaya</au><au>Hashizume, Toko</au><au>Shimazu, Toru</au><au>Ishioka, Noriaki</au><au>Iwasaki, Toshiyasu</au><au>Ohnishi, Takeo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>p53 -Dependent Adaptive Responses in Human Cells Exposed to Space Radiations</atitle><jtitle>International journal of radiation oncology, biology, physics</jtitle><addtitle>Int J Radiat Oncol Biol Phys</addtitle><date>2010-11-15</date><risdate>2010</risdate><volume>78</volume><issue>4</issue><spage>1171</spage><epage>1176</epage><pages>1171-1176</pages><issn>0360-3016</issn><eissn>1879-355X</eissn><coden>IOBPD3</coden><abstract>It has been reported that priming irradiation or conditioning irradiation with a low dose of X-rays in the range of 0.02–0.1 Gy induces a
p53-dependent adaptive response in mammalian cells. The aim of the present study was to clarify the effect of space radiations on the adaptive response.
Two human lymphoblastoid cell lines were used; one cell line bears a wild-type
p53 (wt
p53) gene, and another cell line bears a mutated
p53 (m
p53) gene. The cells were frozen during transportation on the space shuttle and while in orbit in the International Space Station freezer for 133 days between November 15, 2008 and March 29, 2009. After the frozen samples were returned to Earth, the cells were cultured for 6 h and then exposed to a challenging X-ray-irradiation (2 Gy). Cellular sensitivity, apoptosis, and chromosome aberrations were scored using dye-exclusion assays, Hoechst33342 staining assays, and chromosomal banding techniques, respectively.
In cells exposed to space radiations, adaptive responses such as the induction of radioresistance and the depression of radiation-induced apoptosis and chromosome aberrations were observed in wt
p53 cells but not in m
p53 cells.
These results have confirmed the hypothesis that
p53-dependent adaptive responses are apparently induced by space radiations within a specific range of low doses. The cells exhibited this effect owing to space radiations exposure, even though the doses in space were very low.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>20729005</pmid><doi>10.1016/j.ijrobp.2010.04.062</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0360-3016 |
| ispartof | International journal of radiation oncology, biology, physics, 2010-11, Vol.78 (4), p.1171-1176 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adaptation, Physiological - physiology Adaptation, Physiological - radiation effects Adaptive response Apoptosis - physiology Apoptosis - radiation effects Biological and medical sciences Biological effects of radiation Cell Count Cell Line Chromosome Aberrations - radiation effects Cosmic Radiation Cryopreservation Fundamental and applied biological sciences. Psychology Genes, p53 - physiology Genes, p53 - radiation effects Humans Ionizing radiations Lymphocytes - physiology Lymphocytes - radiation effects p53 Radiation Dosage Radiation Tolerance - physiology Radiation Tolerance - radiation effects Space Flight Space radiations Spaceflight Tissues, organs and organisms biophysics |
| title | p53 -Dependent Adaptive Responses in Human Cells Exposed to Space Radiations |
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