Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: cell cycle analysis and DNA-binding study
N(6)-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cance...
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| Veröffentlicht in: | DNA and cell biology 2010-11, Vol.29 (11), p.687-691 |
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| container_title | DNA and cell biology |
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| creator | Rajabi, Mehdi Signorelli, Paola Gorincioi, Elena Ghidoni, Riccardo Santaniello, Enzo |
| description | N(6)-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2 μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. A binding constant K(iPA-DNA) = 4.4 × 10(3) M(-1) was calculated and this value, together with the shift of absorbance, suggests iPA-DNA interaction at DNA surface. |
| doi_str_mv | 10.1089/dna.2010.1073 |
| format | Article |
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Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2 μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. A binding constant K(iPA-DNA) = 4.4 × 10(3) M(-1) was calculated and this value, together with the shift of absorbance, suggests iPA-DNA interaction at DNA surface.</description><identifier>EISSN: 1557-7430</identifier><identifier>DOI: 10.1089/dna.2010.1073</identifier><identifier>PMID: 20818975</identifier><language>eng</language><publisher>United States</publisher><subject>Absorption ; Apoptosis - drug effects ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Cell Cycle - drug effects ; Cell Line, Tumor ; Cell Proliferation - drug effects ; DNA - metabolism ; Humans ; Isopentenyladenosine - chemistry ; Isopentenyladenosine - metabolism ; Isopentenyladenosine - pharmacology ; Spectrum Analysis ; Stereoisomerism</subject><ispartof>DNA and cell biology, 2010-11, Vol.29 (11), p.687-691</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20818975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rajabi, Mehdi</creatorcontrib><creatorcontrib>Signorelli, Paola</creatorcontrib><creatorcontrib>Gorincioi, Elena</creatorcontrib><creatorcontrib>Ghidoni, Riccardo</creatorcontrib><creatorcontrib>Santaniello, Enzo</creatorcontrib><title>Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: cell cycle analysis and DNA-binding study</title><title>DNA and cell biology</title><addtitle>DNA Cell Biol</addtitle><description>N(6)-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2 μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. 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| ispartof | DNA and cell biology, 2010-11, Vol.29 (11), p.687-691 |
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| subjects | Absorption Apoptosis - drug effects Breast Neoplasms - genetics Breast Neoplasms - pathology Cell Cycle - drug effects Cell Line, Tumor Cell Proliferation - drug effects DNA - metabolism Humans Isopentenyladenosine - chemistry Isopentenyladenosine - metabolism Isopentenyladenosine - pharmacology Spectrum Analysis Stereoisomerism |
| title | Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: cell cycle analysis and DNA-binding study |
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