Hydroxyapatite surface solubility and effect on cell adhesion
In living organisms the biological hydroxyapatite is in constant contact with body fluids, such as blood serum and saliva. Thus, dissolution, solubility and precipitation take place as part of the interaction of this material with biological fluids in tissues. In this work we have obtained the solub...
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| Veröffentlicht in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2010-07, Vol.78 (2), p.177-184 |
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| container_title | Colloids and surfaces, B, Biointerfaces |
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| creator | Bertazzo, Sergio Zambuzzi, Willian F. Campos, Daniela D.P. Ogeda, Thais L. Ferreira, Carmen V. Bertran, Celso A. |
| description | In living organisms the biological hydroxyapatite is in constant contact with body fluids, such as blood serum and saliva. Thus, dissolution, solubility and precipitation take place as part of the interaction of this material with biological fluids in tissues. In this work we have obtained the solubility constant for the system formed from aqueous solutions in equilibrium with hydroxyapatite and thus indirectly obtained the composition of the modified hydroxyapatite surface. In order to check the effects of this equilibrium and of the modification that the surface of hydroxyapatite suffers in aqueous solutions, we cultured pre-osteoblasts onto hydroxyapatite discs before and after equilibrium. The results revealed key steps of the mechanism for the bioactivity of hydroxyapatite, which are the solubilization of hydroxyapatite and the equilibrium that is formed on the surface. These processes modify the hydroxyapatite surface, whose composition is changed to a new calcium phosphate compound with the chemical formula of CaHPO
4. A clear description of the transformations that occur on the surface of hydroxyapatite and of the interplay between these transformations and cell activity are two fundamental aspects of processes in which hydroxyapatite takes part, such as bone substitution, bone remodeling, osteoporosis and caries. |
| doi_str_mv | 10.1016/j.colsurfb.2010.02.027 |
| format | Article |
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4. A clear description of the transformations that occur on the surface of hydroxyapatite and of the interplay between these transformations and cell activity are two fundamental aspects of processes in which hydroxyapatite takes part, such as bone substitution, bone remodeling, osteoporosis and caries.</description><identifier>ISSN: 0927-7765</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2010.02.027</identifier><identifier>PMID: 20362420</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>3T3 Cells ; Algorithms ; Animals ; Aqueous solutions ; Bioactivity ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Biological ; Biomedical materials ; Bones ; Calcium phosphate ; Cell adhesion ; Cell Adhesion - drug effects ; Durapatite - chemistry ; Durapatite - pharmacology ; Hydrogen-Ion Concentration ; Hydroxyapatite ; Kinetics ; Mice ; Models, Biological ; Models, Chemical ; Solubility ; Solubility equilibrium ; Surface chemistry ; Surface modification ; Surface Properties ; Surgical implants ; Transformations</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2010-07, Vol.78 (2), p.177-184</ispartof><rights>2010 Elsevier B.V.</rights><rights>2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-ac0f6b4c094d14f1a15b9dbedb606752a410a2cc69d3ecc20f58465ce5ee9b883</citedby><cites>FETCH-LOGICAL-c432t-ac0f6b4c094d14f1a15b9dbedb606752a410a2cc69d3ecc20f58465ce5ee9b883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0927776510001153$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20362420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bertazzo, Sergio</creatorcontrib><creatorcontrib>Zambuzzi, Willian F.</creatorcontrib><creatorcontrib>Campos, Daniela D.P.</creatorcontrib><creatorcontrib>Ogeda, Thais L.</creatorcontrib><creatorcontrib>Ferreira, Carmen V.</creatorcontrib><creatorcontrib>Bertran, Celso A.</creatorcontrib><title>Hydroxyapatite surface solubility and effect on cell adhesion</title><title>Colloids and surfaces, B, Biointerfaces</title><addtitle>Colloids Surf B Biointerfaces</addtitle><description>In living organisms the biological hydroxyapatite is in constant contact with body fluids, such as blood serum and saliva. Thus, dissolution, solubility and precipitation take place as part of the interaction of this material with biological fluids in tissues. In this work we have obtained the solubility constant for the system formed from aqueous solutions in equilibrium with hydroxyapatite and thus indirectly obtained the composition of the modified hydroxyapatite surface. In order to check the effects of this equilibrium and of the modification that the surface of hydroxyapatite suffers in aqueous solutions, we cultured pre-osteoblasts onto hydroxyapatite discs before and after equilibrium. The results revealed key steps of the mechanism for the bioactivity of hydroxyapatite, which are the solubilization of hydroxyapatite and the equilibrium that is formed on the surface. These processes modify the hydroxyapatite surface, whose composition is changed to a new calcium phosphate compound with the chemical formula of CaHPO
4. A clear description of the transformations that occur on the surface of hydroxyapatite and of the interplay between these transformations and cell activity are two fundamental aspects of processes in which hydroxyapatite takes part, such as bone substitution, bone remodeling, osteoporosis and caries.</description><subject>3T3 Cells</subject><subject>Algorithms</subject><subject>Animals</subject><subject>Aqueous solutions</subject><subject>Bioactivity</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biological</subject><subject>Biomedical materials</subject><subject>Bones</subject><subject>Calcium phosphate</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - drug effects</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydroxyapatite</subject><subject>Kinetics</subject><subject>Mice</subject><subject>Models, Biological</subject><subject>Models, Chemical</subject><subject>Solubility</subject><subject>Solubility equilibrium</subject><subject>Surface chemistry</subject><subject>Surface modification</subject><subject>Surface Properties</subject><subject>Surgical implants</subject><subject>Transformations</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1r2zAUhkVpadNsfyH4br1xdvRhyb4YdIRtGQR6s10LWTpmCo6VSXZZ_n1l0uyyhQMvHJ7zwfsSsqKwpkDl5_3ahj5NsWvXDHITWC51RRa0VrwUXKprsoCGqVIpWd2R-5T2AMAEVbfkjgGXTDBYkC_bk4vh38kczehHLOaVxmYN_dT63o-nwgyuwK5DOxZhKCz2fWHcH0w-DB_ITWf6hB9fdUl-f__2a7Mtd08_fm6-7korOBtLY6GTrbDQCEdFRw2t2sa16FoJUlXMCAqGWSsbx9FaBl1VC1lZrBCbtq75knw67z3G8HfCNOqDT_MnZsAwJa0kZBc4Ze-TnDdcMZjJhzdJKhXNbM2qjMozamNIKWKnj9EfTDxpCnqOQ-_1JQ49x6GB5VJ5cPV6Y2oP6P6PXfzPwOMZwOzes8eok_U4WHQ-ZsO1C_69Gy-UY56-</recordid><startdate>20100701</startdate><enddate>20100701</enddate><creator>Bertazzo, Sergio</creator><creator>Zambuzzi, Willian F.</creator><creator>Campos, Daniela D.P.</creator><creator>Ogeda, Thais L.</creator><creator>Ferreira, Carmen V.</creator><creator>Bertran, Celso A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>7QO</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100701</creationdate><title>Hydroxyapatite surface solubility and effect on cell adhesion</title><author>Bertazzo, Sergio ; Zambuzzi, Willian F. ; Campos, Daniela D.P. ; Ogeda, Thais L. ; Ferreira, Carmen V. ; Bertran, Celso A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-ac0f6b4c094d14f1a15b9dbedb606752a410a2cc69d3ecc20f58465ce5ee9b883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>3T3 Cells</topic><topic>Algorithms</topic><topic>Animals</topic><topic>Aqueous solutions</topic><topic>Bioactivity</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Biological</topic><topic>Biomedical materials</topic><topic>Bones</topic><topic>Calcium phosphate</topic><topic>Cell adhesion</topic><topic>Cell Adhesion - drug effects</topic><topic>Durapatite - chemistry</topic><topic>Durapatite - pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydroxyapatite</topic><topic>Kinetics</topic><topic>Mice</topic><topic>Models, Biological</topic><topic>Models, Chemical</topic><topic>Solubility</topic><topic>Solubility equilibrium</topic><topic>Surface chemistry</topic><topic>Surface modification</topic><topic>Surface Properties</topic><topic>Surgical implants</topic><topic>Transformations</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bertazzo, Sergio</creatorcontrib><creatorcontrib>Zambuzzi, Willian F.</creatorcontrib><creatorcontrib>Campos, Daniela D.P.</creatorcontrib><creatorcontrib>Ogeda, Thais L.</creatorcontrib><creatorcontrib>Ferreira, Carmen V.</creatorcontrib><creatorcontrib>Bertran, Celso A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bertazzo, Sergio</au><au>Zambuzzi, Willian F.</au><au>Campos, Daniela D.P.</au><au>Ogeda, Thais L.</au><au>Ferreira, Carmen V.</au><au>Bertran, Celso A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydroxyapatite surface solubility and effect on cell adhesion</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2010-07-01</date><risdate>2010</risdate><volume>78</volume><issue>2</issue><spage>177</spage><epage>184</epage><pages>177-184</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>In living organisms the biological hydroxyapatite is in constant contact with body fluids, such as blood serum and saliva. Thus, dissolution, solubility and precipitation take place as part of the interaction of this material with biological fluids in tissues. In this work we have obtained the solubility constant for the system formed from aqueous solutions in equilibrium with hydroxyapatite and thus indirectly obtained the composition of the modified hydroxyapatite surface. In order to check the effects of this equilibrium and of the modification that the surface of hydroxyapatite suffers in aqueous solutions, we cultured pre-osteoblasts onto hydroxyapatite discs before and after equilibrium. The results revealed key steps of the mechanism for the bioactivity of hydroxyapatite, which are the solubilization of hydroxyapatite and the equilibrium that is formed on the surface. These processes modify the hydroxyapatite surface, whose composition is changed to a new calcium phosphate compound with the chemical formula of CaHPO
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| subjects | 3T3 Cells Algorithms Animals Aqueous solutions Bioactivity Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Biological Biomedical materials Bones Calcium phosphate Cell adhesion Cell Adhesion - drug effects Durapatite - chemistry Durapatite - pharmacology Hydrogen-Ion Concentration Hydroxyapatite Kinetics Mice Models, Biological Models, Chemical Solubility Solubility equilibrium Surface chemistry Surface modification Surface Properties Surgical implants Transformations |
| title | Hydroxyapatite surface solubility and effect on cell adhesion |
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