Synergism in Replication and Translation of Messenger RNA in a Cell-Free System

Combination of the Qβ replicase reaction with the Escherichia coli cell-free translation system markedly enhances replication of a recombinant RQ-DHFR RNA consisting of the dihydrofolate reductase (DHFR) mRNA sequence inserted into RQ135-1RNA, an efficient naturally occurring Qβ replicase template....

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1993-10, Vol.90 (20), p.9325-9329
Hauptverfasser:	Morozov, Igor Y., Ugarov, Victor I., Chetverin, Alexander B., Spirin, Alexander S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriophages Base Sequence Biochemistry Cell-Free System DNA, Recombinant Electrophoresis Escherichia coli Gels Hydrogen Bonding In Vitro Techniques Messenger RNA Molecular Sequence Data Nucleic Acid Conformation Nucleotides phage Q beta Plasmids Product labeling Protein Biosynthesis Proteins Q beta Replicase - metabolism Ribonucleic acid Ribosomes RNA RNA, Messenger - metabolism RNA, Viral - genetics Tetrahydrofolate Dehydrogenase - genetics Viruses
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!