Choroidal Neovascular Membranes Express Toll-Like Receptor 3
Background: Recent evidence has suggested a role for toll-like receptor 3 (TLR3) in experimental models of age-related macular degeneration (AMD). To date, however, few data exist about TLR3 in human AMD. The purpose of this study was to investigate the expression of TLR3 in human choroidal neovascu...
Gespeichert in:
| Veröffentlicht in: | Ophthalmic research 2010-01, Vol.44 (4), p.237-241 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 241 |
|---|---|
| container_issue | 4 |
| container_start_page | 237 |
| container_title | Ophthalmic research |
| container_volume | 44 |
| creator | Maloney, Shawn C. Antecka, Emilia Orellana, Maria E. Fernandes, Bruno F. Odashiro, Alexandre N. Eghtedari, Masoomeh Burnier, Jr, Miguel N. |
| description | Background: Recent evidence has suggested a role for toll-like receptor 3 (TLR3) in experimental models of age-related macular degeneration (AMD). To date, however, few data exist about TLR3 in human AMD. The purpose of this study was to investigate the expression of TLR3 in human choroidal neovascular (CNV) membranes. Methods: Immunostaining for TLR3 was performed on sections of CNV membranes from 8 AMD patients and eyes from 4 donors without CNV. Results: All CNV membranes expressed TLR3 in retinal pigment epithelial (RPE) cells. One was classified as having strong intensity, 5 as having moderate intensity and 2 as having weak intensity. All cases had ≧30% of the RPE cells staining for TLR3, ranging from 30 to 90%. No expression of TLR3 was observed in vascular endothelial cells or fibroblasts in any CNV membrane. In the donor eyes, the RPE cells near the ora serrata stained stronger than those at the posterior pole, where no staining was observed in 3 out of 4 cases. Conclusion: TLR3 was found in all CNV membranes and was expressed exclusively in RPE cells. The observed difference in RPE staining for TLR3 in donor eyes and CNV membranes suggests a possible role for this receptor in human neovascular AMD. |
| doi_str_mv | 10.1159/000313989 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_760152452</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2180697971</sourcerecordid><originalsourceid>FETCH-LOGICAL-c332t-61f3d9a77c30f19f109b7a23e7244e0c9291034959d2a40eb66a7f7e64f1746f3</originalsourceid><addsrcrecordid>eNpd0E1Lw0AQBuBFFFurB-8iwYt4iM5-ZNcFL1LqB1QFqeewSWY1beLG3Ub037vS2oOngeGZYeYl5JDCOaWZvgAATrm-1FtkSAXjKbBMb5NhbEPKlVADshfCHCBiDbtkwEBqLZkakqvxm_OurkyTPKL7NKHsG-OTB2wLb94xJJOvzmMIycw1TTqtF5g8Y4nd0vmE75Mda5qAB-s6Ii83k9n4Lp0-3d6Pr6dpyTlbppJaXmmjVMnBUm0p6EIZxlExIRBKzTQFLnSmK2YEYCGlUVahFJYqIS0fkdPV3s67jx7DMm_rUGLTxAtdH3Il42dMZCzKk39y7nr_Ho-LKAqgjEd0tkKldyF4tHnn69b475xC_htovgk02uP1wr5osdrIvwQjOFqBhfGv6DdgPf8DNFd2HA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>762450123</pqid></control><display><type>article</type><title>Choroidal Neovascular Membranes Express Toll-Like Receptor 3</title><source>MEDLINE</source><source>Karger Journals</source><source>Alma/SFX Local Collection</source><creator>Maloney, Shawn C. ; Antecka, Emilia ; Orellana, Maria E. ; Fernandes, Bruno F. ; Odashiro, Alexandre N. ; Eghtedari, Masoomeh ; Burnier, Jr, Miguel N.</creator><creatorcontrib>Maloney, Shawn C. ; Antecka, Emilia ; Orellana, Maria E. ; Fernandes, Bruno F. ; Odashiro, Alexandre N. ; Eghtedari, Masoomeh ; Burnier, Jr, Miguel N.</creatorcontrib><description>Background: Recent evidence has suggested a role for toll-like receptor 3 (TLR3) in experimental models of age-related macular degeneration (AMD). To date, however, few data exist about TLR3 in human AMD. The purpose of this study was to investigate the expression of TLR3 in human choroidal neovascular (CNV) membranes. Methods: Immunostaining for TLR3 was performed on sections of CNV membranes from 8 AMD patients and eyes from 4 donors without CNV. Results: All CNV membranes expressed TLR3 in retinal pigment epithelial (RPE) cells. One was classified as having strong intensity, 5 as having moderate intensity and 2 as having weak intensity. All cases had ≧30% of the RPE cells staining for TLR3, ranging from 30 to 90%. No expression of TLR3 was observed in vascular endothelial cells or fibroblasts in any CNV membrane. In the donor eyes, the RPE cells near the ora serrata stained stronger than those at the posterior pole, where no staining was observed in 3 out of 4 cases. Conclusion: TLR3 was found in all CNV membranes and was expressed exclusively in RPE cells. The observed difference in RPE staining for TLR3 in donor eyes and CNV membranes suggests a possible role for this receptor in human neovascular AMD.</description><identifier>ISSN: 0030-3747</identifier><identifier>EISSN: 1423-0259</identifier><identifier>DOI: 10.1159/000313989</identifier><identifier>PMID: 20699627</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Aged ; Aged, 80 and over ; Choroidal Neovascularization - metabolism ; Female ; Humans ; Immunoenzyme Techniques ; Macular Degeneration - metabolism ; Male ; Middle Aged ; Original Paper ; Retinal Pigment Epithelium - metabolism ; Tissue Donors ; Toll-Like Receptor 3 - metabolism</subject><ispartof>Ophthalmic research, 2010-01, Vol.44 (4), p.237-241</ispartof><rights>2010 S. Karger AG, Basel</rights><rights>Copyright © 2010 S. Karger AG, Basel.</rights><rights>Copyright (c) 2010 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-61f3d9a77c30f19f109b7a23e7244e0c9291034959d2a40eb66a7f7e64f1746f3</citedby><cites>FETCH-LOGICAL-c332t-61f3d9a77c30f19f109b7a23e7244e0c9291034959d2a40eb66a7f7e64f1746f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2422,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20699627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maloney, Shawn C.</creatorcontrib><creatorcontrib>Antecka, Emilia</creatorcontrib><creatorcontrib>Orellana, Maria E.</creatorcontrib><creatorcontrib>Fernandes, Bruno F.</creatorcontrib><creatorcontrib>Odashiro, Alexandre N.</creatorcontrib><creatorcontrib>Eghtedari, Masoomeh</creatorcontrib><creatorcontrib>Burnier, Jr, Miguel N.</creatorcontrib><title>Choroidal Neovascular Membranes Express Toll-Like Receptor 3</title><title>Ophthalmic research</title><addtitle>Ophthalmic Res</addtitle><description>Background: Recent evidence has suggested a role for toll-like receptor 3 (TLR3) in experimental models of age-related macular degeneration (AMD). To date, however, few data exist about TLR3 in human AMD. The purpose of this study was to investigate the expression of TLR3 in human choroidal neovascular (CNV) membranes. Methods: Immunostaining for TLR3 was performed on sections of CNV membranes from 8 AMD patients and eyes from 4 donors without CNV. Results: All CNV membranes expressed TLR3 in retinal pigment epithelial (RPE) cells. One was classified as having strong intensity, 5 as having moderate intensity and 2 as having weak intensity. All cases had ≧30% of the RPE cells staining for TLR3, ranging from 30 to 90%. No expression of TLR3 was observed in vascular endothelial cells or fibroblasts in any CNV membrane. In the donor eyes, the RPE cells near the ora serrata stained stronger than those at the posterior pole, where no staining was observed in 3 out of 4 cases. Conclusion: TLR3 was found in all CNV membranes and was expressed exclusively in RPE cells. The observed difference in RPE staining for TLR3 in donor eyes and CNV membranes suggests a possible role for this receptor in human neovascular AMD.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Choroidal Neovascularization - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Macular Degeneration - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Original Paper</subject><subject>Retinal Pigment Epithelium - metabolism</subject><subject>Tissue Donors</subject><subject>Toll-Like Receptor 3 - metabolism</subject><issn>0030-3747</issn><issn>1423-0259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpd0E1Lw0AQBuBFFFurB-8iwYt4iM5-ZNcFL1LqB1QFqeewSWY1beLG3Ub037vS2oOngeGZYeYl5JDCOaWZvgAATrm-1FtkSAXjKbBMb5NhbEPKlVADshfCHCBiDbtkwEBqLZkakqvxm_OurkyTPKL7NKHsG-OTB2wLb94xJJOvzmMIycw1TTqtF5g8Y4nd0vmE75Mda5qAB-s6Ii83k9n4Lp0-3d6Pr6dpyTlbppJaXmmjVMnBUm0p6EIZxlExIRBKzTQFLnSmK2YEYCGlUVahFJYqIS0fkdPV3s67jx7DMm_rUGLTxAtdH3Il42dMZCzKk39y7nr_Ho-LKAqgjEd0tkKldyF4tHnn69b475xC_htovgk02uP1wr5osdrIvwQjOFqBhfGv6DdgPf8DNFd2HA</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Maloney, Shawn C.</creator><creator>Antecka, Emilia</creator><creator>Orellana, Maria E.</creator><creator>Fernandes, Bruno F.</creator><creator>Odashiro, Alexandre N.</creator><creator>Eghtedari, Masoomeh</creator><creator>Burnier, Jr, Miguel N.</creator><general>S. Karger AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20100101</creationdate><title>Choroidal Neovascular Membranes Express Toll-Like Receptor 3</title><author>Maloney, Shawn C. ; Antecka, Emilia ; Orellana, Maria E. ; Fernandes, Bruno F. ; Odashiro, Alexandre N. ; Eghtedari, Masoomeh ; Burnier, Jr, Miguel N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-61f3d9a77c30f19f109b7a23e7244e0c9291034959d2a40eb66a7f7e64f1746f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Choroidal Neovascularization - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Macular Degeneration - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Original Paper</topic><topic>Retinal Pigment Epithelium - metabolism</topic><topic>Tissue Donors</topic><topic>Toll-Like Receptor 3 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maloney, Shawn C.</creatorcontrib><creatorcontrib>Antecka, Emilia</creatorcontrib><creatorcontrib>Orellana, Maria E.</creatorcontrib><creatorcontrib>Fernandes, Bruno F.</creatorcontrib><creatorcontrib>Odashiro, Alexandre N.</creatorcontrib><creatorcontrib>Eghtedari, Masoomeh</creatorcontrib><creatorcontrib>Burnier, Jr, Miguel N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Ophthalmic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maloney, Shawn C.</au><au>Antecka, Emilia</au><au>Orellana, Maria E.</au><au>Fernandes, Bruno F.</au><au>Odashiro, Alexandre N.</au><au>Eghtedari, Masoomeh</au><au>Burnier, Jr, Miguel N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Choroidal Neovascular Membranes Express Toll-Like Receptor 3</atitle><jtitle>Ophthalmic research</jtitle><addtitle>Ophthalmic Res</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>44</volume><issue>4</issue><spage>237</spage><epage>241</epage><pages>237-241</pages><issn>0030-3747</issn><eissn>1423-0259</eissn><abstract>Background: Recent evidence has suggested a role for toll-like receptor 3 (TLR3) in experimental models of age-related macular degeneration (AMD). To date, however, few data exist about TLR3 in human AMD. The purpose of this study was to investigate the expression of TLR3 in human choroidal neovascular (CNV) membranes. Methods: Immunostaining for TLR3 was performed on sections of CNV membranes from 8 AMD patients and eyes from 4 donors without CNV. Results: All CNV membranes expressed TLR3 in retinal pigment epithelial (RPE) cells. One was classified as having strong intensity, 5 as having moderate intensity and 2 as having weak intensity. All cases had ≧30% of the RPE cells staining for TLR3, ranging from 30 to 90%. No expression of TLR3 was observed in vascular endothelial cells or fibroblasts in any CNV membrane. In the donor eyes, the RPE cells near the ora serrata stained stronger than those at the posterior pole, where no staining was observed in 3 out of 4 cases. Conclusion: TLR3 was found in all CNV membranes and was expressed exclusively in RPE cells. The observed difference in RPE staining for TLR3 in donor eyes and CNV membranes suggests a possible role for this receptor in human neovascular AMD.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>20699627</pmid><doi>10.1159/000313989</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0030-3747 |
| ispartof | Ophthalmic research, 2010-01, Vol.44 (4), p.237-241 |
| issn | 0030-3747 1423-0259 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_760152452 |
| source | MEDLINE; Karger Journals; Alma/SFX Local Collection |
| subjects | Aged Aged, 80 and over Choroidal Neovascularization - metabolism Female Humans Immunoenzyme Techniques Macular Degeneration - metabolism Male Middle Aged Original Paper Retinal Pigment Epithelium - metabolism Tissue Donors Toll-Like Receptor 3 - metabolism |
| title | Choroidal Neovascular Membranes Express Toll-Like Receptor 3 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T19%3A37%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Choroidal%20Neovascular%20Membranes%20Express%20Toll-Like%20Receptor%203&rft.jtitle=Ophthalmic%20research&rft.au=Maloney,%20Shawn%20C.&rft.date=2010-01-01&rft.volume=44&rft.issue=4&rft.spage=237&rft.epage=241&rft.pages=237-241&rft.issn=0030-3747&rft.eissn=1423-0259&rft_id=info:doi/10.1159/000313989&rft_dat=%3Cproquest_cross%3E2180697971%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=762450123&rft_id=info:pmid/20699627&rfr_iscdi=true |