Radiation-Induced Apoptosis Measured in TK6 Human B Lymphoblast Cells Using the Comet Assay
The comet assay, a sensitive method of measuring DNA strand breaks in individuals cells, is also capable of identifying apoptotic cells which contain highly fragmented DNA. This method requires embedding cells in agarose, lysing cells to remove proteins, and providing a brief exposure to an electric...
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| Veröffentlicht in: | Radiation research 1993-10, Vol.136 (1), p.130-136 |
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| creator | Olive, Peggy L. Frazer, Garnet Banáth, Judit P. |
| description | The comet assay, a sensitive method of measuring DNA strand breaks in individuals cells, is also capable of identifying apoptotic cells which contain highly fragmented DNA. This method requires embedding cells in agarose, lysing cells to remove proteins, and providing a brief exposure to an electric field to allow broken pieces of DNA to migrate. TK6 human B lymphoblast cells undergo fragmentation which is dependent on both time after irradiation and radiation dose. While some TK6 cells undergo apoptosis within 2 h after irradiation, the fragmentation rate increases approximately 10 h after exposure to radiation doses of 2.5 to 15 Gy. Results confirm that apoptosis is a very rapid event since few cells with intermediate amounts of DNA damage were detected. The comet assay detected apoptotic TK6 cells much earlier than a flow cytometry method. |
| doi_str_mv | 10.2307/3578650 |
| format | Article |
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This method requires embedding cells in agarose, lysing cells to remove proteins, and providing a brief exposure to an electric field to allow broken pieces of DNA to migrate. TK6 human B lymphoblast cells undergo fragmentation which is dependent on both time after irradiation and radiation dose. While some TK6 cells undergo apoptosis within 2 h after irradiation, the fragmentation rate increases approximately 10 h after exposure to radiation doses of 2.5 to 15 Gy. Results confirm that apoptosis is a very rapid event since few cells with intermediate amounts of DNA damage were detected. The comet assay detected apoptotic TK6 cells much earlier than a flow cytometry method.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3578650</identifier><identifier>PMID: 8210329</identifier><identifier>CODEN: RAREAE</identifier><language>eng</language><publisher>Oak Brook, Il: Academic Press, Inc</publisher><subject>Apoptosis ; Apoptosis - radiation effects ; B-Lymphocytes - radiation effects ; Biological and medical sciences ; Biological effects of radiation ; Cell cycle ; Cell Cycle - radiation effects ; Cell lines ; Cell nucleus ; Cells ; Cells, Cultured ; CHO cells ; Comets ; DNA ; DNA Damage ; Flow Cytometry ; Fundamental and applied biological sciences. 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This method requires embedding cells in agarose, lysing cells to remove proteins, and providing a brief exposure to an electric field to allow broken pieces of DNA to migrate. TK6 human B lymphoblast cells undergo fragmentation which is dependent on both time after irradiation and radiation dose. While some TK6 cells undergo apoptosis within 2 h after irradiation, the fragmentation rate increases approximately 10 h after exposure to radiation doses of 2.5 to 15 Gy. Results confirm that apoptosis is a very rapid event since few cells with intermediate amounts of DNA damage were detected. The comet assay detected apoptotic TK6 cells much earlier than a flow cytometry method.</description><subject>Apoptosis</subject><subject>Apoptosis - radiation effects</subject><subject>B-Lymphocytes - radiation effects</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Cell cycle</subject><subject>Cell Cycle - radiation effects</subject><subject>Cell lines</subject><subject>Cell nucleus</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>CHO cells</subject><subject>Comets</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Ionizing radiations</subject><subject>Irradiation</subject><subject>Rapid Communication</subject><subject>Tissues, organs and organisms biophysics</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE9Pg0AQxTdGU2s1fgKTPRg9obO7LLDHStQaa0xMe_JAlmWxNMAiA4d-ezEl9eRp_rxf3kweIZcM7riA8F7IMAokHJEpUyLypA_-MZkCCOGFMgpPyRniFoaZBWpCJhFnILiaks8PnRW6K1ztvdRZb2xG541rOocF0jersW-HVVHT1WtAF32la_pAl7uq2bi01NjR2JYl0jUW9RftNpbGrrIdnSPq3Tk5yXWJ9mKsM7J-elzFC2_5_vwSz5ee8QE6L1OpAJPnQodcqwgkGxompQjBBgxsqFnGhWXMZopzI63wc2XSTAXKB2ZzMSM3e9-mdd-9xS6pCjTDX7q2rsckDID5gfQH8HYPmtYhtjZPmraodLtLGCS_MSZjjAN5NVr2aWWzAzfmNujXo67R6DJvdW0KPGAi4iIA_odtsXPtv9d-AHp7gqY</recordid><startdate>19931001</startdate><enddate>19931001</enddate><creator>Olive, Peggy L.</creator><creator>Frazer, Garnet</creator><creator>Banáth, Judit P.</creator><general>Academic Press, Inc</general><general>Radiation Research Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931001</creationdate><title>Radiation-Induced Apoptosis Measured in TK6 Human B Lymphoblast Cells Using the Comet Assay</title><author>Olive, Peggy L. ; Frazer, Garnet ; Banáth, Judit P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-d9b30cff3a72a98051a72155370e610e7a1d23e11ed922c5e34f9cbd969401ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Apoptosis</topic><topic>Apoptosis - radiation effects</topic><topic>B-Lymphocytes - radiation effects</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Cell cycle</topic><topic>Cell Cycle - radiation effects</topic><topic>Cell lines</topic><topic>Cell nucleus</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>CHO cells</topic><topic>Comets</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Ionizing radiations</topic><topic>Irradiation</topic><topic>Rapid Communication</topic><topic>Tissues, organs and organisms biophysics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olive, Peggy L.</creatorcontrib><creatorcontrib>Frazer, Garnet</creatorcontrib><creatorcontrib>Banáth, Judit P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olive, Peggy L.</au><au>Frazer, Garnet</au><au>Banáth, Judit P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radiation-Induced Apoptosis Measured in TK6 Human B Lymphoblast Cells Using the Comet Assay</atitle><jtitle>Radiation research</jtitle><addtitle>Radiat Res</addtitle><date>1993-10-01</date><risdate>1993</risdate><volume>136</volume><issue>1</issue><spage>130</spage><epage>136</epage><pages>130-136</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><coden>RAREAE</coden><abstract>The comet assay, a sensitive method of measuring DNA strand breaks in individuals cells, is also capable of identifying apoptotic cells which contain highly fragmented DNA. This method requires embedding cells in agarose, lysing cells to remove proteins, and providing a brief exposure to an electric field to allow broken pieces of DNA to migrate. TK6 human B lymphoblast cells undergo fragmentation which is dependent on both time after irradiation and radiation dose. While some TK6 cells undergo apoptosis within 2 h after irradiation, the fragmentation rate increases approximately 10 h after exposure to radiation doses of 2.5 to 15 Gy. Results confirm that apoptosis is a very rapid event since few cells with intermediate amounts of DNA damage were detected. The comet assay detected apoptotic TK6 cells much earlier than a flow cytometry method.</abstract><cop>Oak Brook, Il</cop><pub>Academic Press, Inc</pub><pmid>8210329</pmid><doi>10.2307/3578650</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Radiation research, 1993-10, Vol.136 (1), p.130-136 |
| issn | 0033-7587 1938-5404 |
| language | eng |
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| source | MEDLINE; Jstor Complete Legacy |
| subjects | Apoptosis Apoptosis - radiation effects B-Lymphocytes - radiation effects Biological and medical sciences Biological effects of radiation Cell cycle Cell Cycle - radiation effects Cell lines Cell nucleus Cells Cells, Cultured CHO cells Comets DNA DNA Damage Flow Cytometry Fundamental and applied biological sciences. Psychology Humans Ionizing radiations Irradiation Rapid Communication Tissues, organs and organisms biophysics |
| title | Radiation-Induced Apoptosis Measured in TK6 Human B Lymphoblast Cells Using the Comet Assay |
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