Interaction of Mouse Mammary Epithelial Cells with Collagen Substrata: Regulation of Casein Gene Expression and Secretion
Mouse mammary epithelial cells (MMEC) secrete certain milk proteins only when cultured on floating collagen gels. We demonstrate here that modulation of milk proteins by substrata is manifested at several regulatory levels; (i) Cells cultured on floating collagen gels have 3- to 10-fold more casein...
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| Veröffentlicht in: | Proc. Natl. Acad. Sci. U.S.A.; (United States) 1985-03, Vol.82 (5), p.1419-1423 |
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| container_title | Proc. Natl. Acad. Sci. U.S.A.; (United States) |
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| creator | Eva Yue-Hwa P. Lee Lee, Wen-Hwa Kaetzei, Charlotte S. Parry, Gordon Bissell, Mina J. |
| description | Mouse mammary epithelial cells (MMEC) secrete certain milk proteins only when cultured on floating collagen gels. We demonstrate here that modulation of milk proteins by substrata is manifested at several regulatory levels; (i) Cells cultured on floating collagen gels have 3- to 10-fold more casein mRNA than cells cultured on plastic or attached collagen gels. (ii) Cells on the latter two ``flat'' substrata, nevertheless, synthesize a significant amount of caseins, indicating that the remaining mRNA is functional. (iii) Cells on all substrata are inducible for casein mRNA and casein proteins by prolactin, but the extent of induction is greater on collagen than that on plastic--i.e., the substratum confers an altered degree of inducibility. (iv) Cells on all substrata synthesize casein proteins at rates proportional to the amount of casein mRNA, but the newly synthesized caseins in cells on plastic are degraded intracellularly, whereas those synthesized by cells on floating gels are secreted into the medium. (v) Cells on all substrata examined lose virtually all mRNA for whey acidic protein despite the fact that this mRNA is abundant in the mammary gland itself; we conclude that additional, as-yet-unknown, factors are necessary for synthesis and secretion of whey acidic protein in culture. |
| doi_str_mv | 10.1073/pnas.82.5.1419 |
| format | Article |
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Lee ; Lee, Wen-Hwa ; Kaetzei, Charlotte S. ; Parry, Gordon ; Bissell, Mina J.</creator><creatorcontrib>Eva Yue-Hwa P. Lee ; Lee, Wen-Hwa ; Kaetzei, Charlotte S. ; Parry, Gordon ; Bissell, Mina J. ; Univ. of California, Berkeley</creatorcontrib><description>Mouse mammary epithelial cells (MMEC) secrete certain milk proteins only when cultured on floating collagen gels. We demonstrate here that modulation of milk proteins by substrata is manifested at several regulatory levels; (i) Cells cultured on floating collagen gels have 3- to 10-fold more casein mRNA than cells cultured on plastic or attached collagen gels. (ii) Cells on the latter two ``flat'' substrata, nevertheless, synthesize a significant amount of caseins, indicating that the remaining mRNA is functional. (iii) Cells on all substrata are inducible for casein mRNA and casein proteins by prolactin, but the extent of induction is greater on collagen than that on plastic--i.e., the substratum confers an altered degree of inducibility. (iv) Cells on all substrata synthesize casein proteins at rates proportional to the amount of casein mRNA, but the newly synthesized caseins in cells on plastic are degraded intracellularly, whereas those synthesized by cells on floating gels are secreted into the medium. (v) Cells on all substrata examined lose virtually all mRNA for whey acidic protein despite the fact that this mRNA is abundant in the mammary gland itself; we conclude that additional, as-yet-unknown, factors are necessary for synthesis and secretion of whey acidic protein in culture.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.5.1419</identifier><identifier>PMID: 3856271</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>550200 - Biochemistry ; Animal cells ; ANIMAL TISSUES ; ANIMALS ; BASIC BIOLOGICAL SCIENCES ; BIOCHEMICAL REACTION KINETICS ; BIOCHEMISTRY ; Biological and medical sciences ; BIOSYNTHESIS ; BODY ; CASEIN ; Caseins - biosynthesis ; Caseins - genetics ; Caseins - metabolism ; Cell culture techniques ; CELL CULTURES ; Cell cultures. Hybridization. Fusion ; Cells ; Cells, Cultured ; CHEMISTRY ; COLLAGEN ; Collagen - physiology ; Collagens ; CULTURE MEDIA ; Cultured cells ; Epithelial cells ; EPITHELIUM ; Epithelium - physiology ; Extracellular Matrix - physiology ; Fundamental and applied biological sciences. Psychology ; Gels ; Gene Expression Regulation ; GENES ; GLANDS ; GONADOTROPINS ; HORMONES ; INDUSTRIAL WASTES ; KINETICS ; LTH ; MAMMALS ; MAMMARY GLANDS ; Mammary Glands, Animal - physiology ; MATERIALS ; MESSENGER-RNA ; MICE ; Milk protein ; Molecular and cellular biology ; NUCLEIC ACIDS ; ORGANIC COMPOUNDS ; ORGANIC PHOSPHORUS COMPOUNDS ; ORGANS ; PEPTIDE HORMONES ; PETROCHEMICALS ; PETROLEUM PRODUCTS ; PITUITARY HORMONES ; PLASTICS ; Prolactin - pharmacology ; PROTEINS ; REACTION KINETICS ; RNA ; RNA, Messenger - genetics ; RODENTS ; SCLEROPROTEINS ; Secretion ; SUBSTRATES ; SYNTHESIS ; SYNTHETIC MATERIALS ; TISSUES ; Transferrins ; VERTEBRATES ; WASTES ; WHEY</subject><ispartof>Proc. Natl. Acad. Sci. U.S.A.; (United States), 1985-03, Vol.82 (5), p.1419-1423</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c543t-67309c644b65e28b24850d456eb05dbfbe871d71e7031850f4a7b8040a3e20743</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/5.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25421$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25421$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9080571$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3856271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5836891$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Eva Yue-Hwa P. Lee</creatorcontrib><creatorcontrib>Lee, Wen-Hwa</creatorcontrib><creatorcontrib>Kaetzei, Charlotte S.</creatorcontrib><creatorcontrib>Parry, Gordon</creatorcontrib><creatorcontrib>Bissell, Mina J.</creatorcontrib><creatorcontrib>Univ. of California, Berkeley</creatorcontrib><title>Interaction of Mouse Mammary Epithelial Cells with Collagen Substrata: Regulation of Casein Gene Expression and Secretion</title><title>Proc. Natl. Acad. Sci. U.S.A.; (United States)</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Mouse mammary epithelial cells (MMEC) secrete certain milk proteins only when cultured on floating collagen gels. We demonstrate here that modulation of milk proteins by substrata is manifested at several regulatory levels; (i) Cells cultured on floating collagen gels have 3- to 10-fold more casein mRNA than cells cultured on plastic or attached collagen gels. (ii) Cells on the latter two ``flat'' substrata, nevertheless, synthesize a significant amount of caseins, indicating that the remaining mRNA is functional. (iii) Cells on all substrata are inducible for casein mRNA and casein proteins by prolactin, but the extent of induction is greater on collagen than that on plastic--i.e., the substratum confers an altered degree of inducibility. (iv) Cells on all substrata synthesize casein proteins at rates proportional to the amount of casein mRNA, but the newly synthesized caseins in cells on plastic are degraded intracellularly, whereas those synthesized by cells on floating gels are secreted into the medium. (v) Cells on all substrata examined lose virtually all mRNA for whey acidic protein despite the fact that this mRNA is abundant in the mammary gland itself; we conclude that additional, as-yet-unknown, factors are necessary for synthesis and secretion of whey acidic protein in culture.</description><subject>550200 - Biochemistry</subject><subject>Animal cells</subject><subject>ANIMAL TISSUES</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOCHEMICAL REACTION KINETICS</subject><subject>BIOCHEMISTRY</subject><subject>Biological and medical sciences</subject><subject>BIOSYNTHESIS</subject><subject>BODY</subject><subject>CASEIN</subject><subject>Caseins - biosynthesis</subject><subject>Caseins - genetics</subject><subject>Caseins - metabolism</subject><subject>Cell culture techniques</subject><subject>CELL CULTURES</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>CHEMISTRY</subject><subject>COLLAGEN</subject><subject>Collagen - physiology</subject><subject>Collagens</subject><subject>CULTURE MEDIA</subject><subject>Cultured cells</subject><subject>Epithelial cells</subject><subject>EPITHELIUM</subject><subject>Epithelium - physiology</subject><subject>Extracellular Matrix - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Gene Expression Regulation</subject><subject>GENES</subject><subject>GLANDS</subject><subject>GONADOTROPINS</subject><subject>HORMONES</subject><subject>INDUSTRIAL WASTES</subject><subject>KINETICS</subject><subject>LTH</subject><subject>MAMMALS</subject><subject>MAMMARY GLANDS</subject><subject>Mammary Glands, Animal - physiology</subject><subject>MATERIALS</subject><subject>MESSENGER-RNA</subject><subject>MICE</subject><subject>Milk protein</subject><subject>Molecular and cellular biology</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC PHOSPHORUS COMPOUNDS</subject><subject>ORGANS</subject><subject>PEPTIDE HORMONES</subject><subject>PETROCHEMICALS</subject><subject>PETROLEUM PRODUCTS</subject><subject>PITUITARY HORMONES</subject><subject>PLASTICS</subject><subject>Prolactin - pharmacology</subject><subject>PROTEINS</subject><subject>REACTION KINETICS</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RODENTS</subject><subject>SCLEROPROTEINS</subject><subject>Secretion</subject><subject>SUBSTRATES</subject><subject>SYNTHESIS</subject><subject>SYNTHETIC MATERIALS</subject><subject>TISSUES</subject><subject>Transferrins</subject><subject>VERTEBRATES</subject><subject>WASTES</subject><subject>WHEY</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LEzEYxgdR1rp69SAIQcRbaz4nGcGDDHVd2EVw9Rwy6TttljSpk4zu_vdmaLdUEDyF8Hue9-upqpcELwiW7P0umLRQdCEWhJPmUTUjuCHzmjf4cTXDmMq54pQ_rZ6ldIsxboTCZ9UZU6Kmksyq-8uQYTA2uxhQ7NF1HBOga7PdmuEeLXcub8A741EL3if0u_xRG703awjoZuxSHkw2H9A3WI_ePFRpTQIX0AUEQMu73QApTcSEFboBO8Cke1496Y1P8OLwnlc_Pi-_t1_mV18vLttPV3MrOMvzWjLc2JrzrhZAVUe5EnjFRQ0dFquu70BJspIEJGakoJ4b2SnMsWFAseTsvPq4r7sbuy2sLIQyste7wU0r6mic_psEt9Hr-EuzRlLJiv_N3h9TdjpZl8FubAwBbNZCsVo1pIjeHZoM8ecIKeutS7aczAQoF9WyxoTiRv1XWEJUqhZTxcVeaIeY0gD9cWKC9ZS8npLXimoxmZpieH2651F-iLrwtwdukjW-H0ywLh1lDVZYyNNNpvIP9NhG96P3Ge7ySb9_Cgt_tee3KcfhKKCCU8L-ABd71_U</recordid><startdate>19850301</startdate><enddate>19850301</enddate><creator>Eva Yue-Hwa P. Lee</creator><creator>Lee, Wen-Hwa</creator><creator>Kaetzei, Charlotte S.</creator><creator>Parry, Gordon</creator><creator>Bissell, Mina J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19850301</creationdate><title>Interaction of Mouse Mammary Epithelial Cells with Collagen Substrata: Regulation of Casein Gene Expression and Secretion</title><author>Eva Yue-Hwa P. Lee ; Lee, Wen-Hwa ; Kaetzei, Charlotte S. ; Parry, Gordon ; Bissell, Mina J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c543t-67309c644b65e28b24850d456eb05dbfbe871d71e7031850f4a7b8040a3e20743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>550200 - Biochemistry</topic><topic>Animal cells</topic><topic>ANIMAL TISSUES</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOCHEMICAL REACTION KINETICS</topic><topic>BIOCHEMISTRY</topic><topic>Biological and medical sciences</topic><topic>BIOSYNTHESIS</topic><topic>BODY</topic><topic>CASEIN</topic><topic>Caseins - biosynthesis</topic><topic>Caseins - genetics</topic><topic>Caseins - metabolism</topic><topic>Cell culture techniques</topic><topic>CELL CULTURES</topic><topic>Cell cultures. Hybridization. Fusion</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>CHEMISTRY</topic><topic>COLLAGEN</topic><topic>Collagen - physiology</topic><topic>Collagens</topic><topic>CULTURE MEDIA</topic><topic>Cultured cells</topic><topic>Epithelial cells</topic><topic>EPITHELIUM</topic><topic>Epithelium - physiology</topic><topic>Extracellular Matrix - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Gene Expression Regulation</topic><topic>GENES</topic><topic>GLANDS</topic><topic>GONADOTROPINS</topic><topic>HORMONES</topic><topic>INDUSTRIAL WASTES</topic><topic>KINETICS</topic><topic>LTH</topic><topic>MAMMALS</topic><topic>MAMMARY GLANDS</topic><topic>Mammary Glands, Animal - physiology</topic><topic>MATERIALS</topic><topic>MESSENGER-RNA</topic><topic>MICE</topic><topic>Milk protein</topic><topic>Molecular and cellular biology</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC PHOSPHORUS COMPOUNDS</topic><topic>ORGANS</topic><topic>PEPTIDE HORMONES</topic><topic>PETROCHEMICALS</topic><topic>PETROLEUM PRODUCTS</topic><topic>PITUITARY HORMONES</topic><topic>PLASTICS</topic><topic>Prolactin - pharmacology</topic><topic>PROTEINS</topic><topic>REACTION KINETICS</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RODENTS</topic><topic>SCLEROPROTEINS</topic><topic>Secretion</topic><topic>SUBSTRATES</topic><topic>SYNTHESIS</topic><topic>SYNTHETIC MATERIALS</topic><topic>TISSUES</topic><topic>Transferrins</topic><topic>VERTEBRATES</topic><topic>WASTES</topic><topic>WHEY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eva Yue-Hwa P. Lee</creatorcontrib><creatorcontrib>Lee, Wen-Hwa</creatorcontrib><creatorcontrib>Kaetzei, Charlotte S.</creatorcontrib><creatorcontrib>Parry, Gordon</creatorcontrib><creatorcontrib>Bissell, Mina J.</creatorcontrib><creatorcontrib>Univ. of California, Berkeley</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eva Yue-Hwa P. Lee</au><au>Lee, Wen-Hwa</au><au>Kaetzei, Charlotte S.</au><au>Parry, Gordon</au><au>Bissell, Mina J.</au><aucorp>Univ. of California, Berkeley</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of Mouse Mammary Epithelial Cells with Collagen Substrata: Regulation of Casein Gene Expression and Secretion</atitle><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-03-01</date><risdate>1985</risdate><volume>82</volume><issue>5</issue><spage>1419</spage><epage>1423</epage><pages>1419-1423</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Mouse mammary epithelial cells (MMEC) secrete certain milk proteins only when cultured on floating collagen gels. We demonstrate here that modulation of milk proteins by substrata is manifested at several regulatory levels; (i) Cells cultured on floating collagen gels have 3- to 10-fold more casein mRNA than cells cultured on plastic or attached collagen gels. (ii) Cells on the latter two ``flat'' substrata, nevertheless, synthesize a significant amount of caseins, indicating that the remaining mRNA is functional. (iii) Cells on all substrata are inducible for casein mRNA and casein proteins by prolactin, but the extent of induction is greater on collagen than that on plastic--i.e., the substratum confers an altered degree of inducibility. (iv) Cells on all substrata synthesize casein proteins at rates proportional to the amount of casein mRNA, but the newly synthesized caseins in cells on plastic are degraded intracellularly, whereas those synthesized by cells on floating gels are secreted into the medium. (v) Cells on all substrata examined lose virtually all mRNA for whey acidic protein despite the fact that this mRNA is abundant in the mammary gland itself; we conclude that additional, as-yet-unknown, factors are necessary for synthesis and secretion of whey acidic protein in culture.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3856271</pmid><doi>10.1073/pnas.82.5.1419</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proc. Natl. Acad. Sci. U.S.A.; (United States), 1985-03, Vol.82 (5), p.1419-1423 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76012098 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 550200 - Biochemistry Animal cells ANIMAL TISSUES ANIMALS BASIC BIOLOGICAL SCIENCES BIOCHEMICAL REACTION KINETICS BIOCHEMISTRY Biological and medical sciences BIOSYNTHESIS BODY CASEIN Caseins - biosynthesis Caseins - genetics Caseins - metabolism Cell culture techniques CELL CULTURES Cell cultures. Hybridization. Fusion Cells Cells, Cultured CHEMISTRY COLLAGEN Collagen - physiology Collagens CULTURE MEDIA Cultured cells Epithelial cells EPITHELIUM Epithelium - physiology Extracellular Matrix - physiology Fundamental and applied biological sciences. Psychology Gels Gene Expression Regulation GENES GLANDS GONADOTROPINS HORMONES INDUSTRIAL WASTES KINETICS LTH MAMMALS MAMMARY GLANDS Mammary Glands, Animal - physiology MATERIALS MESSENGER-RNA MICE Milk protein Molecular and cellular biology NUCLEIC ACIDS ORGANIC COMPOUNDS ORGANIC PHOSPHORUS COMPOUNDS ORGANS PEPTIDE HORMONES PETROCHEMICALS PETROLEUM PRODUCTS PITUITARY HORMONES PLASTICS Prolactin - pharmacology PROTEINS REACTION KINETICS RNA RNA, Messenger - genetics RODENTS SCLEROPROTEINS Secretion SUBSTRATES SYNTHESIS SYNTHETIC MATERIALS TISSUES Transferrins VERTEBRATES WASTES WHEY |
| title | Interaction of Mouse Mammary Epithelial Cells with Collagen Substrata: Regulation of Casein Gene Expression and Secretion |
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