Analysis of genetic mosaics in developing and adult Drosophila tissues
We have constructed a series of strains to facilitate the generation and analysis of clones of genetically distinct cells in developing and adult tissues of Drosophila. Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a major chromosome arm, as...
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| Veröffentlicht in: | Development (Cambridge) 1993-04, Vol.117 (4), p.1223-1237 |
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| container_title | Development (Cambridge) |
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| creator | Xu, T Rubin, G M |
| description | We have constructed a series of strains to facilitate the generation and analysis of clones of genetically distinct cells in developing and adult tissues of Drosophila. Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a major chromosome arm, as well as a gratuitous cell-autonomous marker. Novel markers that carry epitope tags and that are localized to either the cell nucleus or cell membrane have been generated. As a demonstration of how these strains can be used to study a particular gene, we have analyzed the developmental role of the Drosophila EGF receptor homolog. Moreover, we have shown that these strains can be utilized to identify new mutations in mosaic animals in an efficient and unbiased way, thereby providing an unprecedented opportunity to perform systematic genetic screens for mutations affecting many biological processes. |
| doi_str_mv | 10.1242/dev.117.4.1223 |
| format | Article |
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Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a major chromosome arm, as well as a gratuitous cell-autonomous marker. Novel markers that carry epitope tags and that are localized to either the cell nucleus or cell membrane have been generated. As a demonstration of how these strains can be used to study a particular gene, we have analyzed the developmental role of the Drosophila EGF receptor homolog. Moreover, we have shown that these strains can be utilized to identify new mutations in mosaic animals in an efficient and unbiased way, thereby providing an unprecedented opportunity to perform systematic genetic screens for mutations affecting many biological processes.</description><identifier>ISSN: 0950-1991</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.117.4.1223</identifier><identifier>PMID: 8404527</identifier><language>eng</language><publisher>Cambridge: The Company of Biologists Limited</publisher><subject>Animals ; Biological and medical sciences ; Drosophila - anatomy & histology ; Drosophila - embryology ; Drosophila - genetics ; Drosophila melanogaster ; Embryo, Nonmammalian - physiology ; ErbB Receptors - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Genetic Markers ; Genetic Testing - methods ; Immunohistochemistry ; Insecta ; Invertebrates ; Life cycle. Embryology. Development ; Male ; Mosaicism - genetics ; Mutation - genetics ; Phenotype ; Physiology. 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Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a major chromosome arm, as well as a gratuitous cell-autonomous marker. Novel markers that carry epitope tags and that are localized to either the cell nucleus or cell membrane have been generated. As a demonstration of how these strains can be used to study a particular gene, we have analyzed the developmental role of the Drosophila EGF receptor homolog. Moreover, we have shown that these strains can be utilized to identify new mutations in mosaic animals in an efficient and unbiased way, thereby providing an unprecedented opportunity to perform systematic genetic screens for mutations affecting many biological processes.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Drosophila - anatomy & histology</subject><subject>Drosophila - embryology</subject><subject>Drosophila - genetics</subject><subject>Drosophila melanogaster</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>ErbB Receptors - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Markers</subject><subject>Genetic Testing - methods</subject><subject>Immunohistochemistry</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>Life cycle. Embryology. Development</subject><subject>Male</subject><subject>Mosaicism - genetics</subject><subject>Mutation - genetics</subject><subject>Phenotype</subject><subject>Physiology. Development</subject><subject>Saccharomyces cerevisiae</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EglJY2ZA8ILYUO3HseERAAakSC8yW44_WyI1LLgXx73HVCLExnU73-L3zg9AFJTNasvLGus8ZpWLGcltWB2hCmRCFpKU8RBMia1JQKekJOgV4J4RUXIhjdNwwwupSTND8ttPxGwLg5PHSdW4IBq8T6GAAhw7neBfTJnRLrDuLtd3GAd_3CdJmFaLGQwDYOjhDR15HcOdjnaK3-cPr3VOxeHl8vrtdFIbVbChE3bbMcONbXsuKMl8Ta6WvnBeaOsOtcbySzlZeiNa3jsjSNrKR0jSNYPn6Kbre52769JH3DmodwLgYdefSFpTghHBO_wcpbyiTkmdwtgdN_hT0zqtNH9a6_1aUqJ1hlRWobFgxtTOcH1yOydt27ewvPirN86txrsHo6HvdmQC_GBNVw9juwGKPrcJy9RV6p9qQYloGGECN1v-u_QHGR5P5</recordid><startdate>19930401</startdate><enddate>19930401</enddate><creator>Xu, T</creator><creator>Rubin, G M</creator><general>The Company of Biologists Limited</general><general>Company of Biologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19930401</creationdate><title>Analysis of genetic mosaics in developing and adult Drosophila tissues</title><author>Xu, T ; Rubin, G M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-75bb4c6cfb659314f50dd9f3ef7a1ec6dce639ed3f77bfbe092d89899c8874003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Drosophila - anatomy & histology</topic><topic>Drosophila - embryology</topic><topic>Drosophila - genetics</topic><topic>Drosophila melanogaster</topic><topic>Embryo, Nonmammalian - physiology</topic><topic>ErbB Receptors - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Markers</topic><topic>Genetic Testing - methods</topic><topic>Immunohistochemistry</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>Life cycle. Embryology. Development</topic><topic>Male</topic><topic>Mosaicism - genetics</topic><topic>Mutation - genetics</topic><topic>Phenotype</topic><topic>Physiology. Development</topic><topic>Saccharomyces cerevisiae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, T</creatorcontrib><creatorcontrib>Rubin, G M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, T</au><au>Rubin, G M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of genetic mosaics in developing and adult Drosophila tissues</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>1993-04-01</date><risdate>1993</risdate><volume>117</volume><issue>4</issue><spage>1223</spage><epage>1237</epage><pages>1223-1237</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>We have constructed a series of strains to facilitate the generation and analysis of clones of genetically distinct cells in developing and adult tissues of Drosophila. Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a major chromosome arm, as well as a gratuitous cell-autonomous marker. Novel markers that carry epitope tags and that are localized to either the cell nucleus or cell membrane have been generated. As a demonstration of how these strains can be used to study a particular gene, we have analyzed the developmental role of the Drosophila EGF receptor homolog. Moreover, we have shown that these strains can be utilized to identify new mutations in mosaic animals in an efficient and unbiased way, thereby providing an unprecedented opportunity to perform systematic genetic screens for mutations affecting many biological processes.</abstract><cop>Cambridge</cop><pub>The Company of Biologists Limited</pub><pmid>8404527</pmid><doi>10.1242/dev.117.4.1223</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 1993-04, Vol.117 (4), p.1223-1237 |
| issn | 0950-1991 1477-9129 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76006610 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals Biological and medical sciences Drosophila - anatomy & histology Drosophila - embryology Drosophila - genetics Drosophila melanogaster Embryo, Nonmammalian - physiology ErbB Receptors - genetics Female Fundamental and applied biological sciences. Psychology Genetic Markers Genetic Testing - methods Immunohistochemistry Insecta Invertebrates Life cycle. Embryology. Development Male Mosaicism - genetics Mutation - genetics Phenotype Physiology. Development Saccharomyces cerevisiae |
| title | Analysis of genetic mosaics in developing and adult Drosophila tissues |
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